作者机构：
[Liao, Duan-fang; Choudhary, M. Iqbal; Shehla, Nuzhat; Li, Bin; Wang, Wei; Atta-ur-Rahman; Cao, Liang; Jian, Yuqing] Hunan Univ Chinese Med, TCM & Ethnomed Innovat & Dev Int Lab, Acad Atta Ur Rahman Belt & Rd Tradit Med Res Ctr, Sch Pharm, Changsha, Peoples R China.;[Choudhary, M. Iqbal; Shehla, Nuzhat; Wang, Wei; Atta-ur-Rahman] Univ Karachi, HEJ Res Inst Chem, Int Ctr Chem & Biol Sci, Karachi, Pakistan.;[Zhao, Jianping; Khan, Ikhlas A.] Univ Mississippi, Natl Ctr Nat Prod Res, Pharmaceut Sci Res Inst, University, MS 38677 USA.
通讯机构：
[Choudhary, MI ] H;Hunan Univ Chinese Med, TCM & Ethnomed Innovat & Dev Int Lab, Acad Atta Ur Rahman Belt & Rd Tradit Med Res Ctr, Sch Pharm, Changsha, Peoples R China.;Univ Karachi, HEJ Res Inst Chem, Int Ctr Chem & Biol Sci, Karachi, Pakistan.
作者机构：
[Ahmed, Rida; Wang, Wei; Jiang, Sai; Liu, Yang; Liu, Bin; Li, Bin; Xie, Qian; Daniyal, Muhammad; Liu, B] Hunan Univ Chinese Med, Sch Pharm, Innovat Mat Med Res Inst, TCM & Ethnomed Innovat & Dev Int Lab, Changsha 410208, Peoples R China.;[Liu, B; Wang, Zhou; Long, Ying; Liu, Bin] Hunan Univ, Coll Biol, Changsha 410082, Hunan, Peoples R China.
通讯机构：
[Liu, B ; Wang, W ; Liu, B] H;Hunan Univ Chinese Med, Sch Pharm, Innovat Mat Med Res Inst, TCM & Ethnomed Innovat & Dev Int Lab, Changsha 410208, Peoples R China.;Hunan Univ, Coll Biol, Changsha 410082, Hunan, Peoples R China.
期刊：
Journal of Ethnopharmacology,2021年267:113496 ISSN：0378-8741
通讯作者：
Zeng, Rong;Wang, W
作者机构：
Hunan Univ Chinese Med, TCM & Ethnomed Innovat & Dev Int Lab, Sch Pharm, Changsha 410208, Hunan, Peoples R China.;Hunan Univ Chinese Med, Innovat Mat Med Res Inst, Sch Pharm, Changsha 410208, Hunan, Peoples R China.;[Wang, Wei; Zeng, Rong] Hunan Univ Chinese Med, Sch Pharm, Changsha 410208, Peoples R China.
通讯机构：
[Wang, W ; Zeng, R] H;Hunan Univ Chinese Med, Sch Pharm, Changsha 410208, Peoples R China.
作者：
Li B.;Peng C.-Y.;Chen Y.-Y.;Duan S.-L.;Peng Y.-L.;...
期刊：
中草药,2021年52(2):335-340 ISSN：0253-2670
通讯作者：
Li, S.-X.;Wang, W.
作者机构：
[Danial M.; Li B.; Peng C.-Y.; Duan S.-L.; Wang W.; Peng Y.-L.] TCM and Ethnomedicine Innovation & Development International Laboratory, School of Pharmacy, Hunan University of Chinese Medicine, Changsha, 410208, China;[Chen Y.-Y.; Li S.-X.] Hunan Engineering Research Center of Bioactive Substance Discovery of Chinese Medicine, School of Pharmacy, Hunan University of Chinese Medicine, Changsha, 410208, China
通讯机构：
[Wang, W.] T;[Li, S.-X.] H;Hunan Engineering Research Center of Bioactive Substance Discovery of Chinese Medicine, China;TCM and Ethnomedicine Innovation & Development International Laboratory, China
作者机构：
[袁汉文; 吴倩; 王炜; 盛文兵; 彭彩云; 姚萌; 李斌] TCM and Ethnomedicine Innovation & Development International Laboratory, School of Pharmacy, Sino-Pakistan TCM & Ethnomedicine International Cooperation Base, Innovative Materia Medica Research Institute, School of Pharmacy, Hunan University of Chinese Medicine, Changsha, 410208, China;[王思成] Chu Kochen Honors College, Zhejiang University, Hangzhou, 310012, China
通讯机构：
[Li, B.; Peng, C.-Y.] T;TCM and Ethnomedicine Innovation & Development International Laboratory, China
期刊：
Sensors and Actuators B-Chemical,2021年329:129203 ISSN：0925-4005
通讯作者：
Liu, B;Wang, W
作者机构：
[Liu, Bin; Liu, B; Zhou, Hongyan; Tong, Chunyi; Luo, Ruxin] Hunan Univ, Coll Biol, Changsha 410082, Peoples R China.;[Danial, Muhammad; Qin, Yan; Wang, Wei; Yu, Huanghe; Xie, Qian] Hunan Univ Chinese Med, Sch Pharm, TCM & Ethnomed Innovat & Dev Int Lab, Changsha 410208, Peoples R China.;[Liu, Bin; You, Peidong] Ningxia Med Univ, NHC Key Lab Metab Cardiovasc Dis Res, Yinchuan 750004, Ningxia, Peoples R China.
通讯机构：
[Liu, B ; Wang, W ] H;Hunan Univ, Coll Biol, Changsha 410082, Peoples R China.;Hunan Univ Chinese Med, Sch Pharm, TCM & Ethnomed Innovat & Dev Int Lab, Changsha 410208, Peoples R China.
摘要：
Polynucleotide kinase (PNK) is critical for nucleic acid metabolism, as its overexpression can increase ROS production and activate the inflammation pathway, leading to chronic inflammation. In this study, we proposed a new fluorescence method for monitoring PNK in vitro, natural compounds screening and intracellular imaging. Quantitative detection depicted that the limit of detection (LOD) of PNK is 0.0021 U/mL in the linear range of 0.01 similar to 10 U/mL. Methodological evaluation showed that both of the selectivity and the inhibitory effect of ADP and EDTA on PNK activity with satisfactory results. Fluorescence imaging of inflammatory cells demonstrated the reliability of platform for capturing PNK change in situ regulated by inhibitor of natural compound NS17 isolated from Kadsura heterolita. Mechanism study showed that the inhibitor NS17 can reduce inflammation by scavenging intracellular ROS, killing inflammatory cells and down-regulating COX-2 expression, the processes of which are related with PNK activity. In summary, above results suggest that this new platform could monitor PNK activity in situ and screen inflammation inhibitors targeted on PNK.
作者：
Tasneem, Shumaila*;Yang, Yupei;Liu, Bin;Choudhary, M. Iqbal;Wang, Wei
期刊：
Revista Brasileira de Farmacognosia,2021年31(1):51-58 ISSN：0102-695X
通讯作者：
Tasneem, Shumaila;Wang, W;Tasneem, S
作者机构：
[Tasneem, Shumaila; Liu, Bin; Yang, Yupei; Choudhary, M. Iqbal; Wang, Wei; Tasneem, S] Hunan Univ Chinese Med, Tradit Chinese Med & Ethnomed Innovat & Dev Int L, Tradit Chinese Med & Ethnomed Res Ctr, Innovat Drug Res Inst,Sch Pharm, Changsha 410208, Peoples R China.;[Tasneem, Shumaila; Choudhary, M. Iqbal; Tasneem, S] Univ Karachi, Int Ctr Chem & Biol Sci, Dr Panjwani Ctr Mol Med & Drug Res, Karachi 75270, Pakistan.;[Liu, Bin] Hunan Univ, State Key Lab, Hunan Prov Key Lab Plant Funct Genom & Dev Regula, Coll Biol, Changsha 410082, Peoples R China.
通讯机构：
[Wang, W ; Tasneem, S] H;[Tasneem, S ] U;Hunan Univ Chinese Med, Tradit Chinese Med & Ethnomed Innovat & Dev Int L, Tradit Chinese Med & Ethnomed Res Ctr, Innovat Drug Res Inst,Sch Pharm, Changsha 410208, Peoples R China.;Univ Karachi, Int Ctr Chem & Biol Sci, Dr Panjwani Ctr Mol Med & Drug Res, Karachi 75270, Pakistan.
摘要：
Breast cancer is an invasive malignant neoplasm; however, an increasing number of naturally occurring chemical compounds have been identified as potent cytotoxic agents. Kadsura coccinea (Lem.) A.C.Sm., Schisandraceae, is a medicinally important Chinese plant. Schisandronic acid, a cycloartane triterpenoid, is a major cytotoxic compound from this plant. The mechanisms behind the cytotoxic potential of schisandronic acid against a human breast carcinoma cell line were evaluated by employing cell viability assay using MTT and western blot analysis. Antioxidant capacity was measured by employing luminol- and lucigenin-enhanced chemiluminescence assays. Schisandronic acid was able to induce cell apoptosis in MCF-7 cells in a time-dependent manner. This triterpenoid significantly reduced cell viability in a concentration-dependent manner with an IC50 value of 8.06 +/- 1.119 mu M. The mechanisms might be mediated through upregulation of the expression of active caspase-3, and cleavage of poly (ADP-ribose) polymerase. Furthermore, schisandronic acid was investigated for its antioxidant effects and shown reduced generation of reactive oxygen species.
通讯机构：
[Liu, B ; Wang, W ; Qin, Y] H;Hunan Univ Chinese Med, Sch Pharmay, TCM, Changsha 410208, Hunan, Peoples R China.;Hunan Univ Chinese Med, Sch Pharmay, Ethnomed Innovat & Dev Int Lab, Changsha 410208, Hunan, Peoples R China.;Hunan Univ, Coll Biol, Changsha 410082, Hunan, Peoples R China.
关键词：
Glutathione S-transferases (GSTs);Bi-functional fluorescence probe;Natural drug screening;GSTs inhibitor
摘要：
Glutathione S-transferase (GSTs), as an important target for drug screening, is closely associated with physiological and pathological process regulation. Hence, effective monitoring of GSTs activity is benificial for GSTs-induced diseases diagnosis and therapeutic drugs discovery. Resorufin-typed probes with high sensitivity, excellent selectivity, and low cytotoxicity have become a powerful tool for target monitoring. In this study, a bifunctional fluorescent probe, resorufin-typed probe (RP) with resorufin as a fluorophore was designed as the GSTs substrate and drug screening tool. GSTs can trigger significant fluorescence signal enhancement of RP by catalyzing the combination of glutathione (GSH) and its recognition site in RP. Meaningfully, the change of fluorescence signal positively correlated with GSTs activity can be used to monitor the regulatory effect of natural drugs on GSTs. Based on the axis relation of "drug-GSTs activity-fluorescence signal", this powerful probe was successfully employed for visual and rapid screening GSTs-targeted natural compounds in vitro by monitoring the change of GSTs activity. Natural compound 1, 2 and 7 (C1, C2 and C7) were screened as the GSTs inhibitors, and C7 was helpful for enhancing the sensitivity of cisplatin in cancer cells. Overall, the designed natural drug screening strategy based on bi-functional fluorescence probe demonstrated its hopeful application to drug rapid discovery.
作者机构：
[袁汉文; 彭彩云; 翦雨青; 周旭东; 李斌; 盛文兵; 龚力民; 王炜] Sino-Pakistan TCM and Ethnomedicine Research Center, Innovative Materia Medica Research Institute, School of Pharmacy, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;[何述金] Hunan Xinhui Pharmaceutical Co., Ltd., Changsha, Hunan 410000, China;[刘昌孝] Tianjin Institute of Pharmaceutical Research, Tianjin 300193, China;[周准] Sino-Pakistan TCM and Ethnomedicine Research Center, Innovative Materia Medica Research Institute, School of Pharmacy, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China<&wdkj&>Hunan Xinhui Pharmaceutical Co., Ltd., Changsha, Hunan 410000, China
通讯机构：
[Wang Wei] S;[Liu Chang-Xiao] T;Tianjin Institute of Pharmaceutical Research, Tianjin 300193, China<&wdkj&>Sino-Pakistan TCM and Ethnomedicine Research Center, Innovative Materia Medica Research Institute, School of Pharmacy, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China
摘要：
<jats:sec>
<jats:title>Background:</jats:title>
<jats:p>Intramedullary fixation a standard surgical technique for long bone meta/diaphyseal fractures. There were many difficulties in removal of the intractable intramedullary device. The authors reported a new technique to remove the intractable intramedullary nail by using a self-made connecting device.</jats:p>
</jats:sec>
<jats:sec>
<jats:title>Methods:</jats:title>
<jats:p>The subject underwent removal of the intramedullary nail using a self-made connecting device, the core components of which were a caudal connecting rod and a sliding hammer in the common intramedullary nail removal device, and the auxiliary device was mainly a clinically commonly used Kirschner wire (K-wire; diameter 1.5–2.5 mm). In technical procedure, the key point was the connection between the k-wire and the intramedullary device, according to the specific conditions of the intramedullary device.</jats:p>
</jats:sec>
<jats:sec>
<jats:title>Results:</jats:title>
<jats:p>From 2012 to 2017, a total of 10 cases of intractable intramedullary devices were taken out using this self-made connection device, including 7 cases of tibial intramedullary nails, 1 case of femoral nail, and 1 case of tibial elastic nail. The technique provided satisfactory results, no infection or re-fracture occurred after the.</jats:p>
</jats:sec>
<jats:sec>
<jats:title>Conclusion:</jats:title>
<jats:p>The self-made connecting device may provide new technique for more surgeons in the face of intractable intramedullary device.</jats:p>
</jats:sec>
作者：
Wang X.-J.;Xie Q.;Liu Y.;Lu S.-Y.;Muhammad D.;...
期刊：
中草药,2020年51(7):1831-1838 ISSN：0253-2670
通讯作者：
Wang, W.
作者机构：
[Mao Y.; Yu Y.; Liang N.; Wang X.-J.] Hunan Food and Drug Vocational College, Changsha, 410208, China;[Wang W.; Liu Y.; Gong L.-M.; Xie Q.; Duan S.-L.; Muhammad D.; Li B.] TCM and Ethnomedicine Innovation & Development International Laboratory, Innovative Drug Research Institute, Sino-pakisitan TCM & Ethnomedicine Research International Cooperation Base (Hunan Province), School of Pharmacy, Hunan University of Chinese Medicine, Changsha, 41020, China;Department of Phamacy, Liuzhou Traditional Chinese Medical Hospital Guangxi Zhuang Autonomous Region, Liuzhou, 545026, China;[Lu S.-Y.] TCM and Ethnomedicine Innovation & Development International Laboratory, Innovative Drug Research Institute, Sino-pakisitan TCM & Ethnomedicine Research International Cooperation Base (Hunan Province), School of Pharmacy, Hunan University of Chinese Medicine, Changsha, 41020, China, Department of Phamacy, Liuzhou Traditional Chinese Medical Hospital Guangxi Zhuang Autonomous Region, Liuzhou, 545026, China
通讯机构：
[Wang, W.] T;TCM and Ethnomedicine Innovation & Development International Laboratory, China
作者机构：
[Liu, Bin; Liu, B; Xiao, Chang; Zhong, Guowei; Tong, Chunyi] Hunan Univ, Coll Biol, Changsha 410082, Peoples R China.;[Yang, Yuejun; Zhong, Xianghua; Yang, Xiaoping] Hunan Normal Univ, Sch Med, Changsha 410125, Peoples R China.;[Liu, Xu; Wang, Wei] Hunan Univ Chinese Med, Sch Pharm, TCM & Ethnomed Innovat & Dev Int Lab, Changsha 410208, Peoples R China.;[Liu, Bin] Ningxia Med Univ, Sch Basic Med Sci, Dept Physiol & Pathophysiol, NHC Key Lab Metab Cardiovasc Dis Res, Yinchuan 750004, Ningxia, Peoples R China.
通讯机构：
[Liu, B ; Wang, W ] H;Hunan Univ, Coll Biol, Changsha 410082, Peoples R China.;Hunan Univ Chinese Med, Sch Pharm, TCM & Ethnomed Innovat & Dev Int Lab, Changsha 410208, Peoples R China.
