期刊：
Journal of Cellular Biochemistry,2019年120(12):19318-19329 ISSN：0730-2312
通讯作者：
Zhang, Chao;Zhu, Liguo
作者机构：
[Yang, Shaofeng; Zhang, Chao; Li, Zhaoyong; Nie, Ying; Luo, Zhenhua; Guo, Yantao] Hunan Univ Chinese Med, Hosp 1, Dept Spine, 95 Shaoshan Middle St, Changsha 410007, Hunan, Peoples R China.;[Li, Linghui; Zhu, Liguo] China Acad Chinese Med Sci, Wangjing Hosp, Dept Gen Orthoped, Huajiadi St, Beijing 100102, Peoples R China.
通讯机构：
[Zhang, Chao] H;[Zhu, Liguo] C;Hunan Univ Chinese Med, Hosp 1, Dept Spine, 95 Shaoshan Middle St, Changsha 410007, Hunan, Peoples R China.;China Acad Chinese Med Sci, Wangjing Hosp, Dept Gen Orthoped, Huajiadi St, Beijing 100102, Peoples R China.
关键词：
Bu-Shen-Huo-Xue-Fang;extracellular matrix;intervertebral disk degeneration;miR-23c;miR-483-3p;nucleus pulposus cell
摘要：
Intervertebral disk degeneration (IDD) has been widely considered as one of the main causes for low back pain, which can cause a severe impact to human health and huge economic burden to worldwide society. IDD pathogenesis can be affected by extensive degradation of extracellular matrix (ECM) and the hyperproliferation of nucleus pulposus (NP) cells. During the IDD process, expression of the ECM degradation enzymes matrix metalloproteinase and ADAMTS increases, whereas expression of ECM synthesis-related aggrecan and COL2A1 decreases. In addition, the Wnt signaling pathway is reportedly involved in the process of IDD. Bu-Shen-Huo-Xue-Fang (BSHXF), a Chinese traditional medicine formula that contains six Chinese traditional medicinal herbs, is widely used in the treatment of IDD. Herein, we obtained the serum containing BSHXF from BSHXF-fed rat and demonstrated that the BSHXF promoted NP cell proliferation and ECM synthesis through the Wnt signaling pathway. By using DIANA online tools and luciferase reporter gene assays, we confirmed that miR-483-3p and miR-23c regulated CTNNB1 and GSK3B, respectively, through direct targeting, thereby affecting the effect of BSHXF on NP cell proliferation and ECM synthesis through the Wnt signaling pathway. Taken together, we demonstrated the function and mechanism of BSHXF in regulating NP cell proliferation and ECM remodeling through the Wnt signaling pathway during IDD.
作者机构：
[李玲] College of Traditional Chinese Medicine, Hunan University of Chinese Medicine, Changsha, 410208, China;[卢芳国; 张波; 高强; 胡珏] Medical College, Hunan University of Chinese Medicine, Changsha, 410208, China;[蔡亮] Microbiology Lab, Hunan Provincial Center for Disease Control and Prevention, Changsha, 410000, China;[吴涛; 何谷良] Graduate School, Hunan University of Chinese Medicine, Changsha, 410208, China
作者机构：
[李玲] Hunan University of Traditional Chinese Medicine, Changsha, 410028, China;Hunan Provincial Key Laboratory for Prevention and Treatment of Ophthalmology and Otolaryngology Diseases with Chinese Medicine, Changsha, 410028, China;Hunan Provincial Engineering and Technological Research Center for Prevention and Treatment of Ophthalmology and Otolaryngology Diseases with Chinese Medicine and Protecting Visual Function, Changsha, 410028, China;[宋岚; 何迎春] Hunan University of Traditional Chinese Medicine, Changsha, 410028, China, Hunan Provincial Engineering and Technological Research Center for Prevention and Treatment of Ophthalmology and Otolaryngology Diseases with Chinese Medicine and Protecting Visual Function, Changsha, 410028, China;[周芳亮; 胡梅] Hunan University of Traditional Chinese Medicine, Changsha, 410028, China, Hunan Provincial Key Laboratory for Prevention and Treatment of Ophthalmology and Otolaryngology Diseases with Chinese Medicine, Changsha, 410028, China
通讯机构：
[He, Y.-C.] H;Hunan University of Traditional Chinese Medicine, Changsha, China
作者机构：
[Peng, Tingting; Tang, Qianli; Liao, Xianjiu] Youjiang Med Univ Nationalities, West Guangxi Key Lab Prevent & Treatment High Inc, 98 Chengxiang Rd, Baise 533000, Guangxi, Peoples R China.;[Li, Liqing; Ge, Bin; Tang, Qianli; Liao, Xianjiu] Hunan Univ Chinese Med, Sch Integrated Chinese & Western Med, Changsha, Hunan, Peoples R China.;[Pan, Jianbin] Nanjing Univ, Sch Chem & Chem Engn, State Key Lab Analyt Chem Life Sci, Nanjing, Jiangsu, Peoples R China.
通讯机构：
[Tang, Qianli] Y;Youjiang Med Univ Nationalities, West Guangxi Key Lab Prevent & Treatment High Inc, 98 Chengxiang Rd, Baise 533000, Guangxi, Peoples R China.
关键词：
carbon nitride nanosheet;catalytic hairpin assembly;intracellular signal amplification;microRNA;nanoprobe
摘要：
In this study, an ultrasensitive fluorescence turn-on assay for in situ sensing of intracellular microRNA (miRNA) was developed utilizing a carbon nitride nanosheet (CNNS) and a catalytic hairpin assembly (CHA). The CHA showed favourable signal amplification for low-level biomarkers, and CNNS was an excellent candidate as a fluorescence quencher and gene vector. Moreover, the hairpin DNA of CHA could be adsorbed onto the surface of CNNS. An enzyme-free fluorescence biosensor for ultrasensitive sensing of intracellular miRNA in cells based on CHA and CNNS was designed. When faced with target miRNA, the fluorescence was recovered due to the miRNA, which could trigger cycling of CHA circuits, leading to the production of a marked enhanced fluorescence signal. Compared with traditional methods, the proposed method is convenient, with low cytotoxicity, and high specificity and ultrasensitivity. It has promising potential for detection low-level biomarkers.
