摘要：
BACKGROUND: Herbal medicine plays an important role in health, particularly in remote parts of developing areas with few health facilities. According to WHO estimates, about three-quarters of the world's population currently use herbs or traditional medicines to treat various ailments, including liver diseases. Several studies have found that the use of medicinal plants was effective in the treatment of infectious and non-infectious diseases. Hepatitis and liver cirrhosis associated with many clinical manifestations can be treated with allopathic medicines, but reports of a number of side effects including immunosuppression, bone marrow suppression, and renal complications have motivated researchers to explore more natural herbal medicines with low or no side effects and with high efficacy in treating hepatic diseases. METHODS: Databases including PubMed, Medline, and Google Scholar were searched for findings on the hepatoprotective effects of plants. RESULTS: Various medicinal plants are used for the treatment of liver disorders. The range of alternative therapies is huge, and they are used worldwide, either as part of primary health care or in combination with conventional medicine. Hepatoprotective plants contain a variety of chemical constituents including flavonoids, alkaloids, glycosides, carotenoids, coumarins, phenols, essential oil, organic acids, monoterpenes, xanthenes, lignans, and lipids. CONCLUSION: This review shows that numerous plants are found to contain hepatoprotective compounds. However, further studies are needed to determine their association with existing regimes of antiviral medicines and to develop evidence-based alternative medicine to cure different kinds of liver disease in humans.
作者机构：
[Liu, Bin; Dang, Wenya; Zhou, Hongyan; Tong, Chunyi] Hunan Univ, Coll Biol, Changsha 410082, Hunan, Peoples R China.;[Liu, Bin; Yang, Yupei; Qin, Yan; Li, Bin; Wang, Wei; Liu, Yongbei] Hunan Univ Chinese Med, Sch Pharm, Innovat Mat Med Res Inst, TCM & Ethnomed Innovat & Dev Int Lab, Changsha 410208, Hunan, Peoples R China.;[Zou, Wei] Hunan Prov Maternal & Child Hlth Care Hosp, NHC Key Lab Birth Defects Res Prevent & Treatment, Changsha 410008, Hunan, Peoples R China.
通讯机构：
[Liu, Bin; Liu, B; Wang, W] H;Hunan Univ, Coll Biol, Changsha 410082, Hunan, Peoples R China.;Hunan Univ Chinese Med, Sch Pharm, Innovat Mat Med Res Inst, TCM & Ethnomed Innovat & Dev Int Lab, Changsha 410208, Hunan, Peoples R China.
摘要：
T4 polynucleotide kinase (PNK) is the primary member of the 5'-kinase family that can transfer the gamma-phosphate residue of ATP to the 5'-hydroxyl group of oligonucleotides. In this article, using the differential quenching ability of reduced graphene oxide (rGO) towards the fluorophore-labeled DNA probe, we propose a novel method for detecting T4 PNK activity assisted by ligase reaction. Under the optimized conditions, the detection limit of T4 PNK was estimated to be 0.0002 U muL(-1) in the linear region of 0.001 U muL(-1)-0.1 U muL(-1). Additionally, the developed method was used to screen regulators of T4 PNK from natural compounds. The compound f isolated from the root of Kadsura coccinea (Lem.) A.C. Smith was found to stimulate T4 PNK activity in a concentration-dependent manner in vitro. Finally, the method was used to monitor the relation of T4 PNK activity with pelvic inflammatory disease (PID). The results demonstrated that the development of this disease could inhibit T4 PNK activity to some extent. In summary, the above data indicate that the method not only provides a universal platform for monitoring T4 PNK activity, but also shows great potential to be used in drug screening and clinic diagnosis.
作者机构：
[Yu Huanghe; Zeng Rong; Tasneem, Shumaila; Qiu Yi-xing; Li Bin; Wang Wei] Hunan Univ Chinese Med, TCM & Ethnomed Innovat & Dev Int Lab, Sch Pharm, Changsha 410208, Hunan, Peoples R China;[Yu Huanghe; Zeng Rong; Tasneem, Shumaila; Qiu Yi-xing; Li Bin; Wang Wei] Hunan Univ Chinese Med, Innovat Mat Med Res Inst, Sch Pharm, Changsha 410208, Hunan, Peoples R China;[Yu Huanghe; Lin Ye; Li Xin; Zhen, Yang; Wang Yu-hong; Cai Xiong] Hunan Univ Chinese Med, Inst Innovat & Appl Res Chinese Med, Changsha 410208, Hunan, Peoples R China
通讯机构：
[Wang Wei; Cai Xiong] H;Hunan Univ Chinese Med, TCM & Ethnomed Innovat & Dev Int Lab, Sch Pharm, Changsha 410208, Hunan, Peoples R China. Hunan Univ Chinese Med, Innovat Mat Med Res Inst, Sch Pharm, Changsha 410208, Hunan, Peoples R China. Hunan Univ Chinese Med, Inst Innovat & Appl Res Chinese Med, Changsha 410208, Hunan, Peoples R China.
摘要：
BACKGROUND: Rheumatoid arthritis (RA) is a chronic autoimmune diseased state, characterized by hyperplasia of the synovial membrane, degradation of cartilage, and bone erosion of diarthrodial joints. Kadsura heteroclita (Roxb) Craib (Schizandraceae), a traditional Tujia ethnomedicine called Xue Tong in China, has been long used for the prevention and treatment of rheumatic and arthritic diseases, especially in the southern China. This study aimed to evaluate anti-arthritic effects of the ethanol extract of Kadsura heteroclita stems (KHS) on complete Freund's adjuvant (CFA)-induced arthritis (AIA) in rats, as well as to explore the underlying mechanisms of anti-arthritis. METHODS: AIA was established in male Sprague-Dawley (SD) rats as described previously, and animals were daily treated by gavage with KHS ethanol extract (200, 400, or 800mg/kg) or vehicle (0.3% CMCNa) throughout the 30-day experiment. The incidence and severity of arthritis were evaluated using clinical parameters. At the end of experiments, tissue swelling and bone destruction of the hind paws were assessed by computed tomography (CT) and histopathological analyses. Serum levels of tumor necrosis factor (TNF-alpha), interleukin-1beta (IL-1beta), IL-6, and IL-17A and IL-17F were measured by ELISA, and protein expression of matrix metalloproteinases-1 (MMP-1), MMP-3 and tissue inhibitor of MMP-1 (TIMP-1) were detected by Western blot. RESULTS: Treatment with KHS dose-dependently inhibited paw swelling and reduced arthritis scores of AIA rats. CT images displayed that KHS remarkably protected AIA rats from tissue swelling and bone erosion of joints. Histopathological analyses revealed that KHS markedly reduced inflammatory cell infiltration, synovial proliferation, and the formation of pannus in the ankle joints of AIA rats. KHS was found to significantly suppress the production of TNF-alpha, IL-1 beta, IL-6, IL-17A and IL-17F, inhibited the protein expression of MMP-1 and MMP-3, and elevated the protein expressions of TIMP-1. CONCLUSION: KHS demonstrates potential anti-arthritic effects via inhibiting pivotal mediators of inflammation and cartilage destruction. This study strongly supports identification and isolation of active fractions of KHS which would be a potential candidate for further investigation as a new anti-arthritic botanical drug.
期刊：
Mediators of Inflammation,2019年2019:6453296 ISSN：0962-9351
通讯作者：
Xing, Wei;Xiao, Xian-Zhong
作者机构：
[Peng, Yue; Xing, Wei; Yang, Ming-Shi; Wang, Qian-Lu; Gao, Min] Cent South Univ, Dept Intens Care Med, Xiangya Hosp 3, Changsha 410013, Hunan, Peoples R China.;[Yang, Lei] Hunan Univ Chinese Med, Hosp 1, Dept Pharm, Changsha 410003, Hunan, Peoples R China.;[Xiao, Xian-Zhong] Cent South Univ, Dept Pathophysiol, Xiangya Sch Med, Changsha 410078, Hunan, Peoples R China.
通讯机构：
[Xing, Wei; Xiao, Xian-Zhong] C;Cent South Univ, Dept Intens Care Med, Xiangya Hosp 3, Changsha 410013, Hunan, Peoples R China.;Cent South Univ, Dept Pathophysiol, Xiangya Sch Med, Changsha 410078, Hunan, Peoples R China.
摘要：
Objectives: To investigate the protective effect of ginsenoside Rg1 on relieving sepsis-induced lung inflammation and injury in vivo and in vitro. Methods: Cultured human pulmonary epithelial cell line A549 was challenged with LPS to induce cell injury, and CLP mouse model was generated to mimic clinical condition of systemic sepsis. Rg1 was applied to cells or animals at indicated dosage. Apoptosis of cultured cells was quantified by flow cytometry, along with ELISA for inflammatory cytokines in supernatant. For septic mice, lung tissue pathology was examined, plus ELISA assay for serum cytokines. Western blotting was used to examine the activation of inflammatory pathways and ER stress marker proteins in both cells and mouse lung tissues. Reactive oxygen species (ROS) level was quantified by DCFDA kit. Results: Ginsenoside Rg1 treatment remarkably suppressed apoptosis rate of LPS-induced A549 cells, relieved mouse lung tissue damage, and elevated survival rate. Rg1 treatment also rescued cells from LPS-induced intracellular ROS. In both A549 cells and mouse lung tissues, further study showed that Rg1 perfusion significantly suppressed the secretion of inflammatory cytokines including tumor necrosis factor- (TNF-) alpha and interleukin- (IL-) 6 and relieved cells from ER stress as supported by decreased expression of marker proteins via upregulating sirtuin 1 (SIRT1). Conclusion: Our results showed that ginsenoside Rg1 treatment effectively relieved sepsis-induced lung injury in vitro and in vivo, mainly via upregulating SIRT1 to relieve ER stress and inflammation. These findings provide new insights for unrevealing potential candidate for severe sepsis accompanied with lung injury.
作者机构：
[杜可] Department of Pharmacology, School of Pharmacy, Hunan University of Chinese Medicine, Changsha, 410208, China;[廖端芳] Division of Stem Cell Regulation and Application, Hunan University of Chinese Medicine, Changsha, 410208, China;[张婵娟; Qin L.; 石雅宁] Department of Pharmacology, School of Pharmacy, Hunan University of Chinese Medicine, Changsha, 410208, China, Division of Stem Cell Regulation and Application, Hunan University of Chinese Medicine, Changsha, 410208, China
通讯机构：
[Qin, Li] D;Division of Stem Cell Regulation and Application, Hunan University of Chinese Medicine, Changsha 410208, China.
作者机构：
[Guo, Qiuyan; Zhang, Yanqiong; Yang, Hongjun; Xu, Haiyu; Li, Weijie; Mao, Xia; Wang, Ping; Su, Jin] China Acad Chinese Med Sci, Inst Chinese Mat Med, 16 Nanxiaojie, Beijing 100700, Peoples R China.;[Li, Sen] Hunan Univ Chinese Med, Dept Pharmaceut, Changsha 410208, Hunan, Peoples R China.;[Guo, Rui; Xiao, Xuefeng] Tianjin Univ Tradit Chinese Med, Tianjin 300193, Peoples R China.
通讯机构：
[Zhang, YQ; Yang, HJ] C;[Xiao, Xuefeng] T;China Acad Chinese Med Sci, Inst Chinese Mat Med, 16 Nanxiaojie, Beijing 100700, Peoples R China.;Tianjin Univ Tradit Chinese Med, Tianjin 300193, Peoples R China.
关键词：
Chinese patent drug;Coronary heart Disease;Network pharmacology;Pharmacological mechanism;Traditional Chinese medicine;Xueshuan-Xinmai-Ning tablet
摘要：
BACKGROUND: Xueshuan-Xinmai-Ning Tablet (XXNT), a commercially available patent drug, has been extensively used in the treatment of coronary heart disease (CHD) with a satisfying therapeutic efficacy. The aim of this study was to explore the underlying pharmacological mechanisms of XXNT acting on CHD. STUDY DESIGN: An integrative pharmacology-based investigation was performed. METHOD: Putative targets of composite compounds contained in XXNT were predicted using the Drug Target Prediction Tool in the Computation Platform for Integrative Pharmacology of Traditional Chinese Medicine (TCMIP, www.tcmip.cn) and MedChem Studio. Then, an interaction network of XXNT putative targets-known CHD-related genes was constructed, and candidate XXNT targets related to its therapeutic effects on CHD were identified by calculating three major network topological features. Functional enrichment analysis was performed to investigate the specific functions and pathways involved by the candidate XXNT targets acting on CHD, which were further validated by in vitro experiments. RESULTS: A total of 742 putative targets hit 126 chemical components contained in XXNT were predicted. Following the construction of XXNT putative target-known CHD-related gene network, and the network topological feature calculation, we identified 51 candidate XXNT targets related to its therapeutic effects on CHD. Functionally, these candidate XXNT targets were significantly associated with various cardiovascular system-related pathways, sedation-related pathways, inflammatory and immune-related pathways and endocrine/metabolic system-related pathways. More importantly, the in vitro experiment validation confirmed the regulatory effects of XXNT in SRC, VEGF and VEGFR-1, which play roles in VEGF signaling pathway, based on the endothelial injury cell model. CONCLUSION: Our findings reveal that XXNT may attenuate the major pathological changes of CHD through regulating its candidate targets, which might be involved into the signal transductions in nervous-endocrine-immune-cardiovascular-metabolic system.
作者机构：
[Luo, Yun; Zhou, Ping; Sun, Xiaobo; Qin, Meng; Sun, Guibo; Lu, Shan] Chinese Acad Med Sci, Inst Med Plant Dev, 151 Malianwa North Rd, Beijing 100193, Peoples R China.;[Luo, Yun; Zhou, Ping; Sun, Xiaobo; Qin, Meng; Sun, Guibo; Lu, Shan] Peking Union Med Coll, 151 Malianwa North Rd, Beijing 100193, Peoples R China.;[Luo, Yun; Zhou, Ping; Sun, Xiaobo; Qin, Meng; Sun, Guibo; Lu, Shan] Beijing Key Lab Innovat Drug Discovery Tradit Chi, Beijing, Peoples R China.;[Luo, Yun; Zhou, Ping; Sun, Xiaobo; Qin, Meng; Sun, Guibo; Lu, Shan] Minist Educ, Key Lab Bioact Subst & Resource Utilizat Chinese, Beijing, Peoples R China.;[Luo, Yun; Zhou, Ping; Sun, Xiaobo; Qin, Meng; Sun, Guibo; Lu, Shan] State Adm Tradit Chinese Med, Key Lab Efficacy Evaluat Chinese Med Glyeolipid M, Beijing, Peoples R China.
通讯机构：
[Sun, GB; Sun, XB] C;[Sun, GB; Sun, XB] P;Chinese Acad Med Sci, Inst Med Plant Dev, 151 Malianwa North Rd, Beijing 100193, Peoples R China.;Peking Union Med Coll, 151 Malianwa North Rd, Beijing 100193, Peoples R China.
摘要：
Editor: Timothy R. Elliott, PhDQuarterly, beginning in FebruaryRehabilitation Psychology is a quarterly peer-reviewed journal that publishes articles in furtherance of the mission of Division 22 (Rehabilitation Psychology) of the American Psychological Association and to advance the science and practice of rehabilitation psychology.Rehabilitation psychologists consider the entire network of biological, psychological, social, environmental, and political factors that affect the functioning of persons with disabilities or chronic illness. Given the breadth of rehabilitation psychology, the journal's scope is broadly defined. Suitable submissions include papers describing experimental investigations, survey research, evaluations of specific interventions, outcome studies, historical perspectives, relevant public policy issues, conceptual/theoretical formulations with implications for clinical practice, reviews of empirical research, detailed case studies, and professional issues.Papers will be evaluated for their importance to the field, scientific rigor, novelty, suitability for the journal, and clarity of writing. The primary determinant of editorial decisions is whether the paper enlarges both the understanding of important psychological problems in rehabilitation and the capacity to offer effective assistance in ameliorating those problems.Coverage in the Journals@Ovid database begins with the Spring 1995 issue.
作者机构：
[Li, Jiayu; Zhang, Shuihan; Liang, Xuejuan; Chen, Lin; Shen, Bingbing] Hunan Acad Chinese Med, Res Inst Chinese Med, Changsha, Hunan, Peoples R China.;[Zhou, Rongrong] Changchun Univ Chinese Med, Sch Pharm, Changchun, Jilin, Peoples R China.;[Li, Jiayu; Yang, Yupei] Hunan Univ Chinese Med, Sch Pharm, Changsha, Hunan, Peoples R China.;[Huang, Luqi] China Acad Chinese Med Sci, Natl Resource Ctr Chinese Mat Med, Beijing, Peoples R China.
通讯机构：
[Zhang, Shuihan] H;Hunan Acad Chinese Med, Res Inst Chinese Med, Changsha, Hunan, Peoples R China.
摘要：
This paper intends to identify the antimicrobial activity compounds from the deciduous leaves of Malus doumeri (Dong Li Tea) by HPLC-ESI-QTOF-MS/MS. The ethanol extracts of Malus doumeri were partitioned into petroleum ether, dichloromethane, ethyl acetate, n-butanol and water fraction, respectively. The antimicrobial screening experiments showed that ethyl acetate fraction has a certain antibacterial activity by inhibition zone method in vitro. And then we used the HPLC-ESI-QTOF-MS/MS method to verify the identities of bioactive compounds. Finally, 41 compounds were determined and 11 of which were firstly reported in this plant. Notably, compounds (32, 34, 38) are new dihydrochalcones, and three chlorogenic acid analogues (10, 13, 17) may be potential antimicrobial active ingredient. Which is of great significance to the isolation of novel compounds and the discovery of new natural preservative candidates from the deciduous leaves of Malus doumeri.
摘要：
Five new compounds, including one bisabolane-type sesquiterpenoids, namely aspergillusene D (1), two new xanthones (2 and 3), and two new catecholderivatives (4 and 5), together with fourteen known compounds (6-19), were isolated and identified from the fungus Aspergillus sydowiiSCSIO 41,301 from the sponge Phakellia fusca. Their structures and absolute configurations were determined by extensive spectroscopic analysis and electronic circular dichroism (ECD) calculations. Most of the isolated compounds (1-3, and 6-19) were evaluated for their antiviral, cytotoxic, and antibacterial activities. Among them, new compounds 2 and 3 displayed obvious selective inhibitory activities against two influenza A virus subtypes, including A/Puerto Rico/8/34 (H1N1) and A/FM-1/1/47 (H1N1), with IC50 values ranging from 2.17 +/- 1.39 to 4.70 +/- 1.11 mu M.
作者：
Khan, Asmat Ullah;Akram, Muhammad;Daniyal, Muhammad;Zainab, Rida*
期刊：
International Journal of Neuroscience,2019年129(1):55-93 ISSN：0020-7454
通讯作者：
Zainab, Rida
作者机构：
[Khan, Asmat Ullah] Univ Sao Paulo, Ribeirao Preto Med Sch, Lab Neuroanat & Neuropsychobiol, Dept Pharmacol,FMRP, Sao Paulo, Brazil.;[Khan, Asmat Ullah] Univ Poonch Rawalakot, Sch Med & Hlth Sci, Dept Eastern Med & Surg, Rawalakot, Pakistan.;[Zainab, Rida; Akram, Muhammad] Govt Coll Univ, Directorate Med Sci, Dept Eastern Med & Surg, Old Campus,Allama Iqbal Rd, Faisalabad 38000, Pakistan.;[Daniyal, Muhammad] Hunan Univ Chinese Med, Sch Pharm, TCM & Ethnomed Innovat & Dev Lab, Changsha, Hunan, Peoples R China.;[Daniyal, Muhammad] Hunan Univ, Coll Biol, State Key Lab, Hunan Prov Key Lab Plant Funct Genom & Dev Regula, Changsha, Hunan, Peoples R China.
通讯机构：
[Zainab, Rida] G;Govt Coll Univ, Directorate Med Sci, Dept Eastern Med & Surg, Old Campus,Allama Iqbal Rd, Faisalabad 38000, Pakistan.
关键词：
Parkinson's disease;dopaminergic pathways;motor system;neostriatum;substantia nigra pars compacta
摘要：
Context: Parkinson's disease is the second common progressive neurodegenerative disease, distressing older men and is prevalent Worldwide. Objectives: This paper is aimed to review the epidemiology, etiology, pathogenesis, clinical manifestation, diagnosis and management of Parkinson disease. Methods: A google search was performed to recognize studies that review the characteristics of Parkinson disease. Search terms included " Parkinson's disease", " epidemiology", " etiology", " pathogenesis", " clinical manifestations", " diagnosis" and " management of Parkinson disease". Results: Parkinson's disease is linked to factors such as environmental chemicals, aging, family history and pesticide exposure such as the use of synthetic heroin. Parkinson's disease is characterized clinically by tremors at rest, postural instability, expressionless countenance, lead pipe rigidity and less commonly cognitive impairment. After 60 years of age, Parkinson's disease is commonly prevalent in 1% to 2% of the population, no racial differences are apparent, but the prevalence of Parkinson's disease is more common in men than women. There has also been a better understanding that the disorder may be linked with major non-motor trouble in addition to the additional generally recognized motor complications. There are various management options for the timely management of Parkinson disease. As the ailment advances, further management strategies are existing; however, the management of non-motor manifestations and late stage motor complications remains mainly testing and will advantage from additional clinical studies. Conclusion: In this article we have discussed current progress in the understanding of the epidemiology, clinical manifestations, pathogenesis and management strategies of the disease.
摘要：
Except for an essential step for the pathology of multiple diseases including atherosclerosis and rheumatoid arthritis, inflammation is an imperative therapeutic target for developing novel approaches for pharmacological interventions. Thus, molecular understanding of inflammation not only revealed the mechanisms of drug action and their biological targets but also has spawned innovative maneuvers to influence multifaceted biological systems, providing new prospects for drug designing and suggesting important new implications for existing clinical medicine. Meanwhile, modulation of inflammation with the use of medicinal plants proposed an alternate to conventional therapeutic strategies for numerous ailments, particularly when suppression of inflammation is expected. In modern literature, several species of medicinal plants have been shown substantial antiinflammatory and immunomodulatory actions including inhibitory effects on suppression of cellular and humoral immunity, lymphocyte activation, and propagation of apoptosis. Herein, we reviewed the molecular pharmacology of inflammation, chemical components and biological activities of medicinal plants such as, curcumin from Curcuma longa, and epigallocatechin-3-gallate from Camellia sinensis as well as their mechanism of action during inflammation at molecular level. An extensive review of the literature and electronic databases was conducted, encompassing PubMed, GoogleScholar, ScienceDirect, medlineplus, www.clinicaltrial.gov, www.fda.gov, www.ema.europa.eu, www.drugbank.ca, TrialBulletin.com, www.theplantlist.org, and www.pharmacodia.com for assembling the information. Additionally, data was attained from books, ethnopharmacological literature, and relevant publications for essential elements of molecular mechanisms, signal transduction networks, transcription factors, complement system, reactive species, and clinical trials are selected for substantial understanding of biochemistry, pathophysiology as well as clinical importance of medicinal plants during inflammatory diseases.
作者机构：
[Liu, Bin; Dang, Wenya; Zhou, Hongyan; Tong, Chunyi] Hunan Univ, Coll Biol, Changsha 410082, Hunan, Peoples R China.;[Yang, Yupei; Wang, Wei; Liu, Yongbei] Hunan Univ Chinese Med, Sch Pharm, Innovat Mat Med Res Inst, TCM & Ethnomed Innovat & Dev Int Lab, Changsha 410208, Hunan, Peoples R China.
通讯机构：
[Liu, Bin; Wang, Wei] H;Hunan Univ, Coll Biol, Changsha 410082, Hunan, Peoples R China.;Hunan Univ Chinese Med, Sch Pharm, Innovat Mat Med Res Inst, TCM & Ethnomed Innovat & Dev Int Lab, Changsha 410208, Hunan, Peoples R China.
摘要：
As a highly conserved damage repair protein, Fpg can specifically recognize and digest 8-oxoG from a damaged DNA backbone. Meanwhile, DNAzyme, a single-stranded DNA with enzymatic activity, can cleave RNA in the presence of cofactors. In this study, we established a highly sensitive method for Fpg assay using a DNAzyme-mediated signal cascade amplification strategy. Based on the Fpg-dependent fluorescence response of the "turn-on" manner, we could reliably determine Fpg activity down to 0.14 U mL-1 with a linear response from 0.10 to 40 U mL-1 under optimal conditions. In addition, this strategy was successfully applied to analyze the kinetic parameter of Fpg with Km of 0.061 muM. Furthermore, the developed sensing system was used to screen the regulators of Fpg from natural compounds and antibiotics. These results indicated that all of the 14 natural compounds and 6 kinds of antibiotics deferentially showed an active effect on Fpg in vitro. In summary, these results show that the method not only provides an alternative for monitoring Fpg activity but also shows great potential for drug screening in the future.
作者机构：
[Chen, Yinfang] Hunan Univ Chinese Med, Coll Pharm, Changsha 410208, Hunan, Peoples R China.;[Yu, Riyue; Chen, Yinfang] Jiangxi Univ Tradit Chinese Med, Coll Pharm, Nanchang 330004, Jiangxi, Peoples R China.;[Yu, Riyue] Jiangxi Univ Tradit Chinese Med, Key Lab Pharmacol Tradit Chinese Med Jiangxi, Nanchang 330004, Jiangxi, Peoples R China.;[Xu, Guoliang; Li, Bingtao; Liu, Hongning; Liu, HN; Xu, GL; Jiang, Li; Zhang, Qiyun] Jiangxi Univ Tradit Chinese Med, Res Ctr Different & Dev TCM Basic Theory, Nanchang 330004, Jiangxi, Peoples R China.;[Xu, Guoliang; Li, Bingtao; Liu, Hongning; Liu, HN; Xu, GL; Jiang, Li; Zhang, Qiyun] Jiangxi Univ Tradit Chinese Med, Jiangxi Prov Key Lab TCM Etiopathogenisis, Nanchang 330004, Jiangxi, Peoples R China.
通讯机构：
[Liu, HN; Xu, GL] J;Jiangxi Univ Tradit Chinese Med, Res Ctr Different & Dev TCM Basic Theory, Nanchang 330004, Jiangxi, Peoples R China.;Jiangxi Univ Tradit Chinese Med, Jiangxi Prov Key Lab TCM Etiopathogenisis, Nanchang 330004, Jiangxi, Peoples R China.
关键词：
traditional Chinese medicine decoction;quality evaluation;UPLC-QTOF-MS;UFLC-QQQ-MS;ShenFu prescription decoction
摘要：
Decoction is one of the oldest forms of traditional Chinese medicine and it is widely used in clinical practice. However, the quality evaluation and control of traditional decoction is a challenge due to the characteristics of complicated constituents, water as solvent, and temporary preparation. ShenFu Prescription Decoction (SFPD) is a classical prescription for preventing and treating many types of cardiovascular disease. In this article, a comprehensive and rapid method for quality evaluation and control of SFPD was developed, via qualitative and quantitative analysis of the major components by integrating ultra-high-performance liquid chromatography equipped with quadrupole time-of-flight mass spectrometry and ultra-fast-performance liquid chromatography equipped with triple quadrupole mass spectrometry. Consequently, a total of 39 constituents were tentatively identified in qualitative analysis, of which 21 compounds were unambiguously confirmed by comparing with reference substances. We determined 13 important constituents within 7 min by multiple reaction monitoring. The validated method was applied for determining five different proportion SFPDs. It was found that different proportions generated great influence on the dissolution of constituents. This may be one of the mechanisms for which different proportions play different synergistic effects. Therefore, the developed method is a fast and useful approach for quality evaluation of SFPD.
摘要：
Parkinson's disease (PD) is a typical neurodegenerative disease and the pathological feature of which is the death of dopamine neurons in the substantia nigra region. At present, neuronal death caused by inflammatory cytokine-mediated neuroinflammation is being extensively studied. The nucleotide-binding oligomerization domain-, leucine-rich repeat and pyrin domain-containing 3 (NLRP3) inflammasome is an inflammatory complex existing in microglia. Its activation promotes the secretion of the inflammatory cytokine interleukin-1beta/18 (IL-1beta/18) and induces pyroptosis, a type of cell death that possesses the potential for inflammation, to rupture microglia to further release IL-1beta. In this review we focus on the mechanisms of activation of the NLRP3 inflammasome and pyroptosis and their inflammatory effects on the development of PD. In addition, we focus on some inhibitors of NLRP3 inflammatory pathways to alleviate the progression of PD by inhibiting central inflammation and provide new therapeutic strategies for the treatment of PD.
摘要：
The leaves of Cyclocarya paliurus with sweet taste are often used as herbal tea in People's Republic of China. In this study eight previously undescribed seco-dammarane type triterpenoids, cyclocariols A-H along with seven known compounds were isolated and characterized from its leaves. A possible biogenetic pathway for seco-dammarane type triterpenoids formation has been discussed. Cyclocariols A-H were evaluated for their cytotoxicities against human liver (SMMC-7721) and breast cancer (BT-549) cell lines. Cyclocariols A, B, E, and H were also tested against human colon tumor (HCT-116) cell lines, where all four exhibited good activities with IC50 values of 6.53, 4.94, 8.24, and 6.48 mu M, respectively. (C) 2017 Elsevier Ltd. All rights reserved.
期刊：
Cellular Physiology and Biochemistry,2018年45(5):1893-1903 ISSN：1015-8987
通讯作者：
Huang, Yongpan;Yi, Minhan
作者机构：
[Zhao, Shuang; Li, Chen; Zhang, Yanyan] Guizhou Med Univ, Key Lab Optimal Utilizat Nat Med Resources, Guiyang, Guizhou, Peoples R China.;[Zhang, Yuan] Cent S Univ, Xiangya Hosp, Dept Neurol, Changsha, Hunan, Peoples R China.;[Zhang, Yuan] Univ Michigan, Dept Neurol, Ann Arbor, MI 48109 USA.;[Tang, Jiayu] Brain Hosp Hunan Prov, Dept Neurol, Changsha, Hunan, Peoples R China.;[Huang, Yongpan] Hunan Acad Chinese Med, Inst Chinese Med, Dept Pharmacol, Changsha, Hunan, Peoples R China.
通讯机构：
[Huang, YP; Yi, MH] C;[Huang, YP; Yi, MH] H;Cent S Univ, Informat Secur & Big Data Res Inst, Changsha, Hunan, Peoples R China.;Hunan Acad Chinese Med Changsha, Changsha, Hunan, Peoples R China.
摘要：
Major depressive disorder (MDD) remains a major public health problem worldwide. The association between MDD and the dysfunction of gap junction channels (GJCs) in glial cells, especially astrocytes, is still controversial. This review provides an overview of the role of astrocyte GJCs in LMDD. Exposure to chronic unpredictable stress caused a reduction in connexin expression in the rat prefrontal cortex, a result that is consistent with clinical findings reported in postmortem studies of brains from MDD patients. Chronic antidepressant treatment in these rats increased the expression of connexins. However, pharmacological GJC blockade in normal rodents decreased connexin expression and caused depressive-like behaviors. Furthermore, GJC dysfunction affects electrical conductance, metabolic coupling and secondary messengers, and inflammatory responses, which are consistent with current hypotheses on MDD. All these results provide a comprehensive overview of the neurobiology of MDD. This review supports the hypothesis that the regulation of GJCs between astrocytes could be an underlying mechanism for the therapeutic effect of antidepressants.
作者机构：
[Liu, Hao-Ran; Kang, Lu; Men, Xue] Hunan Univ, Coll Chem & Chem Engn, Changsha 410082, Hunan, Peoples R China.;[Gao, Xiao-Hui] Changsha Med Univ, Coll Pharm, Changsha 410219, Hunan, Peoples R China.;[Wu, Hong-Nian; Yan, Jian-Ye; Cui, Pei-Wu] Hunan Univ Chinese Med, Coll Pharm, Changsha 410208, Hunan, Peoples R China.;[Oldfield, Eric] Univ Illinois, Dept Chem, 1209 W Calif St, Urbana, IL 61801 USA.
通讯机构：
[Liu, Hao-Ran; Yan, Jian-Ye] H;Hunan Univ, Coll Chem & Chem Engn, Changsha 410082, Hunan, Peoples R China.;Hunan Univ Chinese Med, Coll Pharm, Changsha 410208, Hunan, Peoples R China.
关键词：
Coumarin;Chalcone;Cholinesterase inhibitors;Structure-activity relationship;Tertiary amine group
摘要：
Chalcones containing tertiary amine side-chains have potent activity as acetylcholinesterase (AChE) inhibitors. However, the effects of the location of the tertiary amine groups as well as of other groups on AChE and butyrylcholinesterase (BChE) activity have not been reported. Here, we report the synthesis and testing of 36 new coumarin-chalcone hybrids (5d-7j, 9d-11f, 12k-13m) against AChE and BChE. The nature and position of the chalcone substituents had major effects on inhibitory activity as well as selectivity for AChE over BChE. Compounds with para-substituted chalcone fragments in which the substituents were choline-like had potent activity against AChE and poor activity against BChE, while ortho-substituted analogs exhibited an opposite effect. Replacement of the terminal amine groups by amide, alkyl or alkenyl groups abrogated activity. Compound 5e showed potent inhibitory activity (IC50 = 0.15 +/- 0.01 mu mol/L) and good selectivity for AChE over BChE (ratio 27.4), and a kinetic study showed that 5e exhibited mixed-type inhibition against AChE. Computational docking results indicate that 5e binds to Trp 279, Tyr334 and Trp 84 in AChE, but only to Trp 82 in BChE. Overall, the results show that coumarin-chalcone hybrids with choline-like side-chains have promising activity and selectivity against AChE and be promising therapeutic leads for Alzheimer's disease.
摘要：
Saponins such as notoginsenosides and ginsenosides from Panax notoginseng are responsible for the herb's clinical applications. Unfortunately, there is poor oral bioavailability of saponins. However, gut microbiota can transform saponins to yield the metabolites that are potential bioactive substances. In this study, we aimed to characterize the metabolic profiles of P. notoginseng saponins (PNS) by incubating them with human gut microbiota. The notoginsenosides, ginsenosides and related metabolites were separated and identified using a highly sensitive and selective high-performance liquid chromatography coupled with diode array detection/quadrupole tandem time-of-flight mass spectrometry (HPLC-DAD-Q-TOF-MS/MS). The results showed that the most abundant metabolites, ginsenoside F1, protopanaxatriol (PPT), ginsenoside Rh2, ginsenoside compound K (GCK) and protopanaxadiol (PPD), were reported to possess stronger related pharmacological activities when compared with parent ginsenosides. These metabolites were identified among a total of 45 other metabolites. Furthermore, it was elucidated that deglycosylation is the main metabolic pathway which saponins are split off from glycosyl moieties by the enzymes secreted from gut microbiota. The gut microbiota may play a significant role in mediating the bioactivities of PNS. (C) 2017 Elsevier B.V. All rights reserved.
