期刊：
JOURNAL OF AOAC INTERNATIONAL,2021年104(6):1652-1660 ISSN：1060-3271
通讯作者：
Hanwen Yuan<&wdkj&>Wei Wang
作者机构：
[Wang, Wei; Yang, Shuai; Qiu, Yixing; Liu, Yang; Yuan, Hanwen; Lyu, Mengying] Hunan University of Chinese Medicine, School of Pharmacy, Innovative Material Medical Research Institute, TCM and Ethnomedicine Innovation & Development International Laboratory, Changsha 410208, China
通讯机构：
[Hanwen Yuan; Wei Wang] H;Hunan University of Chinese Medicine, School of Pharmacy, Innovative Material Medical Research Institute, TCM and Ethnomedicine Innovation & Development International Laboratory , Changsha 410208, China
摘要：
BACKGROUND: The dried fruit of Chaenomeles speciosa, known as Chaenomelis Fructus or Zhoupi Mugua, is a type of traditional Chinese medicine (TCM) that is widely used to treat many diseases. Guangpi Mugua, the dried fruit of the Chaenomeles sinensis, is its most commonly known adulterant. OBJECTIVE: To establish a robust approach for the quality control and identification of Chaenomelis Fructus. METHOD: Thin-layer chromatography (TLC) was optimized and used to discriminate Chaenomelis Fructus from Guangpi Mugua. High-performance liquid chromatography (HPLC) combined with fingerprint analysis and partial least-squares (PLS) discrimination analysis (DA) was employed to study the chemical differences between Chaenomelis Fructus and Guangpi Mugua. The single standard to determine multi-components (SSDMC) method, with credible precision, repeatability, stability, and durability, was developed for quantitative analysis of the abundant markers. RESULTS: The developed TLC and HPLC methods were effective in the authentication of Chaenomelis Fructus. Moreover, oleanolic acid, ursolic acid, pomolic acid, corosolic acid, 3-O-acetylpomolic acid, and one unknown compound were identified to be critical markers for the discrimination of Chaenomelis Fructus from Guangpi Mugua. CONCLUSION: Adulteration has always been a challenge in the development of TCM. This study presents useful insights that may help solve the problem of adulteration during the preparation of Chaenomelis Fructus. HIGHLIGHTS: The present study provides a systematic method for the quality control of Chaenomelis Fructus. This is therefore the first step towards solving the problem of adulteration to improve the clinical safety and effectiveness of Chaenomelis Fructus.
摘要：
ETHNOPHARMACOLOGICAL RELEVANCE: Kadsura heteroclita (Roxb.) Craib (traditionally known as "Xue Tong") is an important member of the economically and medicinally important plant family Schisandraceae. "Xue Tong" is an imperative ingredient of the Tujia ethnomedicine, traditionally used for the treatment of rheumatoid arthritis (RA), hepatitis, and muscles and joint spasm. The plant is known to be a rich source of lignans and triterpenoids. These classes of natural products have been known to possess various pharmacological activities. AIM OF REVIEW: This review was motivated by the importance of K. heteroclita in traditional Chinese medicine (TCM). It aims to compile the available information on its botanical distribution and description, traditional uses, phytochemistry, pharmacological activities, toxicity, and quality control to provide a solid base for further research and development. MATERIALS AND METHODS: Relevant literature was collected by several scientific databases including PubMed, CNKI, Scifinder, The Plant List, Google Scholar, Baidu Scholar, Books (Tujia pharmaceutical records, Guangxi Chinese herbal medicine, Hunan pharmaceutical records and Field identification manual of Chinese herbal medicine) and other literature sources (Flora of China, Pharmacopoeia of the People's Republic of China) which helped in collecting maximum data about the studied species. RESULTS: Traditional uses of K. heteroclita have proven its medicinal importance, providing a rationale for scientific research. Phytochemical studies on the stem of K. heteroclita resulted in the identification of 187 chemical constituents, among which lignans and triterpenoids are the predominant groups. The isolates and crude extracts have been found to exhibit a wide spectrum of in vivo and in vitro pharmacological activities such as anti-RA, anti-inflammatory and analgesic, hepatoprotection, anti-HIV, anti-cancer and anti-HBV. Schisanlactone E (xuetongsu), a triterpenoid, is one of the major components of K. heteroclita exhibiting anti-cancer, neuroprotective and anti-neuroinflammation activities. Interestingly and luckily, this plant has been found to be safe and non-toxic within the therapeutic dose range. CONCLUSION: Pharmacological investigations have validated the use of K. heteroclita in traditional Chinese medicine (TCM). Literature review has demonstrated that lignans and triterpenoids are possibly responsible for most of the biological activities exhibited by this plant. To conclude, this plant shows immense potential for the discovery of more potent bioactive secondary metabolites and therefore further phytochemical and biological studies on other parts of K. heteroclita need to be conducted and more compounds need to be tested regarding their biological activities to completely explore its value as a tremendously important medicinal plant species.
期刊：
Chemical Communications,2020年56(21):3229-3232 ISSN：1359-7345
通讯作者：
Liu, Bin;Wang, Wei
作者机构：
[Liu, Bin; Fan, Jialong; Zhou, Ting; Tong, Chunyi; Hu, Yalei] Hunan Univ, Coll Biol, Hunan Prov Key Lab Plant Funct Genom & Dev Regula, Changsha 410082, Peoples R China.;[Qin, Yan; Wang, Wei; Xie, Qian] Hunan Univ Chinese Med, TCM & Ethnomed Innovat & Dev Int Lab, Innovat Mat Med Res Inst, Sch Pharm, Changsha 410208, Peoples R China.
通讯机构：
[Liu, Bin; Wang, Wei] H;Hunan Univ, Coll Biol, Hunan Prov Key Lab Plant Funct Genom & Dev Regula, Changsha 410082, Peoples R China.;Hunan Univ Chinese Med, TCM & Ethnomed Innovat & Dev Int Lab, Innovat Mat Med Res Inst, Sch Pharm, Changsha 410208, Peoples R China.
摘要：
We report an intracellular imaging and assay nanoplatform for RNase A using a DNA tetrahedron-based fluorescent probe as a substrate. Importantly, a natural compound was used as an RNase A activity stimulator to improve the sensitivity. This platform provides an alternative for the diagnosis and prognosis of RNase A-related diseases and drug screening.
作者机构：
[肖美凤] College of Pharmacy, Hunan University of Chinese Medicine Changsha 410208, China Hunan Provincial Key Laboratory of Drugability and Preparation Modification of Traditional Chinese Medicine Changsha 410208, China;[段晓鹏] College of Pharmacy, Hunan University of Chinese Medicine Changsha 410208, China Supramolecular Mechanism and Mathematic-Physics Chracterization for Chinese Materia Medica, Hunan University of Chinese Medicine Changsha 410208, China;[邓凯文] The First Affinity Hospital, Hunan University of Chinese Medicine Changsha 410007, China;[刘文龙; 贺福元; 杨岩涛] College of Pharmacy, Hunan University of Chinese Medicine Changsha 410208, China Hunan Provincial Key Laboratory of Drugability and Preparation Modification of Traditional Chinese Medicine Changsha 410208, China Supramolecular Mechanism and Mathematic-Physics Chracterization for Chinese Materia Medica, Hunan University of Chinese Medicine Changsha 410208, China
通讯机构：
The First Affinity Hospital, Hunan University of Chinese Medicine, Changsha, China
摘要：
Long noncoding RNA-steroid receptor RNA activator (LncRNA-SRA) is transcribed from a class of noncoding genes, and plays a critical role in regulating cell proliferation. However, the effect of lncRNA-SRA remains unclear in vascular proliferative diseases. In the present study, we overexpressed lncRNA-SRA in vitro, then investigated the biological consequences. A vascular damage mice model was constructed by performing femoral artery wire injury. LncRNA-SRA was overexpressed in the injured arteries, and significantly promoted the expression of ki67, thereby caused an overall increase in neointima formation. LncRNA-SRA overexpression led to the proliferation and migration of vascular smooth muscle cells (VSMCs). By stimulating the phosphorylation of MEK, ERK and CREB (cyclic nucleotide responsive element binding protein), lncRNA-SRA promoted VSMC proliferation. Meanwhile, these effects were blocked by the MEK inhibitor U0126. Therefore, lncRNA-SRA promoted VSMC proliferation by activating the MEK-ERK-CREB pathway. LncRNA-SRA could be a promising therapeutic target in vascular diseases characterized by neointimal hyperplasia.
作者机构：
[XunLi; Yang, Songwei; Peng, Ye; Chen, Naihong; Liu, Yingjiao; Wen, Birui; Ren, Siyu] Hunan Univ Chinese Med, Coll Pharm, Changsha 410208, Hunan, Peoples R China.;[Chen, Naihong; Chu, Shifeng] Chinese Acad Med Sci, Inst Mat Med, Beijing 100050, Peoples R China.;[Chen, Naihong; Chu, Shifeng] Peking Union Med Coll, Beijing 100050, Peoples R China.
通讯机构：
[Chen, Naihong] C;[Chen, Naihong] P;Chinese Acad Med Sci, Inst Mat Med, Beijing 100050, Peoples R China.;Peking Union Med Coll, Beijing 100050, Peoples R China.
摘要：
Panax notoginseng saponins (PNS) are the major components of Panax notoginseng, with multiple pharmacological activities but poor oral bioavailability. PNS could be metabolized by gut microbiota in vitro, while the exact role of gut microbiota of PNS metabolism in vivo remains poorly understood. In this study, pseudo germ-free rat models were constructed by using broad-spectrum antibiotics to validate the gut microbiota-mediated transformation of PNS in vivo. Moreover, a high performance liquid chromatography-electrospray ionization tandem mass spectrometry (HPLC-ESI-MS/MS) was developed for quantitative analysis of four metabolites of PNS, including ginsenoside F1 (GF1), ginsenoside Rh2 (GRh2), ginsenoside compound K (GCK) and protopanaxatriol (PPT). The results showed that the four metabolites could be detected in the control rat plasma, while they could not be determined in pseudo germ-free rat plasma. The results implied that PNS could not be biotransformed effectively when gut microbiota was disrupted. In conclusion, gut microbiota plays an important role in biotransformation of PNS into metabolites in vivo.
摘要：
Five undescribed diterpenoid alkaloids tanguticulines A-E and two undescribed amide compounds 5-methoxy-N-Salicylanthranilic acid methyl ester and 3, 5-dimethoxy-4- hydroxycinnamamide-4-O-beta-D-glucopyranoside, were isolated from the whole plant of Aconitum tanguticum (Maxim.) Stapf. Their structures were assigned by detailed analysis of MS and NMR spectroscopic data. Compounds 1 and 5 were evaluated for their antivirus activities against influenza a (H1N1) virus in vitro. Both of them showed obvious inhibitory effect on the cytopathic changes induced by H1N1, with IC50 values of 2.9mugmL(-1) and 2.4mugmL(-1), respectively.
摘要：
As part of our continual efforts to exploit Tujia Ethnomedicine' for their pharmacophoric functionalities, we herein investigated Kadsura heteroclita collected from a deep Wulin mountain area in northern Hunan province. The current study resulted in the isolation of three new sesquiterpenes: 6,9,15-trihydroxycadinan-4-en-3-one (1), (+)-3,11,12-trihydroxycalamenene (2), (-)-3,10,11,12-tetrahydroxy-calamenene (3), along with four known sesquiterpenes (4-7), and a cytochalasin H (8). Their chemical structures were elucidated by 1D-, and 2D-NMR spectroscopy, and HRESI-MS, CD spectrometry. The antioxidant, and cytotoxic activities of the compounds were evaluated. Compound 8 exhibited a strong antioxidant effect with an IC50 value of 3.67 mu M on isolated human polymorphonuclear cells or neutrophils.
作者机构：
[文雯] Department of Pharmaceutics,Hunan University of Chinese Medicine Changsha 410208,China First Affiliated Hospital of Hunan University of Chinese Medicine Changsha 410007,China;[刘文龙; 贺福元; 周逸群] Department of Pharmaceutics,Hunan University of Chinese Medicine Changsha 410208,China Hunan Provincial Key Laboratory of Druggability and Preparation Modification of Traditional Chinese Medrcine Changsha 410208,China;[刘红宇] First Affiliated Hospital of Hunan University of Chinese Medicine Changsha 410007,China
通讯机构：
First Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, China
作者机构：
[Pan, Wei-Dong; Zhang, Ni; Yi, Ping; Wan, Ke; Jiang, Sai; Zhou, Mei; Lou, Hua-Yong] Guizhou Med Univ, State Key Lab Funct & Applicat Med Plants, Guiyang 550014, Guizhou, Peoples R China.;[Song, Zhi-Qin; Wu, Ming-Kai] Guizhou Acad Agr Sci, Inst Modern Chinese Herbal, Guiyang 550006, Guizhou, Peoples R China.;[Wang, Wei; Jiang, Sai] Hunan Univ Chinese Med, Innovat Mat Med Res Inst, Sch Pharm, TCM & Ethnomed Innovat & Dev Int Lab, Changsha 410208, Hunan, Peoples R China.
通讯机构：
[Pan, Wei-Dong; Wu, Ming-Kai] G;Guizhou Med Univ, State Key Lab Funct & Applicat Med Plants, Guiyang 550014, Guizhou, Peoples R China.;Guizhou Acad Agr Sci, Inst Modern Chinese Herbal, Guiyang 550006, Guizhou, Peoples R China.
摘要：
Ten previously undescribed bibenzyl derivatives (bletistrins A-J), including 5 that have hydroxyl-substituted chiral centres on the aliphatic bibenzyl bridge, along with twelve known bibenzyl derivatives, were isolated from the rhizomes of Bletilla striata. The structures of bletistrins A-J were primarily elucidated on the basis of their 1D and 2D NMR spectroscopic data. The absolute configurations of bletistrins A, D, F, H and I were determined by electronic circular dichroism (ECD) spectroscopic analysis and optical rotation value. Most of the isolated compounds were evaluated for their antibacterial activities against 3 g-positive bacterial strains and 1 g-negative bacterial strain. Bletistrins F, G, and J, bulbocol, shanciguol and shancigusin B showed inhibitory activities, with MICs of (3-28 mu g/mL) against S. aureus ATCC 6538.
摘要：
Type 2 diabetes mellitus (T2DM) is a chronic degenerative endocrine and metabolic disease with high mortality and morbidity, yet lacks effective therapeutics. We recently generated a novel fusion peptide INSR-IgG4Fc, Yiminsu (YMS), to facilitate the high-affinity binding and transportation of insulin. Thus, the aim of the present study was to determine whether the novel recombinant peptide, YMS, could contribute to restoring insulin sensitivity and glycaemic control in insulin resistance models and revealing its underlying mechanism. Palmitic acid (PA)-treated LO2 cells and high fat diet (HFD)-fed mice were treated with YMS. Therapeutic effects of YMS were measured using Western blotting, ELISA, qPCR, Histology and transmission electron microscopy. We observed that YMS treatment effectively improved insulin signaling in PA-treated LO2 cells and HFD-fed mice. Notably, YMS could significantly reduce serum levels of glucose, triglycerides, fatty acids and cholesterol without affecting the serum insulin levels. Moreover, our data demonstrated that YMS could restore glucose and lipid homeostasis via facilitating insulin transportation and reactivating PI3K/Akt signaling in both PA-treated cells and liver, gastrocnemius and brown fat of HFD-fed mice. Additionally, we noticed that the therapeutic effects of YMS was similar as rosiglitazone, a well-recognized insulin sensitizer. Our findings suggested that YMS is a potentially candidate for pharmacotherapy for metabolic disorders associated with insulin resistance, particularly in T2DM.
期刊：
Research Reviews in Pharmacology,2019年103(5-6):282-290 ISSN：0031-7012
通讯作者：
Qin, Li;Huang, Hong-Lin
作者机构：
[Shi, Ya-Ning; Zhang, Chan-Juan; Liao, Duan-Fang; Qin, Li] Hunan Univ Chinese Med, Sch Pharm, 300 Xueshi Rd, Changsha 410208, Hunan, Peoples R China.;[Shi, Ya-Ning; Zhang, Chan-Juan; Liao, Duan-Fang; Qin, Li] Hunan Univ Chinese Med, Div Stem Cell Regulat & Applicat, Changsha, Hunan, Peoples R China.;[Huang, Hong-Lin; Zhang, Xiu-Qin] Univ South China, Dept Pharmacol, 28 Changsheng West Rd, Hengyang 421001, Hunan, Peoples R China.;[Hu, Zhe-Yu] Cent S Univ, Hunan Canc Hosp, Changsha, Hunan, Peoples R China.;[Hu, Zhe-Yu] Cent S Univ, Affiliated Canc Hosp, Xiangya Med Sch, Changsha, Hunan, Peoples R China.
通讯机构：
[Qin, Li] H;[Huang, Hong-Lin] U;Hunan Univ Chinese Med, Sch Pharm, 300 Xueshi Rd, Changsha 410208, Hunan, Peoples R China.;Univ South China, Dept Pharmacol, 28 Changsheng West Rd, Hengyang 421001, Hunan, Peoples R China.
关键词：
Apoptosis;Cardiomyocytes;Genistein;Hypoxia
摘要：
BACKGROUND/AIMS: Hypoxia can induce cell injury in cardiomyocytes and further lead to cardiovascular diseases. Genistein (Gen), the predominant isoflavone found in soy products, has shown protective effects on cardiovascular system. The aim of the present study was to investigate the cardioprotective effect of Gen against chemical hypoxia-induced injury. METHODS: Cobalt chloride (CoCl2) was administrated to trigger chemical hypoxia in H9c2 cardiomyocytes. Cell proliferation was detected by using MTT assay. The expression level of hypoxia-related proteins (hypoxia-inducible factor [HIF]-1alpha and Notch-1) and apoptosis-related proteins (B cell lymphoma [Bcl]-2, Bax, and caspase-3) were evaluated by Western blot analysis. RESULTS: In response to hypoxia, cell viability was reduced dramatically, whereas the expression of HIF-1alpha was upregulated. Hypoxia also induced cardiomyocytes apoptosis by reducing the ratio of Bcl-2/Bax and increasing expression of caspase-3. Interestingly, Gen attenuated CoCl2-induced cell death and suppressed HIF-1alpha expression, as well as upregulated the expression of Notch-1. Furthermore, Gen could antagonize CoCl2-induced apoptosis through upregulating Bcl-2/Bax ratio and inhibiting caspase-3 expression. CONCLUSIONS: Gen prevents chemical hypoxia-induced cell apoptosis through inhibition of the mitochondrial apoptotic pathway, exerting protective effects on H9c2 cardiomyocytes.
作者机构：
[Wang, Yi; Lv, Jingwei; Lu, Cong; Jiang, Ning; Xu, Pan; Dong, Liming; Liu, Xinmin] Chinese Acad Med Sci, Res Ctr Pharmacol & Toxicol, Inst Med Plant Dev IMPLAD, Beijing, Peoples R China.;[Wang, Yi; Lv, Jingwei; Lu, Cong; Jiang, Ning; Xu, Pan; Dong, Liming; Liu, Xinmin] Peking Union Med Coll, Beijing, Peoples R China.;[Zhang, Hongxia] Hunan Univ Chinese Med, Div Stem Cell Regulat & Applicat, Changsha, Hunan, Peoples R China.;[Liu, Xinmin] Chinese Acad Med Sci, Inst Med Plant Dev, Beijing, Peoples R China.
通讯机构：
[Liu, Xinmin] P;[Liu, Xinmin] C;Peking Union Med Coll, Beijing, Peoples R China.;Chinese Acad Med Sci, Inst Med Plant Dev, Beijing, Peoples R China.
摘要：
Objectives: Chronic stress exposure can disrupt the balance of organisms, result in learning and memory impairments and induce oxidative stress. However, there is a lack of safe and effective long-term therapeutic agents for stress-related injuries. Fresh ginseng (FG), an unprocessed raw root of ginseng, has antioxidant and neuroprotective activities and has been used as functional health food in Asian countries for many years. The aim of this study was to verify the protective effects of FG on chronic restraint stress (CRS)-induced learning and memory impairments as well as oxidative stress damage in mice. Methods: Animals were subjected to object location recognition test (OLRT) and novel object recognition test (NORT) to evaluate discriminative ability and spatial learning and memory, and Morris water maze test (MWMT) was used to evaluate the acquisition and retention of spatial memory. In addition, oxidative stress parameters were assessed by measuring the malondialdehyde (MDA) and total antioxidant reactivity levels in serum. Results: Experimental results demonstrated that CRS-induced mice exhibited significantly decreased discrimination index (DI) in OLRT and NORT, longer escape latency and swimming distance, and decreased crossing numbers in MWMT. FG (2 and 6 g/kg) treatment markedly enhanced the discriminative ability by elevating DI in OLRT and NORT, improved the acquisition and retention of spatial memory by decreasing escape latency and swimming distance in the acquisition phase, and increased the crossing numbers in the probe phase of MWMT. Administration of FG (2 and 6 g/kg) significantly reduced the elevated MDA level caused by CRS. Discussion: Our results suggest that FG treatment could improve CRS-induced learning and memory impairments and oxidative stress damage. FG is an intriguing therapeutic agent and functional health food in stress-related dementia.
作者机构：
[林丽美; 刘月新; Lin, Yan; 吴萍; 肖榕; 廖端芳] Key Laboratory for Quality Evaluation of Bulk Herbs, College of Pharmacy, Hunan University of Chinese Medicine Changsha 410208, China;[林艳] College of Physics and Electronic, Central South University Changsha 410012, China;[李春] Key Laboratory for Quality Evaluation of Bulk Herbs, College of Pharmacy, Hunan University of Chinese Medicine Changsha 410208, China Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China
通讯机构：
Key Laboratory for Quality Evaluation of Bulk Herbs, College of Pharmacy, Hunan University of Chinese Medicine, Changsha, China
作者机构：
[Wang, Junfeng; Zhao, Biqing; Zhou, Xiaojiang; Qin, Li] Hunan Univ Chinese Med, Coll Pharm, Changsha 410208, Hunan, Peoples R China.;[Wang, Junfeng; Liu, Yonghong] Chinese Acad Sci, CAS Key Lab Trop Marine Bioresources & Ecol, Guangdong Key Lab Marine Mat Med, RNAM Ctr Marine Microbiol,South China Sea Inst Oc, Guangzhou 510301, Guangdong, Peoples R China.;[Cai, Liang] Hunan Prov Ctr Dis Control & Prevent, Changsha 410005, Hunan, Peoples R China.;[Zhong, Zhenping] Yao Sheng Tang Hu Nan Pharmaceut Co Ltd, Anxiang 415600, Peoples R China.
通讯机构：
[Zhou, Xiaojiang] H;Hunan Univ Chinese Med, Coll Pharm, Changsha 410208, Hunan, Peoples R China.
摘要：
Crotonols A and B (1 and 2), two tigliane diterpenoids featuring a rare C-7/C-14 cyclized and novel 5/7/7-fused carbon skeleton, along with the known tigliane wallichiioid A, were isolated from the leaves of Croton tiglium. Their structures were determined through spectroscopic methods, X-ray crystallography and ECD analysis. To the best of our knowledge, crotonol B (2) represents the first example of 13,14-seco-tigliane diterpenoids. Crotonols A and B displayed strong cytotoxic activities against the K562 cell line with IC50 values of 0.20 and 0.21 μM, respectively. Furthermore, crotonol A promoted the apoptosis of K562 cells through the cleavage of PARP and the accumulation of bax as well as the degradation of bcl-2.
摘要：
(1) Background: Chemokine-like factor 1 (CKLF1) is a chemokine with potential to be a target for stroke therapy. Compound IMM-H004 is a novel coumarin derivative screened from a CKLF1/C-C chemokine receptor type 4 (CCR4) system and has been reported to improve cerebral ischemia/reperfusion injury. This study aims to investigate the protective effects of IMM-H004 on cerebral ischemia injury and its infectious cardiopulmonary complications in adult and aged rats from the CKLF1 perspective. (2) Methods: The effects of IMM-H004 on the protection was determined by 2,3,5-triphenyltetrazolium chloride (TTC) staining, behavior tests, magnetic resonance imaging (MRI) scans, enzyme-linked immunosorbent assay (ELISA), Nissl staining, histo-pathological examination, and cardiopulmonary function detection. Immunohistological staining, immunofluorescence staining, quantitative real-time PCR (qPCR), and western blotting were used to elucidate the underlying mechanisms. (3) Results: IMM-H004 protects against cerebral ischemia induced brain injury and its cardiopulmonary complications, inhibiting injury, and inflammation through CKLF1-dependent anti-inflammation pathway in adult and aged rats. IMM-H004 downregulates the amount of CKLF1, suppressing the followed inflammatory response, and further protects the damaged organs from ischemic injury. (4) Conclusions: The present study suggested that the protective mechanism of IMM-H004 is dependent on CKLF1, which will lead to excessive inflammatory response in cerebral ischemia. IMM-H004 could also be a therapeutic agent in therapy for ischemic stroke and cardiopulmonary complications in the aged population.
