摘要：
AIM: To investigate the effect of Simotang (Decoction of Four Powered Drugs) on gastrointestinal motility, motilin and cholecystokinin expression in chronically stressed mice. METHODS: Forty mice were randomly divided into control group, stress group (model group), mosapride group and Simotang group, 10 in each group. A variety of unpredictable stimulations were used to induce chronic stress in mice. Then, the mice were treated with distilled water, mosapride or Simotang for 7 d. Gastric emptying and intestinal propulsion function were detected. Serum level of motilin was measured by enzyme-linked immunosorbent assay. Expression of cholecystokinin (CCK) in intestine, spinal cord and brain of mice was detected by immunohistochemistry and semi-quantitative reverse transcription polymerase chain reaction, respectively. RESULTS: Simotang improved the gastric emptying and intestinal propulsion in chronically stressed mice. Furthermore, the serum motilin level was significantly higher and the expression levels of CCK-positive cells and genes were significantly lower in intestine, spinal cord and brain of Simotang group than in those of model group (P < 0.05). No significant difference was found in serum motilin level and expression levels of CCK-positive cells and genes between the mosapride and Simotang groups. CONCLUSION: Simotang enhances the gastrointestinal motility in chronically stressed mice by regulating the serum motilin level and the expression of cholecystokinin
作者机构：
[Wu, Lu] Hunan Univ Tradit Chinese Med, Affiliated Tradit & Western Med Hosp 2, Changsha, Hunan, Peoples R China.;[Deng, Chang-Qing] Hunan Univ Tradit Chinese Med, Sch Integrated Chinese & Western Med, Pathophysiol Lab, Changsha, Hunan, Peoples R China.;[Tang, Ying-Hong] Hunan Univ Tradit Chinese Med, Dept Pharmacol, Changsha, Hunan, Peoples R China.;[Li, Hua; Zhang, Guo-Min; Chen, Bei-Yang] Hunan Univ Tradit Chinese Med, Dept Pathol, Changsha, Hunan, Peoples R China.;[Deng, Chang-Qing] Hunan Univ Tradit Chinese Med, Sch Integrated Chinese & Western Med, Pathophysiol Lab, Xiangzui Rd, Changsha, Hunan, Peoples R China.
通讯机构：
[Deng, Chang-Qing] H;Hunan Univ Tradit Chinese Med, Sch Integrated Chinese & Western Med, Pathophysiol Lab, Xiangzui Rd, Changsha, Hunan, Peoples R China.
关键词：
Total saponins of "panax notoginseng root";Atorvastatin;Vascular smooth muscle cell;Proliferating cell nuclear antigen;Cyclind D-1;Cycline;Extracellular matrix;Collagen I;Fibronectin;Matrix metalloproteinase-9;Tissue inhibitor metalloproteinase-1
摘要：
Aim of the study: the effect of total saponins of "panax notoginseng root" on aortic intimal hyperplasia and the expressions of cell cycle protein and extracellular matrix in rats Materials and methods: Sprague-Dawley rats were randomly divided into sham-operated, control, TSPN and atorvastatin group. Rat aorta intima in all groups were injured by insertion of domestic balloon catheter into the aortae except sham-operated rats. Drugs were administrated orally from the second day after vascular injury and continued for 14 days. The injured segments of aortae were collected on the sixteenth day after operation to observe the morphological changes of vascular structure and to examine the expressions of proliferating cell nuclear antigen(PCNA), cyclinD1, cyclinE, collagen I(Col-I), fibronect(FN), matrix metalloproteinase-9(MMP-9) and tissue inhibitor metalloproteinase-1(TIMP-1). Results: TPNS significantly inhibited the vascular intimal hyperplasia. TPNS significantly lowered the expression of PCNA, cyclinE, cyclinD1, FN and MMP-9. TPNS had no significant impacts on the expression of Col-I and TIMP-1. Conclusions: Our studies indicated that TSPN could inhibit vessel restenosis after vascular intimal injury, and its mechanisms may be related to the blockage of the excessive proliferation of VSMC, the reduction of ECM protein deposition in the endometrium, and the degradation of ECM protein. (C) 2009 Elsevier GmbH. All rights reserved.
摘要：
Angiogenesis in the infarct periphery can improve blood flow. Vascular endothelial growth factor (VEGF) has been considered a potential therapeutic target for stroke. Buyang Huanwu decoction (BYHWD) is a classic traditional formula in traditional Chinese medicine and is used to treat stroke; in addition, the promotion effects on VEGF protein expression have been confirmed. However, little is known about how BYHWD regulates angiogenesis, or about the effects of BYHWD on VEGF mRNA expression. For this reason, the present study measured microvessel density in rats with cerebral ischemia using immunohistochemistry. In addition, VEGF expression was measured by reverse-transcription polymerase chain reaction and enzyme-linked immunosorbent assay to determine the effects of BYHWD on angiogenesis and VEGF expression in rats with cerebral ischemia. Results demonstrated that microvessel density, as well as VEGF mRNA and protein expression, increased after 7 and 14 days of BYHWD treatment, which suggests that BYHWD promoted angiogenesis following cerebral ischemia and upregulated VEGF mRNA and protein expression in ischemic cerebral regions
摘要：
Atherosclerosis is characterized by a chronic inflammatory condition that involves numerous cellular and molecular inflammatory components. A wide array of inflammatory mediators, such as cytokines and proteins produced by macrophages and other cells, play a critical role in the development and progression of the disease. ATP-binding membrane cassette transporter A1 (ABCA1) is crucial for cellular cholesterol efflux and reverse cholesterol transport (RCT) and is also identified as an important target in antiatherosclerosis treatment. Evidence from several recent studies indicates that inflammation, along with other atherogenic-related mediators, plays distinct regulating roles in ABCA1 expression. Proatherogenic cytokines such as interferon (IFN)-γ and interleukin (IL)-1β have been shown to inhibit the expression of ABCA1, while antiatherogenic cytokines, including IL-10 and transforming growth factor (TGF)-β1, have been shown to promote the expression of ABCA1. Moreover, some cytokines such as tumor necrosis factor (TNF)-α seem to regulate ABCA1 expression in species-specific and dose-dependent manners. Inflammatory proteins such as C-reactive protein (CRP) and cyclooxygenase (COX)-2 are likely to inhibit ABCA1 expression during inflammation, and inflammation induced by lipopolysaccharide (LPS) was also found to block the expression of ABCA1. Interestingly, recent experiments revealed ABCA1 can function as an antiinflammatory receptor to suppress the expression of inflammatory factors, suggesting that ABCA1 may be the molecular basis for the interaction between inflammation and RCT. This review aims to summarize recent findings on the role of inflammatory cytokines, inflammatory proteins, inflammatory lipids, and the endotoxin-mediated inflammatory process in expression of ABCA1. Also covered is the current understanding of the function of ABCA1 in modulating the immune response and inflammation through its direct and indirect antiinflammatory mechanisms including lipid transport, high-density lipoprotein (HDL) formation and apoptosis.
作者：
He Qing-Zhi;Tuo Qin-Hui;Zeng Huai-Cai;Zhu Bing-Yang;Rang Wei-Qing;...
期刊：
生物化学与生物物理进展,2010年37(8):881-890 ISSN：1000-3282
通讯作者：
Tang Xiao-Qing
作者机构：
[Zhu Bing-Yang; Tuo Qin-Hui; Liao Duan-Fang; He Qing-Zhi] Univ S China, Prov Key Lab Pharmacoprotom, Inst Pharm & Pharmacol, Hengyang 421001, Peoples R China.;[Tang Xiao-Qing] Univ S China, Dept Physiol, Sch Med, Hengyang 421001, Peoples R China.;[Liao Duan-Fang] Hunan Univ Chinese Med, Dept Tradit Chinese Diagnot, Sch Pharm, Changsha 410208, Hunan, Peoples R China.;[Rang Wei-Qing; Zeng Huai-Cai] Univ S China, Sch Publ Hlth, Hengyang 421001, Peoples R China.
通讯机构：
[Tang Xiao-Qing] U;Univ S China, Dept Physiol, Sch Med, Hengyang 421001, Peoples R China.
关键词：
Daxx;Ox-LDL;macrophage;apoptosis;caveolin-1
摘要：
To explore whether Daxx mediates oxidized low-density lipoprotein (Ox-LDL)-induced cholesterol accumulation and apopotosis in macrophage and the underlying molecular mechanisms, intracellular lipid droplets and lipid levels were assayed by oil red O staining and high performance liquid chromatography (HPLC), respectively, the apoptotic effect of RAW264.7 cells induced by Ox-LDL was analyzed by flow cytometric analysis and acridine orange/ethidium bromide (AO/EB) staining, the mRNA expressions of Daxx was quantified by Real time RT- PCR, the protein expression of caveolin-1 was detected by Western-blotting, Daxx-specific small interfering RNA(Daxx siRNA) was transfected to RAW264.7 cell by lipofectamin. Ox-LDL up-regulated the expression of Daxx mRNA, increased the accumulation of intercellular cholesterol in RAW264.7 macrophages, and induced the apoptosis of RAW264.7 macrophages. However, Ox-LDL-induced intercellular cholesterol accumulation and apoptosis in RAW264.7 cells was prevented by Daxx siRNA. Ox-LDL also induced caveolin-1 expression and this effect is significantly suppressed by Daxx siRNA. It can be concluded that Daxx mediates Ox-LDL-induced cholesterol accumulation and apoptosis in macrophages by up-regulating caveolin-1 expression. These findings provide an important demonstration that Daxx might be associated with the development of atherosclerosis.
摘要：
Cholesterol efflux from lipid-loaded cells is a key athero-protective event that counteracts cholesterol uptake. The imbalance between cholesterol efflux and uptake determines the prevention or development of atherosclerosis. Many proteins and factors participate in the cholesterol efflux event. However, there are currently no systematic models of reverse cholesterol transport (RCT) that include most RCT-related factors and events. On the basis of recent research findings from other and our laboratories, we propose a novel model of one center and four systems with coupling transportation and networking regulation. This model represents a common way of cholesterol efflux; however, the systems in the model consist of different proteins/factors in different cells. In this review, we evaluate the novel model in vascular smooth muscle cells (VSMCs) and macrophages, which are the most important original cells of foam cells. This novel model consists of 1) a caveolae transport center, 2) an intracellular trafficking system of the caveolin-1 complex, 3) a transmembrane transport system of the ABC-A1 complex, 4) a transmembrane transport system of the SR-B1 complex, and 5) an extracelluar trafficking system of HDL/Apo-A1. In brief, the caveolin-1 system transports cholesterol from intracellular compartments to caveolae. Subsequently, both ABC-A1 and SR-B1 complex systems transfer cholesterol from caveolae to extracellular HDL/Apo-A1. The four systems are linked by a regulatory network. This model provides a simple and concise way to understand the dynamic process of atherosclerosis.
作者机构：
[Zhu Bing-Yang; Luo Di-Xian; Xu Can-Xin; Wang Chun; Gao Zhi-Ping; Liao Duan-Fang] Univ S China, Life Sci Res Ctr, Inst Pharm & Pharmacol, Hengyang 421001, Peoples R China.;[Liao Duan-Fang] Hunan Univ Chinese Med, Dept Tradit Chinese Diagnost, Changsha 410208, Hunan, Peoples R China.
通讯机构：
[Liao Duan-Fang] U;Univ S China, Life Sci Res Ctr, Inst Pharm & Pharmacol, Hengyang 421001, Peoples R China.
作者机构：
[Li, Hua; Chen, Bei-Yang] Hunan Univ Tradit Chinese Med, Dept Histol & Embryol, Changsha, Hunan, Peoples R China.;[Deng, Chang-Qing; Zhang, Shu-Ping; Liang, Yan] Hunan Univ Tradit Chinese Med, Lab Pathophysiol, Changsha, Hunan, Peoples R China.;[Li, Hua; Luo, Xue-Gang] Cent S Univ, Xiangya Sch Med, Dept Anat & Neurobiol, Changsha, Hunan, Peoples R China.
通讯机构：
[Deng, Chang-Qing] H;Hunan Univ Tradit Chinese Med, Lab Pathophysiol, Changsha, Hunan, Peoples R China.
关键词：
Total saponins of Panax notoginseng;Cerebral ischemia-reperfusion;TUNEL;Caspase-1;Caspase-3;Caspase-8
摘要：
Ethnopharmacological relevance: Total saponins of Panax notoginseng (TSPN), main constituents extracted from Panax Notoginseng, a highly valued traditional Chinese medicine, have been shown to be an effective agent on cerebral infarction. Aim of the study: The effects of TSPN on apoptosis and expressions of caspase-1, caspase-3 and caspase-8 were studied after cerebral ischemia for 2 h followed by reperfusion for 46 h in rats. Materials and methods: Rats were subjected to transient middle cerebral artery occlusion (MCAO) model using the intraluminal thread. TSPN was administered intraperitoneally at 5 min before and 12 h, 24 h and 36 h after MCAO, respectively. Results: TSPN (at the dose of 25 mg/kg) significantly attenuated TUNEL-positive cells and reduced the expression of caspase-1 and caspase-3 compared to the model group, while it had no obvious effect on the expression of caspase-8. Conclusions: The neuroprotective effect of TSPN on focal ischemia may be related to inhibition of apoptosis and caspases activation. (c) 2008 Elsevier Ireland Ltd. All rights reserved.
摘要：
Aim of the study: The inhibitive effect of BuYang HuanWu Decoction (BYHWD) and its major components on vascular intimal hyperplasia and the expressions of cell cycle protein and extracellular matrix protein. Materials and methods: Sprague-Dawley rats were randomly divided into sham-operated, control, alkaloid, glycoside, BYHWD and atorvastatin groups. Rat aorta intima in all groups were injured by insesion of domestic balloon catheter into the aortae except sham-operated rats. Drugs were administrated orally from the second day after vascular injury and continued for 14 days. The injured segments of aortae were collected on the sixteenth day after operation to observe the morphological changes of vascular structure and to examine the expressions of proteins in vascular cells associated with cell cycle including proliferating cell nuclear antigen(PCNA), cyclinD(1) and cyclinE, and extracellular matrix(ECM) proteins including collagen I (Col-1) and fibronectin (FN), further to discover the involved biologically active substances and the potential mechanisms. Results: Alkaloid and glycosid isolated from BYHWD were more effective than BYHVVD in the inhibition of intimal hyperplasia and the expressions of PCNA, cyclinD(1), cychnE, Col-I and FN, suggesting that alkaloid and glycoside may be the main components of BYHWD responsible for the observed inhibition of excessive hyperplasia of vascular intima. Conclusions: The mechanism associated with the anti-hyperplasia activity of BYHVVD and its effective components may be related to the blockage of cell cycles of VSMC, and the inhibition of the ECM protein synthesis, even the increased degradation of ECM proteins. (C) 2009 Elsevier Ireland Ltd. All rights reserved.
