摘要：
Ethnopharmacological relevance: Buyang Huanwu Decoction (BYHWD), a Chinese prescription that has been used for hundreds of years to treat paralysis, has gained attention recently due to its significant neuroprotective properties. Aim of the study: This study was to investigate whether BYHWD treatment protected axotomized rubrospinal neurons (RN) following spinal cord injury (SCI) in rats. Materials and methods: Adult rats received a right lateral funiculus transection at the level between C3 and C4, and were either treated with BYHWD or with distilled water (DW) via gastrogavage. Effects on RN viability and atrophy were determined by Nissl staining, axon regeneration was examined by biotinylated dextran amine (BDA) tracing techniques and functional recovery was studied by a test of forelimb usage during spontaneous vertical exploration. Results: RN cell number and mean somal size were 20% and 29% higher, respectively, in BYHWD-treated rats relative to DW-treated rats. There were a small number of BDA-labeled axons in the caudal of injury site in BYHWD-treated rats, whereas no caudal axonal regeneration was detected in DW-treated rats. BYHWD-treated rats used the injured forelimb more often than rats treated with DW. Conclusions: These results indicate that administration of BYHWD following SCI protects injured neurons, promotes regeneration, and enhances functional recovery. (C) 2008 Elsevier Ireland Ltd. All rights reserved.
作者：
AN CHEN（陈安）;Hui WANG;Xiao-Qion WU;Yong-mei SHI（石咏梅）;Xue-Gang LUO;...
作者机构：
Department of Anatomy, Traditional Chinese Medicine University of Hunan, Changsha 410007, China;Department of Neurobiology, Central South University, Changsha 410013, China;Institute of Neurosciences, The Fourth Military Medical University, Xi'an 710032, China
会议名称：
中国神经科学学会第四次会员代表大会暨第七届全国学术会议(The 7th Biennial Meeting and the 5th Congress of the Chinese Society for Neuroscience)
会议时间：
2007-10-24
会议地点：
杭州
会议主办单位：
中国神经科学学会
会议论文集名称：
中国神经科学学会第四次会员代表大会暨第七届全国学术会议(The 7th Biennial Meeting and the 5th Congress of the Chinese Society for Neuroscience)论文集
摘要：
Objective To observe the effect ofBuyang Huanwu Decoction (BYHWD) on neuron protection.Methods SD rats were given BYHWD or distilled water via gastrogavage after right rubrospinal tract(RST) transection at the level between C3 and C4,8 week after treatment,rubrospinal neurons(RNs) were counted and mean cell size was examined by Nissl staining; concentration of cAMP in red nucleus was determined by radioimmunoassay; changes of BDNF and TrkB expression in RN was examed using immunohistochemistry.Results The number and mean cell size of RN was decreased by 22% and 35% respectively in the BYHWD group,42% and 64% respectively in the distilled water group; the expressions of cAMP,BDNF and TrkB were significantly increased in BYHWD group compared with distilled water group.Conclusion The present results suggest that administration of BYHWD following RST transection leads to promotion axotomized neuron survival and prevention their atrophy,the protective effects on axotomized neuron may be related to its modulation of cAMP and the increase of BDNF and TrkB on RN induced by cAMP.
作者：
An Chen（陈安）;Jian-Wei Zhang;Hui Wang;Liang Li（李亮）;Xue-Gang Luo;...
作者机构：
Department of Anatomy and Neurobiology, Central South University;Department of Anatomy, Traditional Chinese Medicine University of Hunan, Changsha;Institute of Neurosciences, The Fourth Military Medical University, Xi'an, China
会议名称：
4th International Neuroscience Symposium in China(第四届海内外华人神经科学研讨会)
会议时间：
2006-7-3
会议地点：
昆明·丽江
会议主办单位：
中国神经科学学会 中国生理学会 中国生物物理学会 中科院心理所行为遗传中心
会议论文集名称：
4th International Neuroscience Symposium in China(第四届海内外华人神经科学研讨会)论文集
