作者机构：
[资冬; 张君宇; 王婧; 任永镇; 朱伟] Technology Innovation Center, Hunan University of Chinese Medicine, Changsha, 410208, China;Hunan Key Lab of Powder and Innovative Drugs, Slate Key Lab of Ministry Training Bases, Changsha, 410208, China;[葛金文; 刘洋] Key Lab of Hunan Province for Prevention and Treatment of Integrated Traditional Chinese and Western Medicine on Cardiocerebral Diseases, Hunan University of Chinese Medicine, Changsha, 410208, China;[雷昌; 张秀丽] Technology Innovation Center, Hunan University of Chinese Medicine, Changsha, 410208, China, Hunan Key Lab of Powder and Innovative Drugs, Slate Key Lab of Ministry Training Bases, Changsha, 410208, China
作者机构：
[唐群; 朱伟; 刘春燕] Dept of Pathology, Hunan University of Chinese Medicine, Changsha, 410208, China;[杨胜辉] Dept of Preventive Medicine, Hunan University of Chinese Medicine, Changsha, 410208, China;[Zeng H.] Dept of Physiology, Hunan University of Chinese Medicine, Changsha, 410208, China
作者机构：
[朱伟; 唐群; 刘春燕] Dept of Pathology, Hunan University of Chinese Medicine, Changsha, 410208, China;[杨胜辉] Dept of Preventive Medicine, Hunan University of Chinese Medicine, Changsha, 410208, China;[Zeng H.] Dept of Physiology, Hunan University of Chinese Medicine, Changsha, 410208, China;[魏科] Dept of Microbiology, Hunan University of Chinese Medicine, Changsha, 410208, China
作者机构：
[Qiong LIU; Wei ZHU; Mailan LIU; Xia LIU; Jianan CAO; Shuning HU; Xiaorong CHANG] College of Acupuncture & Moxibustion & Tui-na, Hunan University of Chinese Medicine, Changsha 410007, China (湖南中医药大学针灸推拿学院, 长沙 410007, 中国);[Xianghong JING] Laboratory of Meridians and Collaterals, Institute of Acupuncture & Moxibustion, China Academy of Chinese Medical Sciences, Beijing 100700, China (中国中医科学院针灸研究所经络研究室, 北京 100700, 中国)
通讯机构：
[Xiaorong CHANG] C;[Xianghong JING] L;Laboratory of Meridians and Collaterals, Institute of Acupuncture & Moxibustion, China Academy of Chinese Medical Sciences, Beijing 100700, China (中国中医科学院针灸研究所经络研究室, 北京 100700, 中国)<&wdkj&>College of Acupuncture & Moxibustion & Tui-na, Hunan University of Chinese Medicine, Changsha 410007, China (湖南中医药大学针灸推拿学院, 长沙 410007, 中国)
摘要：
Objective: To observe the influence of acupuncture stimulus and thermal stimulus on the expression of mast cells(MCs) and 5-hydroxytryptamine(5-HT) in local acupoint area of Guanyuan (关元 CV 4) and serum 5-HT in rats. Methods: Thirty-nine male C57BL/6 rats were randomly divided into blank control group, acupuncture stimulus group and thermal stimulus group, with 13 rats in each group. The rats were stimulated by manual acupuncture at CV 4 for 5 min in acupuncture stimulus group, while those in thermal stimulus group were stimulated by adopting a thermal moxibustion apparatus on CV 4 for 30 min. The expression of MC and 5-HT in the skin in the acupoint area of CV 4 before and after acupuncture stimulus and thermal stimulus was observed and analyzed via adopting toluidine blue staining method and immunofluorescence histochemical method(5 rats were selected from each group), and the 5-HT content in serum before and after stimulus was determined through enzyme-linked immunosorbent assay(ELISA)(8 rats were selected from each group). The influence of acupuncture stimulus and thermal stimulus on the contents of MC and 5-HT in the skin and serum 5-HT in rats was analyzed and compared. Results:① After acupuncture stimulus or thermal stimulus on CV 4 of C57BL/6 rats, the number of MC in the acupoint area significantly increased when compared with that in blank group(the acupuncture stimulus group 12.40 ±2.07 vs. the blank group 3.00 ± 5.96; thermal stimulus group 26.20 ± 10.85 vs. the blank group 12.40± 2.07, both P< 0.05), and MC aggregation and degranulation were observed (the acupuncture stimulus group 17.80 ± 4.55 vs. the blank group 8.00 ± 3.16; the thermal stimulus group 24.00 ± 9.05 vs. the blank group 8.00 ± 3.16, P<0.05, P<0.01).② After acupuncture stimulus or thermal stimulus, 5-HT was released by MCs in the acupoint area, which aggregated around the blood vessels, and the number of 5-HT in the acupoint area significantly increased when compared with that in blank group (the blank group 3.00 ±1.28 vs. the acupuncture stimulus group 10.02 ± 3.21; the blank group 3.00 ± 1.28 vs. the thermal stimulus group 14.00 ± 3.94, both P < 0.01 ).③ Compared with blank group, both acupuncture stimulus and thermal stimulus could reduce the 5-HT content in serum(the blank group 0.72 ± 0.2372 vs. acupuncture stimulus group 0.43 ±0.21; the blank group 0.72 ± 0.24 vs. thermal stimulus group 0.32 ± 0.18, both P< 0.01), and the effect in thermal stimulus group was slightly superior to that in acupuncture stimulus group(P<0.05). Conclusion: Both acupuncture stimulus and thermal stimulus can cause the aggregation and degranulation of MCs and high expression of 5-HT in the acupoint area. The effect of thermal stimulus was superior to that of acupuncture stimulus in degranulation.
摘要：
急性肺损伤(ALI)是一种常见的临床疾病, 在全世界范围内引起严重的健康问题, 但其发病机理尚不清楚。自噬(autophagy)是当前生物医学研究热点之一, 涉及了多种病理生理过程。近期研究表明, 自噬与包含ALI在内的多种肺部疾病的发病进展有关。外泌体(exsome)是由多种活细胞分泌的, 由脂质双层被膜包围的纳米级结构(30~100 nm)。它通过传递其囊泡内包含的蛋白质, 脂质和核酸来调节细胞间通讯和相互作用。外泌体的存在对其供体细胞、受体细胞、以及细胞间结构都有着重要的生理和病理意义。因此, 外泌体的功能及特点得到越来越多的关注, 成为疾病的诊断和治疗策略发展的新目标。在本篇综述中, 我们概括了近年来自噬和外泌体对ALI病理生理的影响, 并提出了二者可能的关联假设。 Acute lung injury (ALI)is a common clinical disease that causes serious health problems worldwide, but the pathogenesis is still not clear. Autophagy is one of the current hot spots in biomedical research and involves a variety of pathophysiological processes. Recent studies have shown that autophagy is associated with the development of multiple lung diseases including ALI. Exosomes are nanoscale structures (30~100 nm)secreted by a variety of living cells and surrounded by lipid bilayer membranes. It regulates cell-cell communication and interactions by delivering proteins, lipids, and nucleic acids contained within its vesicles. The presence of exosomes has important physiological and pathological implications for their donor cells, recipient cells, and intercellular structures. Therefore, exploring the functions and characteristics of exosomes have received more and more attentions and become approaches for the diagnosis and treatment of diseases, new targets for strategic development. In this review, we summarize the pathophysiological effects of autophagy and exosomes in ALI in recent years and suggest possible associations between the two.
摘要：
Objective： To investigate the effect of Danlong Xingnao Decoction （DLXND, 丹龙醒脑方） on angiogenesis and bFGF protein expression in rats with cerebral ischemia-reperfusion. Methods： The MCAO rat model was created by modified Longa method, and blood reperfusion opened after 2 hours. The model rats were randomly divided into six groups, High-dose （14.76 g/kg）, moderate-dose （7.38 g/kg） and low-dose （3.69 g/kg） DLXND groups, nimodipine group （10.80 mg/kg）, model group and Sham group. The rats were given by garage once for 7 days. The microvessel vascular density （MVD） was observed and the expression of bFGF at protein level were studied in immuneohistochemical method. Results： Compared with the Sham group, the microvessel density and bFGF were higher significantly in model group （P〈0.05 or P〈0.01）. Compared with the model group, the levels of MVD and the protein expression of bFGF were significantly enhanced in DLXND groups （P〈0.05）. Conclusions： DLXND could promote angiogenesis and protect ischemic brain tissue in rats with cerebral ischemi- a-reperfusion, which mechanismsand may be related to upregulation of the bFGF expression.
