作者： 李玲;朱梦晨;李点;杨婷婷;邱婷;...

期刊： 国际眼科杂志,2021年21(02):217-221 ISSN：1672-5123

通讯作者： Li, D.

作者机构： [李玲] College of Traditional Chinese Medicine, Hunan University of Chinese Medicine, Changsha, 410208, China;[杨婷婷; 邱婷; 王健; 朱梦晨] College of Integrated Traditional Chinese and Western Medicine, Hunan University of Chinese Medicine, Changsha, 410208, China;[李点] Health Management Section, The First Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, 410007, China

通讯机构： [Li, D.] H;Health Management Section, China

关键词： 兔泪腺上皮细胞;原代培养;干眼细胞模型;脂多糖;肿瘤坏死因子-α

摘要： 目的:通过体外分离及培养泪腺上皮细胞,建立干眼细胞模型,分析相关炎症因子,为进一步探究干眼治疗的有效药物奠定基础.方法:体外分离培养兔泪腺上皮细胞,并采用细胞增殖实验和免疫荧光实验对原代细胞活性和纯度进行鉴定.根据脂多糖(LPS)和肿瘤坏死因子-α(TNF-α)的IC50值,采用两者的0.5倍IC50刺激兔泪腺上皮细胞,构建干眼细胞模型即LPS组和TNF-α组,并通过细胞增殖实验、ELISA和流式方法对比两种构建干眼细胞模型的相关生物学特征.结果:兔泪腺上皮原代细胞培养12h后细胞基本贴壁,48h可见细胞形态舒展,呈长三角形.兔泪腺上皮原代细胞活性为92％以上,标志角化蛋白(Pan-cytoeratin)阳性率>90％,符合实验要求.LPS和TNF-α12h的IC50分别为20μg/mL、4.996ng/mL,采用LPS(10μg/mL)和TNF-α(2.5ng/mL)干预细胞12h后:两组细胞凋亡率明显高于空白对照组(P<0.01),组间比较,TNF-α 组细胞凋亡率高于LPS组(P<0.01);两组细胞上清液中IL-1β和IL-6的含量均明显高于空白对照组(P<0.01),组间比较,TNF-α组IL-1β 和IL-6的含量明显高于LPS组(P<0.01).提示TNF-α模拟干眼的炎症反应效果优于LPS.结论:本研究成功建立了一种细胞纯度较高、相对简便、快速,模型稳定的兔干眼细胞模型,为兔泪腺上皮细胞功能及干眼的基础研究提供了更稳定的体外实验模型.

语种： 中文

作者： Gao, Yan;Li, Jun-Tong;Li, Xiang;Li, Xun;Yang, Song-Wei;...

期刊： JOURNAL OF CELLULAR PHYSIOLOGY,2021年236(5):3832-3862 ISSN：0021-9541

通讯作者： Zhang, L

作者机构： [Li, Lin; Zhang, Lan; Gao, Yan; Li, Xiang; Zhang, L] Capital Med Univ, Dept Pharm,Beijing Inst Brain Disorders,Minist Ed, Natl Clin Res Ctr Geriatr Dis,Key Lab Neurodegene, Beijing Engn Res Ctr Nervous Syst Drugs,Xuanwu Ho, Beijing 100053, Peoples R China.;[Gao, Yan; Chen, Nai-Hong] Chinese Acad Med Sci & Peking Union Med Coll, State Key Lab Bioact Subst & Funct Nat Med, Inst Mat Med, Beijing, Peoples R China.;[Gao, Yan; Chen, Nai-Hong] Chinese Acad Med Sci & Peking Union Med Coll, Neurosci Ctr, Beijing, Peoples R China.;[Li, Jun-Tong] Guangzhou Univ Chinese Med, Sci & Technol Innovat Ctr, Guangzhou, Peoples R China.;[Li, Xun; Chen, Nai-Hong; Yang, Song-Wei] Hunan Univ Chinese Med, Coll Pharm, Changsha, Peoples R China.

通讯机构： [Zhang, L ] C;Capital Med Univ, Dept Pharm,Beijing Inst Brain Disorders,Minist Ed, Natl Clin Res Ctr Geriatr Dis,Key Lab Neurodegene, Beijing Engn Res Ctr Nervous Syst Drugs,Xuanwu Ho, Beijing 100053, Peoples R China.

关键词： acetaminophen;Luminex analysis;metabolomics;tetrahydroxy stilbene glycoside;UHPLC-Q-TOF/MS

摘要： Tetrahydroxy stilbene glycoside (TSG) is a main active compound in Polygonum multiflorum. Acetaminophen (APAP) is a well-known analgesic and antipyretic drug. It is considered to be safe within a therapeutic range, in case of acute intoxication hepatotoxicity occurs. This present study aims to observe TSG-provided alleviation on APAP-induced hepatoxicity in C57BL/6 mice. APAP performs extensive necrosis and dissolves nucleus suggesting liver damage from hepatic histopathology. Serum alanine aminotransferase, aspartate aminotransferase, lactate dehydrogenase, and alkaline phosphatase analysis and liver histological evaluation showed that TSG reduced the hepatotoxicity induced by a toxic dose of APAP. Moreover, TSG alone had no hepatotoxicity. TSG eliminated hepatic glutathione depletion and cysteine adducts formation. It also reduced the expression of interleukin-10 and lowered the production of reactive oxygen species in liver tissues. Luminex was used to detect cytokine production in different groups. Herein, we used an untargeted metabolomics approach by performing ultrahigh-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry on treated mice to identify metabolic disruptions under APAP and TSG. Major alterations were observed for purine metabolism, amino acid metabolism, and fatty acid metabolism. These data provide metabolic evidence and biomarkers in the liver that the ABC transporters, Glycine serine and threonine metabolism, and Choline metabolism in cancer changed the most. These targets of metabolites have the potential to improve our understanding of homeostatic. Meanwhile, these metabolites revealed that TSG can alleviate inflammation caused by APAP and promote the activity of intrinsic antioxidants. In summary, TSG can regulate lipid metabolism, promote the production of antioxidant enzymes, and decrease the inflammatory response.

语种： 英文

作者： 李玲;王莘智

期刊： 亚太传统医药,2020年16(03):140-143 ISSN：1673-2197

作者机构： 湖南中医药大学第一中医临床学院,湖南长沙410208;湖南中医药大学第一附属医院,湖南长沙410007;[王莘智] 中医药大学第一附属医院;[李玲] 湖南中医药大学

关键词： 类风湿关节炎;雷公藤片;寒热;痹证

摘要： 目的:观察雷公藤片对类风湿关节寒湿痹阻证(寒痹)、湿热痹阻证(热痹)两种不同证型的临床疗效.方法:选取80例类风湿关节炎患者,其中40例寒痹,40例热痹,随机分为对照组寒痹、对照组热痹、治疗组寒痹、治疗组热痹四组.对照组予以甲氨蝶呤片治疗,治疗组在对照组的基础上加用雷公藤片,12周为1个疗程.观察并记录四组患者治疗前后症状及体征、中医证候积分变化.结果:治疗后四组患者中医症状积分较前下降,治疗组总有效率(82.5％)高于对照组(62.5％),差异具有统计学意义(P＜0.05);四组患者各项指标较治疗前均有显著改善,治疗前后比较,差异有统计学意义(P＜0.05),其中治疗组热痹疗效显著(P＜0.01).结论:雷公藤片治疗类风湿关节炎可以改善临床症状及相关体征,其中对热痹疗效更明显,值得临床应用.

语种： 中文

作者： 陈沙;刘平安;张国民;刘慧萍;李玲

期刊： 科技资讯,2020年18(21):192-194+197 ISSN：1672-3791

作者机构： 湖南中医药大学医学基础教学实验中心 湖南长沙 410208;[刘慧萍; 张国民; 刘平安; 陈沙; 李玲] 湖南中医药大学

关键词： 智慧实验室;“5G”网络;实验教学;创新能力

摘要： "智慧实验室"概念的提出得益于"智慧地球"概念,在新一轮信息技术在高校普及的热潮席卷之际,智慧实验室很有可能会与"5G"网络的发展相伴行,借用"5G"网络的东风助力发展实验教学,在基本操作训练实验、综合设计性及研究创新性实验方面,发挥智慧化实验室的优势,调动学生对于实验课的主观能动性.积极参与实验设计,一定程度上减少验证性实验课给学生带来的"水课"的感受,增加实验教学的趣味性,使学生正视实验教学的意义,立志培养当今社会需要的具有创新能力的高素质人才.

语种： 中文

作者： 湛欢;周良荣;蔡冬华;彭玲;闫春晓;...

期刊： 中国卫生资源,2020年23(02):130-136 ISSN：1007-953X

作者机构： 湖南中医药大学人文与管理学院,长沙410208;湖南省卫生健康委员会,长沙410008;[王莹; 周良荣; 奉祁薇; 闫春晓; 湛欢; 钟锭; 李玲; 杜颖] 湖南中医药大学;[蔡冬华; 彭玲] 湖南省卫生健康委员会

关键词： 卫生费用核算体系2011;经常性卫生费用;卫生筹资;核算;湖南省

摘要： 目的 核算2017年湖南省的经常性卫生费用.方法 基于卫生费用核算体系2011,核算2017年湖南省经常性卫生费用的总量、筹资方案、机构流向、服务功能等.结果 2017年湖南省经常性卫生费用总量为1 791.94亿元,占国内生产总值的5.18％.治疗服务费用占比为70.55％,预防服务费用占比6.62％.经常性卫生费用中,家庭卫生支出比例为46.19％,居民负担仍然较重.结论 湖南省卫生筹资公平性有待提高,卫生筹资可持续性存在风险,卫生资金配置效率有待优化.

语种： 中文

作者： 陈沙;刘慧萍;李玲;李荣慧;黄姗姗

期刊： 长江丛刊,2020年(03):153+171 ISSN：2095-7483

作者机构： 湖南中医药大学

关键词： 实验教学;实验教师;教学团队

摘要： 高等院校发展根本在教学,教学基石在教师。实验教学与理论教学一样,在医学院校当中起着重要的承接作用。实验教师作为高校教学质量闭环保证的关键,其队伍建设需从职业发展规划、薪资待遇、教学团队意识3个方面进行考量,以此打造一批沉下心、坐得住、肯钻研的实验教师团体,提高实验教师对实验教学的兴趣,肯定实验教师在高校教师队伍中的地位,从而提高中医院校的软实力。

语种： 中文

作者： Zhang, Lulu;Liang, Enjun;Cheng, Yungchi;Mahmood, Tariq;Ge, Feng;...

期刊： Biomedicine & Pharmacotherapy,2020年128:110184 ISSN：0753-3322

通讯作者： Li, Li;Lu, Cheng;Tan, Y;Yi, JF

作者机构： [Bao, Mei; Li, Li; Lu, Cheng; Li, L; Liang, Enjun; Tan, Yong; Zhang, Lulu] China Acad Chinese Med Sci, Inst Basic Res Clin Med, Beijing 100700, Peoples R China.;[Bao, Mei; Yi, Jianfeng; Yi, JF; Zhang, Lulu] Yichun Univ, Key Lab Res Act Ingredients Nat Med Jiangxi Prov, Yichun 336000, Peoples R China.;[Zhou, Ke; Liang, Enjun] Hunan Univ Chinese Med, Affiliated Hosp 2, Changsha 410005, Peoples R China.;[Cheng, Yungchi] Yale Univ, Sch Med, Dept Pharmacol, New Haven, CT 06520 USA.;[Mahmood, Tariq] Quaid I Azam Univ, Fac Biol Sci, Dept Plant Sci, Islamabad, Pakistan.

通讯机构： [Lu, C; Li, L; Tan, Y ] C;[Yi, JF ] Y;China Acad Chinese Med Sci, Inst Basic Res Clin Med, Beijing 100700, Peoples R China.;Yichun Univ, Key Lab Res Act Ingredients Nat Med Jiangxi Prov, Yichun 336000, Peoples R China.

关键词： Combined medication;Natural medicine;Bacterial biofilm

摘要： Bacterial biofilms widely exist in nature and seriously threaten global public health. Biofilms always cause persistent infection and seriously aggravate the occurrence of antibiotic resistance, which makes the treatment of bacterial infection difficult. Current conventional therapies, such as antibiotics, bacteriophages and quorum sensing inhibitors, are widely used to combat biofilms. However, these therapies are inadequate for the safe and effective treatment of biofilms. Antibiotics often produce resistance in treated bacteria, and antibacterial peptides are easily decomposed by proteases, so their efficacy is reduced. These results indicate that the treatment of biofilms needs further improvement. Increasing evidence has shown that natural medicine therapies have significant inhibitory effects on biofilms. This review summarized and analyzed the efficacy characteristics and corresponding mechanisms of conventional and natural medicine therapies combatting biofilms. By comparison, the advantages and disadvantages of those therapies have been classified and interpreted, so we have inferred that combined medication with natural medicines will be a more effective strategy against biofilms. This review lays a promising foundation for the development of antibiofilm agents and provides novel thinking for the treatment of bacterial biofilm infections.

语种： 英文

作者： 谭传波;卢芳国;李玲;何谷良;吴涛;...

期刊： 中国油脂,2020年45(7):93-96 ISSN：1003-7969

作者机构： [周魁香; 黄闺; 赖琼玮; 谭传波] 湖南大三湘山茶油股份有限公司;[何谷良; 魏科; 卢芳国; 吴涛; 李玲] 湖南中医药大学

关键词： 山茶油;预防;胃肠炎;胃溃疡

摘要： 以不同工艺山茶油为研究对象,比较其对SD大鼠胃肠炎、胃溃疡的预防作用。结果表明:鲜榨山茶油、原香山茶油、精炼山茶油对大鼠胃肠炎、胃溃疡均有一定的预防作用,整体效应是原香山茶油、鲜榨山茶油优于精炼山茶油;在大鼠胃液白细胞数、胃和十二指肠病理变化的调节作用中,鲜榨山茶油又优于原香山茶油。

语种： 中文

作者： 宾东华;李玲;唐宇;王磊;罗丽云;...

期刊： 中华中医药杂志,2020年35(11):5775-5779 ISSN：1673-1727

作者机构： 湖南中医药大学第一附属医院;[唐宇; 何清湖; 李玲] 湖南中医药大学;[罗丽云; 王磊] 湖南东健药业有限公司;[宾东华; 周青] 中医药大学第一附属医院

关键词： 线粒体途径;解脲脲原体;知柏地黄汤;生精细胞;弱精子症

摘要： 目的:基于线粒体途径探讨知柏地黄汤含药血清干预解脲脲原体(UU)感染大鼠生精细胞活力的影响及其作用机制。方法:采用在体UU感染建模,随后原代提取大鼠生精细胞,以知柏地黄汤含药血清(低、中、高剂量组)为实验施加因素,同时设空白组、强力霉素组和模型组。给予相应药物干预后进行指标检测。结果:知柏地黄汤含药血清能改善UU感染的生精细胞活性,提高存活率(P<0.01, P<0.05);知柏地黄汤含药血清能有效提高UU感染的生精细胞线粒体ANT4转运活性(P<0.01,P<0.05);知柏地黄汤含药血清能有效上调UU感染的生精细胞中VDAC2、Cyp-D蛋白和mRNA表达水平(P<0.01, P<0.05),下调VDAC1蛋白和mRNA表达水平(P<0.01, P<0.05)。结论:知柏地黄汤可能通过抑制UU感染引起的VDAC1的过度激活,上调VDAC2、Cyp-D mRNA和蛋白表达,上调ANT4的转运活性,进而明显改善线粒体结构的破坏,最终改善生精细胞的活力。

语种： 中文

作者： 沈浮;付中应;吴泳蓉;李玲;赵昱东;...

期刊： 世界科学技术-中医药现代化,2020年22(03):622-631 ISSN：1674-3849

作者机构： 湖南中医药大学第一附属医院, 长沙, 410007;湖南中医药大学第二附属医院, 长沙, 410005;湖南中医药大学, 长沙, 410208;成都中医药大学, 成都, 610075;[赵昱东] 成都中医药大学

关键词： 金花清感颗粒;网络药理学;分子对接;人类新型冠状病毒3CL水解酶;ACE2受体;新型冠状病毒肺炎;机制

摘要： 目的 运用网络药理学及高通量分子对接技术,筛选金花清感颗粒中与人类新型冠状病毒3CL水解酶及ACE2受体蛋白结合活性较好的有效化合物,并对其干预COVID-19的机制进行预测.方法 本研究利用TCMSP数据库及高通量分子对接技术筛选金花清感颗粒中与人类新型冠状病毒3CL水解酶及ACE2受体对接活性较高的的活性化合物,并获取其对应靶标,利用STRING数据库进行靶标的蛋白网络互作,运用数据包进行京都基因与基因组百科全书(KEGG)通路富集分析,预测其作用机制.结果 金花清感颗粒共筛选有效活性化合物276种,涉及靶标基因325个.“药物-活性化合物-靶标”网络图中筛选出关键化合物11个,高通量分子对接筛选得到与人类新型冠状病毒3CL水解酶及ACE2受体结合活性较好的8个化合物,其靶基因的PPI网络主要涉及AKTI、HSP90AA1、RELA等核心靶蛋白.KEGG富集分析获得94条相关通路,主要包括卡波西肉瘤相关疱疹病毒感染、丙型肝炎、麻疹、人巨细胞病毒感染、EB病毒感染等病毒相关通路以及IL-17通路、TNF通路、AGE-RAGE等途径.结论 金花清感颗粒中3-甲氧基光甘草定、粗毛甘草素C、甘草查尔酮B等活性成分与人类新型冠状病毒3CL水解酶及ACE2受体酶结合活性较强,并且通过多种生物学途径及通路发挥干预COVID-19的作用.

语种： 中文

作者： Zhang, Xiao-Ling;Wang, Zhen-Zhen;Shao, Qian-Hang;Zhang, Zhao;Li, Lin;...

期刊： Brain Research Bulletin,2019年146:228-236 ISSN：0361-9230

通讯作者： Chen, NH;Zhang, Y

作者机构： [Wang, Zhen-Zhen; Shao, Qian-Hang; Zhang, Xiao-Ling; Chen, Nai-Hong; Zhang, Zhao] Chinese Acad Med Sci, State Key Lab Bioact Subst & Funct Nat Med, Inst Mat Med, Beijing 100050, Peoples R China.;[Wang, Zhen-Zhen; Shao, Qian-Hang; Zhang, Xiao-Ling; Chen, Nai-Hong; Zhang, Zhao] Chinese Acad Med Sci, Neurosci Ctr, Beijing 100050, Peoples R China.;[Wang, Zhen-Zhen; Shao, Qian-Hang; Zhang, Xiao-Ling; Chen, Nai-Hong; Zhang, Zhao; Chen, NH] Peking Union Med Coll, 1 Xian Nong Tan St, Beijing 100050, Peoples R China.;[Chen, Nai-Hong] Hunan Univ Chinese Med, Coll Pharm, Changsha 410208, Hunan, Peoples R China.;[Li, Lin] Capital Med Univ, Key Lab Neurodegenerat Dis, Minist Educ, Beijing 100053, Peoples R China.

通讯机构： [Chen, NH ] P;[Zhang, Y ] B;Peking Union Med Coll, 1 Xian Nong Tan St, Beijing 100050, Peoples R China.;Beijing Univ Chinese Med, Dept Anat, Sch Tradit Chinese Med, Beijing 100029, Peoples R China.;Chinese Acad Med Sci, Dept Pharmacol, Inst Mat Med, 1 Xian Nong Tan St, Beijing 100050, Peoples R China.

关键词： Akt;DJ-1;Mitochondrial dysfunction;Nuclear factor erythroid 2 (Nrf2);c-Jun N-terminal kinase (JNK)

摘要： Deletions or some mutations in the gene encoding the multifunctional protein, DJ-1, have been considered to be linked with autosomal recessive early onset Parkinson's disease (PD). Current emerging evidence suggests that DJ-1 is involved in the protection against oxidative stress-induced mitochondrial damage. However, the exact molecular mechanisms underlying this are not completely clear. The aim of this study was to investigate the effects of DJ-1 on the Akt pathway, nuclear factor erythroid 2-related factor (Nrf2), and c-Jun N-terminal kinase (JNK) with regard to modulating mitochondrial function. Here we showed that knockdown of DJ-1 resulted in mitochondrial dysfunction, including a decrease in active mitochondrial mass, complex I deficits, and inhibition of cellular adenosine 5′-triphosphate (ATP) content in the dopaminergic neuron-like cells PC12 and SH-SY5Y. Additionally, loss of DJ-1 impaired Akt signaling, and reduced nuclear translocation of Nrf2, thereby inhibiting activity of Nrf2-regulated downstream antioxidant enzymes such as heme oxygenase-1 and NAD(P)H quinone oxidoreductase 1. Moreover, DJ-1 knockdown also led to a significant increase in the mitochondrial reactive oxygen species, and then promoted the activation of JNK pathways. Furthermore, oxidative stress and mitochondrial dysfunction induced by knockdown of DJ-1 were blocked by a JNK inhibitor, which confirmed the important role of JNK activation in mitochondrial dysfunction. In conclusion, the present study indicates that DJ-1 knockdown leads to mitochondrial dysfunction in dopaminergic neuron-like cells, at least in part, through suppressing the Akt/GSK3β pathway and impairing the oxidative stress response, as well as through the subsequent increased JNK activation in dopaminergic neuron-like cells. © 2019 Elsevier Inc.

语种： 英文

作者： 李玲;刘慧萍;丁煌;何清湖

期刊： 科教导刊,2019年(5):171-172,190 ISSN：1674-6813

作者机构： 湖南中医药大学中医学院 湖南·长沙 410208;湖南中医药大学医学院 湖南·长沙 410208;[何清湖; 刘慧萍; 丁煌; 李玲] 湖南中医药大学

关键词： 课外科技活动;独立学院;创新能力

摘要： 目的:依托大学生创新训练中心,对独立学院学生开展课外科技活动,提升独立学院学生创新能力,有助于中医药院校独立学院学生适应当下社会对综合性高素质人才的需求。方法:依托大学生创新训练中心,结合二级学院的专业特点、分不同年级、根据兴趣双向选择,自主设计科研课题,通过参加实验设计、动手操作、记录分析和处理结果等综合评价中医药课外科技活动在提升独立学院学生创新能力的作用。结果:依托大学生创新训练中心的课外科技活动成果以论文为形式,在很大程度上提升了独立学院学生的创新能力,受益学生每学年约500人次。结论:依托一个具备较成熟的课外科技活动实施体系的大学生创新训练中心,开设一些高水平实验,在很大程度上能够提升独立学院学生创新能力。

语种： 中文

作者： Huang, Zhiqiang;Cheng, Chen;Liu, Zuwen;Luo, Wuhui;Zhong, Hong;...

期刊： Bioresource Technology,2019年275:421-424 ISSN：0960-8524

通讯作者： Luo, WH;Fu, W

作者机构： [Cheng, Chen; Luo, Wuhui; Liang, Changli; He, Guichun; Liu, Zuwen; Luo, WH; Huang, Zhiqiang] Jiangxi Univ Sci & Technol, Sch Resource & Environm Engn, Ganzhou 34100, Jiangxi, Peoples R China.;[Zhong, Hong] Cent S Univ, Sch Chem & Chem Engn, Changsha 410083, Hunan, Peoples R China.;[Li, Liqing] Jiangxi Univ Sci & Technol, Sch Met & Chem Engn, Ganzhou 34100, Jiangxi, Peoples R China.;[Deng, Lanqing] Hunan Univ Chinese Med, Sch Pharm, Changsha 410083, Hunan, Peoples R China.;[Fu, Weng] Univ Queensland, Sch Chem Engn, St Lucia, Qld 4072, Australia.

通讯机构： [Fu, W ] U;[Luo, WH ] J;Jiangxi Univ Sci & Technol, Sch Resource & Environm Engn, Ganzhou 34100, Jiangxi, Peoples R China.;Univ Queensland, Sch Chem Engn, St Lucia, Qld 4072, Australia.

关键词： Chlorella vulgaris;Flotation;Flotation efficiency;Gemini surfactant;Microalgae harvesting

摘要： Froth flotation has been proved to be a promising approach for commercial scale harvesting of microalgae. However, all the surfactants used in the microalgae flotation harvesting process are conventional monomeric surfactants contain a single similar hydrophobic group in the molecule, which results in a low harvesting efficiency. In this work, a novel Gemini surfactant, N,N′-bis(cetyldimethyl)-1,4-butane diammonium dibromide (BCBD) was prepared, and originally recommended as a collector for froth flotation harvesting of Chlorella vulgaris from culture medium. The performance of BCBD was compared with the results acquired using its conventional monomeric surfactant cetyl trimethyl ammonium bromide (CTAB). The bench-scale flotation results showed that BCBD had excellent collecting power for Chlorella vulgaris. Achieving the obviously superior flotation harvesting performance (flotation recovery increased by 21.4% and enrichment ratio increased by 22.9), the dosage of Gemini type BCBD collector is five times less than that of monomeric CTAB collector. © 2018 Elsevier Ltd

语种： 英文

作者： 张世鹰;何谷良;卢芳国;李玲;张波;...

期刊： 中华中医药杂志,2019年34(3):1188-1193 ISSN：1673-1727

作者机构： 湖南中医药大学研究生院;[张世鹰] 江西省人民医院;湖南中医药大学医学院;湖南中医药大学中医学院;湖南中医药大学第一附属医院

关键词： 流感病毒;Toll样受体;麻杏石甘汤;煎煮;先煎

摘要： 目的:基于TL R7/8介导的I F N-α/β蛋白表达水平探讨麻黄先煎之麻杏石甘汤抗流感病毒效应机制。方法:以TLR7/8激活剂(R848)、抑制剂(抗体TLR7和抗体TLR8)干预的小鼠巨噬细胞为对照,以酶联免疫吸附技术检测流感病毒干预小鼠巨噬细胞24、48h的IFN-α/β分泌水平。以流感病毒干预的小鼠巨噬细胞为对照,以酶联免疫吸附技术和Western Blot技术检测麻黄先煎之麻杏石甘汤含药血清干预24、48h的流感病毒感染的小鼠巨噬细胞IFN-α/β分泌水平和蛋白表达水平。结果:与正常大鼠血清组及胎牛血清组比较,TLR7/8激活剂(R848)与流感病毒干预24、48h的RAW264.7细胞IFN-α和IFN-β分泌水平和蛋白表达水平显著升高(P<0.05);与病毒干预组比较,四药同煎及麻黄先煎20、25、30min之麻杏石甘汤含药血清作用24、48h后,流感病毒感染后的巨噬细胞IFN-α/β的分泌水平和蛋白表达水平显著降低(P<0.05);与四药同煎组比较,在含药血清作用48h的时间点,麻黄先煎25min之麻杏石甘汤组IFN-α分泌水平显著降低(P<0.05)。结论:流感病毒感染对TLR7/8介导的IFN-α/β蛋白分泌水平有明显的上调作用,麻杏石甘汤能够下调流感病毒感染的巨噬细胞IFN-α/β分泌水平和蛋白表达水平,在作用48h时间点,麻黄先煎25min之麻杏石甘汤含药血清对IFN-α分泌水平的调节作用优于四药同煎。

语种： 中文

作者： Yang, Shaofeng;Li, Linghui;Zhu, Liguo;Zhang, Chao;Li, Zhaoyong;...

期刊： JOURNAL OF CELLULAR PHYSIOLOGY,2019年234(8):13639-13648 ISSN：0021-9541

通讯作者： Zhang, C;Zhu, LG

作者机构： [Yang, Shaofeng; Zhang, Chao; Zhang, C; Li, Zhaoyong; Nie, Ying; Luo, Zhenhua; Guo, Yantao] Hunan Univ Chinese Med, Hosp 1, Dept Spine, 95 Shaoshan Middle St, Changsha 410007, Hunan, Peoples R China.;[Li, Linghui; Zhu, Liguo] China Acad Chinese Med Sci, Wangjing Hosp, Dept Gen Orthoped, Huajiadi St, Beijing 100102, Chaoyang Distri, Peoples R China.

通讯机构： [Zhang, C ] H;[Zhu, LG ] C;Hunan Univ Chinese Med, Hosp 1, Dept Spine, 95 Shaoshan Middle St, Changsha 410007, Hunan, Peoples R China.;China Acad Chinese Med Sci, Wangjing Hosp, Dept Gen Orthoped, Huajiadi St, Beijing 100102, Chaoyang Distri, Peoples R China.

关键词： aggrecanase 2;aucubin;collagen type 2;cyclic AMP responsive element binding protein;interleukin 1beta;microRNA;microRNA 140 5p;stromelysin;tumor necrosis factor;unclassified drug;aucubin;CREB1 protein, human;cyclic AMP responsive element binding protein;IL1B protein, human;interleukin 1beta;iridoid;microRNA;Mirn140 microRNA, human;tumor necrosis factor;adolescent;adult;Article;cell differentiation;controlled study;enzymatic degradation;extracellular matrix;gene function;gene identification;gene knockdown;gene targeting;genetic transfection;human;human cell;human tissue;intervertebral disk degeneration;nucleus pulposus;pathogenesis;priority journal;protein function;signal transduction;cell culture;drug effect;extracellular matrix;female;intervertebral disk degeneration;male;metabolism;middle aged;nucleus pulposus;pathology;young adult;Adolescent;Adult;Cells, Cultured;Cyclic AMP Response Element-Binding Protein;Extracellular Matrix;Female;Humans;Interleukin-1beta;Intervertebral Disc Degeneration;Iridoid Glucosides;Male;MicroRNAs;Middle Aged;Nucleus Pulposus;Signal Transduction;Tumor Necrosis Factor-alpha;Young Adult

摘要： In intervertebral disc degeneration (IDD), increased proinflammatory molecules secreted by human nucleus pulposus cells (HNPCs) could promote the expression of extracellular matrix (ECM)-degrading enzymes. IDD could be affected by both genetic and environmental factors, including microRNAs (miRNAs). Aucubin, the active ingredient of a traditional Chinese medicine herb Du Zhong, has been reported to promote osteogenic differentiation; however, the role of aucubin in IDD and the underlying mechanism remain unclear. Herein, we evaluated the effect of aucubin on TNF-α- or IL-1β-induced ECM degradation in HNPCs. By using online tools, miR-140 was selected as a candidate miRNA that is related to TNF-α or IL-1β signaling. Overexpression of miR-140 enhanced the effect of aucubin on ECM degradation. Moreover, cAMP responsive element binding protein 1 (CREB1), a major transcriptional factor in immune-related signaling, was a direct downstream target of miR-140. CREB1 knockdown mimicked the function of miR-140 overexpression on ECM degradation. In summary, aucubin might ameliorate IL-1β- or TNF-α-induced ECM degradation in HNPCs through regulating miR-140/CREB1. © 2019 Wiley Periodicals, Inc.

语种： 英文

作者： Ning, Y.;Lu, F.;Li, L.;Wei, K.;Hu, J.;...

作者机构： [Lu, F.; Wei, K.; Ning, Y.; Hu, J.; Li, L.] Hunan Univ Chinese Med, Changsha, Hunan, Peoples R China.;[He, Q.] Changsha Med Univ, Changsha, Hunan, Peoples R China.

会议名称： 17th International Congress of Immunology (IUIS)

会议时间： OCT 19-23, 2019

会议地点： Beijing, PEOPLES R CHINA

语种： 英文

作者： Huang, Zhiqiang;Cheng, Chen;Zhong, Hong;Li, Liqing;Guo, Zhizhong;...

期刊： Minerals Engineering,2019年132:22-26 ISSN：0892-6875

通讯作者： Li, LQ;Fu, W

作者机构： [Cheng, Chen; Guo, Zhizhong; He, Guichun; Yu, Xinyang; Huang, Zhiqiang] Jiangxi Univ Sci & Technol, Sch Resource & Environm Engn, Ganzhou 34100, Jiangxi, Peoples R China.;[Zhong, Hong] Cent S Univ, Sch Chem & Chem Engn, Changsha 410083, Hunan, Peoples R China.;[Han, Haijun; Li, Liqing; Li, LQ] Jiangxi Univ Sci & Technol, Sch Met & Chem Engn, Ganzhou 34100, Jiangxi, Peoples R China.;[Deng, Lanqing] Hunan Univ Chinese Med, Sch Pharm, Changsha 410083, Hunan, Peoples R China.;[Fu, Weng] Univ Queensland, Sch Chem Engn, St Lucia, Qld 4072, Australia.

通讯机构： [Fu, W ] U;[Li, LQ ] J;Jiangxi Univ Sci & Technol, Sch Met & Chem Engn, Ganzhou 34100, Jiangxi, Peoples R China.;Univ Queensland, Sch Chem Engn, St Lucia, Qld 4072, Australia.

关键词： Chloride minerals;Desalination;Efficiency;Flotation;Ore treatment;Potash;Potassium chloride;Potassium fertilizers;Sodium chloride;Surface active agents;Agricultural yields;Flotation collectors;Flotation separation;Froth flotation process;Gemini surfactant;Hydrophobic groups;Monomeric surfactants;Production efficiency;Froth flotation

摘要： Potash fertilizer ensures the necessary agricultural yields to feed an increasing world population. Over 80% of the world's potash fertilizer is produced by the selective froth flotation of KCl (sylvite) from NaCl (halite). However, all the surfactants used in the potash froth flotation process are traditional monomeric surfactants contain a single similar hydrophobic group in the molecule, which leads to a low production efficiency. In this work, a novel Gemini surfactant, N,N’-bis (octadecyldimethyl)-1,4-butane diammonium dibromide (BOBD) was prepared, and originally recommended as a collector for froth flotation separation of sylvite from halite for potash ore. The performance of BOBD was compared with the results acquired using its traditional monomeric surfactant octadecylamine hydrochloride (OAH). The bench-scale flotation results showed that BOBD had excellent collecting power for sylvite and significant selectivity against halite at natural pH. Moreover, BOBD presented stronger desalination efficiency than OAH. The results gained with the Gemini collector were better in terms of KCl recovery and NaCl content than those gained with four times higher dosages of the conventional monomeric surfactant. © 2018 Elsevier Ltd

语种： 英文

作者： Wei, K.;Zhou, J. -H.;Chen, Y. -C.;Sun, M. -J.;Dai, X. -W.;...

作者机构： [Lu, F.; Wei, K.; Ning, Y.; Chen, Y. -C.; Hu, J.; Xiang, Q.; Zhou, J. -H.; Li, L.] Hunan Univ Chinese Med, Changsha, Hunan, Peoples R China.;[Sun, M. -J.; Dai, X. -W.] Hunan Busky Pharmaceut Co Ltd, Changsha, Hunan, Peoples R China.

会议名称： 17th International Congress of Immunology (IUIS)

会议时间： OCT 19-23, 2019

会议地点： Beijing, PEOPLES R CHINA

语种： 英文

作者： 尹显楼;詹济华;谭洋;裴刚;李玲

期刊： 食品与机械,2019年35(6):172-176 ISSN：1003-5788

作者机构： 湖南中医药大学药学院,湖南 长沙,410208;衡阳市中心医院药剂科,湖南 衡阳,421001;[詹济华] 衡阳市中心医院;[尹显楼; 谭洋; 李玲; 裴刚] 湖南中医药大学

关键词： 铁苋菜;极性;萃取物;抗氧化;抑菌活性

摘要： 采用1,1-二苯基-2-三硝基苯肼(DPPH)法、β-胡萝卜素漂白法和清除一氧化氮自由基(NO)法来测定不同极性铁苋菜萃取物的抗氧化活性;通过微孔比浊法比较铁苋菜萃取物对3种革兰氏阴性细菌(大肠杆菌、铜绿假单胞菌和幽门螺杆菌)和4种革兰氏阳性细菌(表皮葡萄球菌、枯草芽孢杆菌、藤黄微球菌和金黄色葡萄球菌)的抑菌效果.结果表明,铁苋菜不同极性萃取物均有较好的清除DPPH、NO自由基和抑制β-胡萝卜素漂白的效果,浓度为2.0 mg/mL时,正丁醇萃取物清除DPPH自由基和抑制β-胡萝卜素漂白能力最佳,DPPH自由基清除率为94.60％,β-胡萝卜素抑制率为83.15％,乙酸乙酯萃取物清除NO自由基能力最强,清除率为82.72％.抑菌试验结果显示,铁苋菜各萃取物均有较好抑菌效果,对革兰氏阳性菌抑菌效果优于革兰氏阴性菌,其中石油醚萃取物对大肠杆菌、表皮葡萄球菌和金黄色葡萄球菌抑制作用最佳,MIC值分别为0.625,0.315,1.250 mg/mL.铁苋菜萃取物具有较好的抗氧化及抑菌效果.

语种： 中文

作者： 何谷良;袁娉;黄家望;廖灿;宁毅;...

期刊： 时珍国医国药,2019年30(4):788-790 ISSN：1008-0805

作者机构： 湖南中医药大学医学院, 湖南, 长沙, 410208;湖南中医药大学中西结合学院, 湖南, 长沙, 410208;湖南中医药大学中医学院, 湖南, 长沙, 410208;[何谷良; 宁毅; 陈伶利; 黄家望; 袁娉; 卢芳国; 廖灿; 李玲] 湖南中医药大学

关键词： 黄芩苷;耐甲氧西林金黄色葡萄球菌;生物膜

摘要： 目的研究从中药黄芩干燥根中提取分离药效成分黄芩苷体外对耐甲氧西林金黄色葡萄球菌活性的影响.方法以琼脂扩散法、液体稀释微孔板法检测黄芩苷对MRSA的抑菌环大小、最低抑菌浓度(MIC)和最低杀菌浓度(MBC) ;以96孔酶标板结晶紫法检测黄芩苷对MRSA的最小生物膜清除浓度(MBEC) .结果与DMSO比较,黄芩苷与氯霉素(阳性对照药物)对MRSA的抑菌环半径显著增加(P < 0. 01) ,与氯霉素比较,黄芩苷对MRSA的抑菌环半径显著缩小(P < 0. 05) ;黄芩苷对MRSA的MIC为0. 5mg /ml; MBC为1mg /ml;黄芩苷对MRSA的MBEC为0. 5mg /ml.结论黄芩苷对MRSA有一定的抗菌、抑菌作用,其效应机制可能与其抑制细菌生物膜的形成有关.

语种： 中文