期刊：
Evidence-Based Complementary and Alternative Medicine,2022年2022 ISSN：1741-427X
通讯作者：
Hu, R.
作者机构：
[Hu, Ruicheng; Wang, Lile] Hunan Normal Univ, Hunan Prov Peoples Hosp, Dept Resp Med, Affiliated Hosp 1, Changsha 410016, Peoples R China.;[Dai, Aiguo] Hunan Univ Chinese Med, Med Sch, Dept Resp Dis, Changsha 410208, Peoples R China.
通讯机构：
Department of Respiratory Medicine, Hunan Provincial People's Hospital, The First-affiliated Hospital of Hunan Normal University, Changsha, China
摘要：
<i>Objective</i>. Non-small-cell lung cancer (NSCLC) is one of the most lethal cancers. Although cisplatin-based chemotherapies have been regarded as a promising treatment approach, cisplatin resistance still remains one of the major clinical challenges. Curcumin, a naturally occurring polyphenol, has been proved to increase chemotherapeutic efficiency of NSCLC cells. However, the role of curcumin in cisplatin-resistant NSCLC cells has been rarely investigated. This study aims to investigate whether curcumin enhances cisplatin sensitivity of human NSCLC cells and its underlying mechanisms. <i>Method</i>. A549/DDP and H1299/DDP cells were treated by DDP or/and curcumin before cell viability, and apoptosis were determined by using a CCK-8 assay and flow cytometer. The expressions of apoptosis and ER stress-related proteins, including cleaved caspase-3, cleaved PARP, CHOP, GRP78, XBP-1, ATF6, and caspase-4, were measured by the qPCR and western blotting. After cotreatment by DDP and curcumin, A549/DDP and H1299/DDP cells were further treated by the ER stress inhibitor, salubrinal (20 <i>μ</i>m), after which the cell apoptosis and viability were detected. <i>Result</i>. Treatment by DDP and curcumin can substantially decrease cell viability, while can increase the cell apoptosis rate, elevate mRNA and protein expressions of apoptosis and ER stress-related proteins, compared with cells treated by DDP or curcumin alone. Salubrinal treatment can counteract the suppressive effect of DDP and curcumin on cell viability and decrease the cell apoptosis of A549/DDP and H1299/DDP cells. <i>Conclusion</i>. Curcumin can increase the sensitivity of NSCLC to cisplatin through an ER stress pathway and thus can be served as one of the molecular targets for overcoming the cisplatin resistance.
作者：
Liu, Yong-Ping;Lei, Jian;Yin, Ming-Ming;Chen, Yi
期刊：
Anti-Cancer Agents in Medicinal Chemistry,2022年22(13):2448-2457 ISSN：1871-5206
通讯作者：
Liu, Y.-P.
作者机构：
[Chen, Yi; Liu, Yong-Ping] Hunan Univ Chinese Med, Sch Med, Dept Physiol, 300 Xueshi Rd,Hanpu Sci & Educ Pk, Changsha 410208, Hunan, Peoples R China.;[Lei, Jian] Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Hunan, Peoples R China.;[Yin, Ming-Ming] Hunan Univ Chinese Med, Coll Integrated Tradit Chinese & Western Med, Changsha 410208, Hunan, Peoples R China.
通讯机构：
Department of Physiology, School of Medicine, Hunan University of Chinese Medicine, 300 Xueshi Road, Hanpu Science and Education Park, Yuelu District Hunan, Changsha, China
关键词：
Metal complex;necroptosis inducer;anti-cancer;triple-negative breast cancer;ROS;oxidative stress.
摘要：
Aim: This study aimed to investigate the anticancer effect and the underlying mechanisms of organoantimony (III) fluoride on MDA-MB-231 human breast cancer cells. Methods: Five cancer and one normal cell line were treated with an organoantimony (III) compound 6-cyclohexyl-12- fluoro-5,6,7,12-tetrahydrodibenzo[c,f][1,5]azastibocine (denoted as C4). The cell viability was detected by MTT assay. Induction of cell death was determined by Hoechst 33342/PI staining and Annexin-V/PI staining. The effect of C4 on the necroptotic relative protein was determined by Western blot analysis. Results: Among the five cancer cell lines, C4 decreased the viability of MDA-MB-231, MCF-7 and A2780/cisR, and showed less toxicity on normal human embryonic kidney cells. In breast cancer cell line MDA-MB-231, the C4 treatment induced necrotic cell death as well as LDH release in a time- and dose-dependent manner. Moreover, C4 could increase the expression of phosphorylated RIPK3 and MLKL proteins. Overall, the C4 treatment resulted in the reduction of mitochondrial transmembrane potential and accumulation of ROS in MDA-MB-231 cells. Conclusion: C4-induced necroptosis could be ascribed to glutathione depletion and ROS elevation in MDA-MB-231 cells. Our findings illustrate C4 to be a potential necroptosis inducer for breast cancer treatment.
摘要：
The circadian rhythm is an endogenous clock system that coordinates and optimizes various physiological and pathophysiological processes, which accord with the master and the peripheral clock. Increasing evidence indicates that endogenous circadian rhythm disruption is involved in the lesion volume and recovery of ischemic stroke. As a critical recovery mechanism in post-stroke, angiogenesis reestablishes the regional blood supply and enhances cognitive and behavioral abilities, which is mainly composed of the following processes: endothelial cell proliferation, migration, and pericyte recruitment. The available evidence revealed that the circadian governs many aspects of angiogenesis. This study reviews the mechanism by which circadian rhythms regulate the process of angiogenesis and its contribution to functional recovery in post-stroke at the aspects of the molecular level. A comprehensive understanding of the circadian clock regulating angiogenesis in post-stroke is expected to develop new strategies for the treatment of cerebral infarction.
作者机构：
[Chen, Keke; Zhou, Ji; Dong, Jun; He, Qizhi; Chen, Danna; Ma, Shuheng; Wang, Jingya] Changsha Med Univ, Academician Workstn, Changsha 410219, Hunan, Peoples R China.;[Ning, Yi] Hunan Univ Chinese Med, Dept Microbiol, Med Sch, Changsha 410208, Hunan, Peoples R China.;[Zhou, Ji; He, Qizhi; Chen, Danna] Changsha Med Univ, Sch Basic Med Sci, Changsha, Hunan, Peoples R China.;[Dong, Jun; He, Qizhi; Ma, Shuheng] Changsha Med Univ, Discipline Basic Med Applicat, Changsha, Hunan, Peoples R China.
通讯机构：
[Danna Chen] A;[Yi Ning] D;Academician Workstation, Changsha Medical University, Changsha, Hunan, People’s Republic of China<&wdkj&>School of Basic Medical Science, Changsha Medical University, Changsha, Hunan, People’s Republic of China<&wdkj&>Department of Microbiology, The Medicine School of Hunan University of Chinese Medicine, Changsha, Hunan, People’s Republic of China
期刊：
Drug Delivery,2022年29(1):1675-1683 ISSN：1071-7544
通讯作者：
Fangguo Lu
作者机构：
[Chen, Pingan; Ning, Yi; Wang, Xiaoqi; Lu, Fangguo; Hu, Jue; Xiao, Rong; Liu, Shiwu] Hunan Univ Chinese Med, Med Sch, Dept Microbiol, Changsha 410208, Hunan, Peoples R China.;[Li, Ling] Hunan Univ Chinese Med, Chinese Med Sch, Expt Ctr Mol Biol, Changsha, Hunan, Peoples R China.
通讯机构：
[Fangguo Lu] D;Department of Microbiology, The Medicine School of Hunan University of Chinese Medicine, Changsha, Hunan, People’s Republic of China
期刊：
Computational and Mathematical Methods in Medicine,2022年2022 ISSN：1748-6718
通讯作者：
Tian, X.
作者机构：
[Tian, Xuefei; Tian, Sha] Hunan Univ Chinese Med, Coll Integrated Chinese & Western Med, Dept Internal Med, Changsha, Peoples R China.;[Guo, Yinmei] Hunan Univ Chinese Med, Hunan Key Lab Tradit Chinese Med Prescript & Synd, Changsha, Peoples R China.;[Fu, Jiajun] Med Sch Hunan Univ Chinese Med, Changsha, Peoples R China.;[Li, Zijing] Hunan Univ Chinese Med, Acupuncture Moxibust Tuina & Rehabil, Changsha, Peoples R China.;[Li, Jing] First Hosp Hunan Univ Chinese Med, Dept Oncol, Changsha, Peoples R China.
通讯机构：
Department Of Internal Medicine, College Of Integrated Chinese And Western Medicine, Hunan University Of Chinese Medicine, China
摘要：
<i>Objective</i>. Bioinformatics methods were used to analyze non-small-cell lung cancer gene chip data, screen differentially expressed genes (DEGs), explore biomarkers related to NSCLC prognosis, provide new targets for the treatment of NSCLC, and build immunotyping and line-map model. <i>Methods</i>. NSCLC-related gene chip data were downloaded from the GEO database, and the common DEGs of the two datasets were screened by using the GEO2R tool and FunRich 3.1.3 software. DAVID database was used for GO analysis and KEGG analysis of DEGs, and protein-protein interaction (PPI) network was constructed by STRING database and Cytoscape 3.8.0 software, and the top 20 hub genes were analyzed and screened out. The expression of pivot genes and their relationship with prognosis were verified by multiple external databases. <i>Results</i>. 159 common DEGs were screened from the two datasets. PPI network was constructed and analyzed, and the genes with the top 20 connectivity were selected as the pivotal genes of this study. The results of survival analysis and the patients’ survival curve was reflected in the line graph model of NSCLC. <i>Conclusion</i>. Through the screening and identification of the VIM-AS1 gene, as well as the analysis of immune infiltration and immune typing, the successful establishment of the rosette model has a certain guiding value for the molecular targeted therapy of patients with non-small-cell lung cancer.
作者机构：
[肖荣; 熊涛; 王平; 陈纯静; 赵澄; 张香港; 胡珏] Medical College, Hunan University of Traditional Chinese Medicine, Changsha, 410208, China;The First Affiliated Hospital, Hunan University of Traditional Chinese Medicine, Changsha, 410208, China;[卢芳国] Medical College, Hunan University of Traditional Chinese Medicine, Changsha, 410208, China, The First Affiliated Hospital, Hunan University of Traditional Chinese Medicine, Changsha, 410208, China
通讯机构：
[Lu, F.-G.] M;[Lu, F.-G.] T;Medical College, China;The First Affiliated Hospital, China
