摘要：
According to the difference in temperature, thermotherapy can be divided into thermal ablation and mild hyperthermia. The main advantage of thermal ablation is that it can efficiently target tumors in situ, while mild hyperthermia has a good inhibitory effect on distant metastasis. There are some similarities and differences between the two therapies with respect to inducing anti-tumor immune responses, but neither of them results in sustained systemic immunity. Malignant tumors (such as breast cancer, pancreatic cancer, nasopharyngeal carcinoma, and brain cancer) are recurrent, highly metastatic, and highly invasive even after treatment, hence a single therapy rarely resolves the clinical issues. A more effective and comprehensive treatment strategy using a combination of hyperthermia and immune checkpoint inhibitor (ICI) therapies has gained attention. This paper summarizes the relevant preclinical and clinical studies on hyperthermia combined with ICI therapies and compares the efficacy of two types of hyperthermia combined with ICIs, in order to provide a better treatment for the recurrence and metastasis of clinically malignant tumors.
期刊：
Dyes and Pigments,2022年207:110742 ISSN：0143-7208
通讯作者：
Ruicheng Hu
作者机构：
[Dai, Aiguo] Hunan Normal Univ, Hunan Prov Peoples Hosp, Dept Resp Med, Affiliated Hosp 1, Changsha 410016, Peoples R China.;[Wu, Zhaoli] Hunan Univ Chinese Med, Med Sch, Dept Resp Dis, Changsha 410208, Peoples R China.;[Shi, Zeya] Hunan Normal Univ, Hunan Prov Peoples Hosp, Dept Integrated TCM & Western Med, Affiliated Hosp 1, Changsha 410016, Peoples R China.;[Hu, Ruicheng] Hunan Normal Univ, Hunan Prov Peoples Hosp, Dept Sci Res, Affiliated Hosp 1, Changsha 410016, Peoples R China.;[Hu, Ruicheng] Hunan Normal Univ, Hunan Prov Peoples Hosp, Dept Resp Med, Affiliated Hosp 1, Gu Han Rd 89, Changsha 410016, Hunan, Peoples R China.
通讯机构：
[Ruicheng Hu] D;Department of Respiratory Medicine, Hunan Provincial People's Hospital/The First Affiliated Hospital of Hunan Normal University, Changsha, 410016, China
关键词：
Fluorescent imaging;Rhodamine chemosensor;ClO-Imaging;Fluorescence turn on
摘要：
HClO is recognized as an indicator for phagocytosis and inflammatory response. Excess HClO ruptures lysosomes and leads to cell apoptosis, which has been proposed as a cure for cancer treatment. As a consequence, the real-time and in situ cell imaging for HClO is highly appealed in the field of anticancer pharmacy. In this work, a series of silane-modified rhodamine fluorescers with various functional groups were designed, hoping to find a fluo-rescer with near IR emission for bio-imaging in live cells, along with unique sensing response towards ClO- over competing species. These silane-modified rhodamine fluorescers were carefully characterized, their photo -physical parameters and responses towards ClO- and competing species were compared systematically. An optimal probe showed emission quantum yield of 0.26 with emission wavelength at 681 nm. Emission "turn-on" effect was observed, with calibration equation of F = 0.720*[ClO-]2+40.047*[ClO-]+54.982, R2 = 0.999, and the limit of detection (LOD) was calculated as 0.03 mu M. Corresponding sensing mechanism was revealed as an HClO-mediated cyclization reaction. Fluorescence imaging for Hela cells and A549 lung cancer cells was per-formed. The lysosome-targetable feature was confirmed.
摘要：
BACKGROUND Wound healing is a dynamic and complex process that is regulated by a variety of factors and pathways. This study sought to identify the mechanisms of the four-herb Chinese medicine ANBP in enhancing wound repair. MATERIAL AND METHODS By comparing the group treated with ANBP for 6 h (Z6h) with the corresponding control group (C6h), we used the new high-throughput differential acetylation proteomics method to explore the mechanism of ANBP treatment and analyse and identify new targets of ANBP for promoting wound healing. RESULTS ANBP promoted skin wound healing in mice; the wound healing process was accelerated and the wound healing time was shortened (P<0.05). The upregulated proteins were distributed mostly in the mitochondria to nuclear respiratory chain complexes and cytoplasmic vesicles. The dominant pathways for upregulated proteins were fatty acid metabolism, pyruvate metabolism, and tricarboxylic acid cycle. Pdha1 was upregulated with the most acetylation sites, while the downregulated Ncl, and Pfkm were most acetylated. CONCLUSIONS The findings from our study showed that ANBP improved cell aerobic respiration through enhanced glycolysis, pyruvic acid oxidative decarboxylation, and the Krebs cycle to produce more ATP for energy consumption, thus accelerating wound repair of skin.
期刊：
Frontiers in Molecular Neuroscience,2022年14:785938 ISSN：1662-5099
通讯作者：
Wang, Yang
作者机构：
[Wang, Yang; Luo, Weikang; Yang, Zhaoyu] Cent South Univ, Xiangya Hosp, Inst Integrat Med, Dept Integrated Chinese & Western Med, Changsha, Peoples R China.;[Wang, Yang; Luo, Weikang; Yang, Zhaoyu] Cent South Univ, Xiangya Hosp, Natl Clin Res Ctr Geriatr Disorders, Changsha, Peoples R China.;[Zhang, Wei] Hunan Univ Chinese Med, Coll Integrated Tradit Chinese & Western Med, Changsha, Peoples R China.;[Zhou, Dan] Hunan Univ Chinese Med, Period Off, Changsha, Peoples R China.;[Guo, Xiaohang] Hunan Univ Chinese Med, Med Sch, Changsha, Peoples R China.
通讯机构：
[Wang, Yang] C;Cent South Univ, Xiangya Hosp, Inst Integrat Med, Dept Integrated Chinese & Western Med, Changsha, Peoples R China.;Cent South Univ, Xiangya Hosp, Natl Clin Res Ctr Geriatr Disorders, Changsha, Peoples R China.
摘要：
BACKGROUND: Pulmonary hypertension (PH) is a progressive disorder lacking a validated and effective therapy which characterized by elevated pulmonary arterial pressure, vascular remodeling and eventual death. FDA approved sildenafil is being used as a first-line drug for PH, however, neither survival rates nor quality of life have been improved because of side effects and patient noncompliance. Thus, the exploration of novel therapeutic drugs is urgently needed. Astragaloside IV (ASIV) exhibits a protective effect on HPH, but its mechanisms of action is unclear. HYPOTHESIS: CD4+T cell subsets, Tfh and Tfr cells, may contribute to the development of chronic hypoxia-induced PH (HPH). We hypothesized that ASIV could effectively ameliorates pulmonary vascular remodeling of HPH by restraining the Tfh cell response and expanding Tfr cell response. METHODS AND RESULTS: HPH mice model was established by exposure to chronic hypoxia for 21 days. Mice were randomly assigned to six groups: NaCl group, model group, SN group (100mg/kg of sildenafil), low-dose group (20mg/kg of ASIV), medium-dose group (40mg/kg of ASIV) and high-dose group (80mg/kg of ASIV). Primary culture and identification of distal pulmonary artery smooth muscle cells (PASMCs) in mice were established. Here, we demonstrated that ASIV treatment could significantly ameliorate the increase of mean PAP, RV/ (LV+S) ratio and PAMT in HPH mice. ASIV inhibited Tfh cell differentiation and IL-21 production, but promoted Tfr cell differentiation and TGF-β, IL-10 production. Chronic hypoxia promoted germinal center B cell responses, which inhibited by ASIV. ASIV regulated Tfh and Tfr cell differentiation by inhibiting the phosphorylation of mTOR signaling pathway, and the effect of ASIV-H was better than that observed in the SN group. ASIV inhibited the proliferation, migration and adhesion of PASMCs in vitro. Moreover, ASIV significantly downregulated the protein level of RhoA and upregulated the protein level of p27 in PASMCs under hypoxic condition. CONCLUSION: Collectively, ASIV may regulate Tfh and Tfr cell responses to subsequently repress pulmonary vascular remodeling and hypoxic pulmonary hypertension.
作者机构：
[Wang, Xiaoqi; Ning, Yi; Lu, Fangguo; Hu, Jue; Xiao, Rong; Liu, Shiwu] Hunan Univ Chinese Med, Dept Microbiol, Med Sch, Changsha 410208, Hunan, Peoples R China.;[Li, Ling] Hunan Univ Chinese Med, Expt Ctr Mol Biol, Med Sch, Changsha 410208, Hunan, Peoples R China.
通讯机构：
[Yi Ning; Fangguo Lu] D;Department of Microbiology, The Medicine School of Hunan University of Chinese Medicine, Changsha, Hunan, 410208, People's Republic of China<&wdkj&>Department of Microbiology, The Medicine School of Hunan University of Chinese Medicine, Changsha, Hunan, 410208, People's Republic of China
期刊：
Microbes and Infection,2022年24(8):104998 ISSN：1286-4579
通讯作者：
Ling, Li
作者机构：
[Huang, Jiawang; Ma, Xinyue; Zhu, Mengchen; Chen, Yulu] Hunan Univ Chinese Med, Coll Integrated Tradit Chinese & Western Med, Xueshi Rd 300, Changsha 410208, Hunan, Peoples R China.;[Lu, Fang-guo; Zhang, Bo; Hu, Jue; Cheng, Lijuan] Hunan Univ Chinese Med, Dept Microbiol, Med Sch, 300 Rd, Changsha, Hunan, Peoples R China.;[Li, Ling; Liu, Weirong] Hunan Univ Chinese Med, Coll Tradit Chinese Med, 300 Rd, Changsha, Hunan, Peoples R China.
通讯机构：
[Ling, Li] T;The College of Traditional Chinese Medicine, Hunan University of Chinese Medicine, 300 Road, Yuelu District, Changsha, Hunan Province, China. Electronic address:
摘要：
Acute lung injury (ALI) is characterized by tissue damage that leads to pulmonary epithelial membrane dysfunction and macrophage activation. Currently however, the exact mechanism by which the initial mediators of mouse lung epithelial (MLE-12) cells induce inflammation remines unclear. We constructed co-culture systems of MLE-12 cells with mouse macrophage cells RAW246.7 which were realized by the supernatant and Transwell chamber. In previous study, we successfully constructed an influenza A virus-induced MLE-12 cells model. Extracellular Vesicles (EVs) from cells supernatant were isolated by differential ultracentrifugation and confirmed by transmission electron microscopy. High-throughput sequencing results showed that MLE-12 cells stimulated by influenza A virus had higher level of miR-1249-5p. The results were validated by RT-qPCR analysis. The research aimed to investigate the roles and mechanisms of miR-1249-5p in ALI. RAW246.7 cells were transfected with miR-1249-5p mimic/inhibitor. The concentrations of TNF-alpha, IL-6 were determined by ELISA and the uptake of EVs was monitored by confocal laser scanning microscope. Western blotting detected changes in the SLC4A1 and NF-kappa B signaling pathway. The results indicated that miR-1249-5p played an important role in ALI, and further investigation of its target gene SLC4A1 and NF-kappa B signaling pathway provides ideas for new therapeutic targets and strategies for ALI. (C) 2022 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.
作者机构：
[Chen, Kaiqin; Wang, Xiaoqi; Ning, Yi; Lu, Fangguo; Xiong, Tao; Hu, Jue; Wei, Ke; Xiao, Rong] Hunan Univ Chinese Med, Sch Med, Changsha 410128, Peoples R China.;[Chen, Shanquan] Chinese Univ Hong Kong, Sch Humanities & Social Sci, Shenzhen 518172, Peoples R China.;[Lu, Fangguo] Hunan Univ Chinese Med, Affiliated Hosp 1, Changsha 410021, Peoples R China.;[Lu, Fangguo] Hunan Univ Chinese Med, Med Coll, Changsha 410128, Peoples R China.
通讯机构：
[Shanquan Chen; Fangguo Lu] S;School of Humanities and Social Sciences, The Chinese University of Hong Kong, Shenzhen, 518172, China<&wdkj&>School of Medicine, Hunan University of Chinese Medicine, Changsha, 410128, China<&wdkj&>The First Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, 410021, China
摘要：
Xiaoqu is a saccharifying and alcohol-producing, flavoring agent used in brewing Chinese rice wine (CRW). We combined high-throughput sequencing with headspace solid-phase microextraction-mass spectrometry-gas phase ion migration spectrometry to identify the microbial communities of four Xiaoqu rice wines collected from Xinhua County, and the flavoring substances of CRWs brewed with them. The most prevalent microorganisms at the genus level were Pediococcus, Enterobacter, Leuconostoc, Weissella, Staphylococcus, Pantoea, Glutamicibacter, Kosakonia, Lactobacillus, Rhizopus, and Mucor. We detected 43 volatile flavoring esters, alcohols, aldehydes, acids, and ketones in the Xiaoqu CRWs. We identified significant positive correlations between Pediococcus/Weissella/ Lactobacillus and ethyl butyrate, Pantoea/Kosakonia and 3-methyl-1-butanol/2-methyl-1-propanol, Lactococcus/ Streptococcus and ethyl lactate, and Apibacter/Rosenbergiella and ethyl acetate/3-methyl-1-butanol. Relationships between Pediococcus and Lactobacillus/Streptococcus, Rhizopus and Weissella, Mucor, and Leuconostoc, Hydropichia and almost all dominant bacterial genera, as well as Rhizopus and five other fungal genera were synergistic. Carbohydrate, coenzyme, and amino acid metabolism might be the main metabolic processes of dominant bacteria in Xiaoqu. We clarified the dominant microbial community structures of Xinhua Xiaoqu, characterized the flavor compounds in CRW and the relationships between microbiota and flavor. Our findings provide reference data for future flavor-based screens of beneficial bacterial and fungal strains to improve the quality of Xiaoqu CRW.
摘要：
BACKGROUND Ovarian cancer has the highest mortality of gynecological cancers worldwide. The aim of this study was to identify the role of tripterine against ovarian cancer. MATERIAL AND METHODS GSE18520 and GSE12470 data sets were downloaded from the GEO database. WGCNA was used to analyze gene modules and hub genes related to ovarian cancer. These hub genes were intersected with tripterine targets, and GO and KEGG enrichment analyses were performed. HPA and GEPIA determined the expression of tripterine anti-ovarian hub genes in tumor tissues. Kaplan-Meier plotter was used to explore the role of hub genes in ovarian cancer prognosis. AutoDock was used to conduct molecular docking of tripterine and hub genes to observe whether the combination was stable. RESULTS By differential analysis of gene expression and the construction of WGCNA co-expression network, 5 hub genes, ARHGAP11A, MUC1, HBB, RUNX1T1, and FUT8, were screened by module gene screening. Seven biological processes and 20 KEGG-related pathways were obtained by gene enrichment. The expression of tripterine anti-ovarian hub genes ARHGAP11A, MUC1, and FUT8 were obtained by HPA and GEPIA. Using Kaplan-Meier plotter, the survival of ovarian cancer was negatively correlated with ARHGAP11A, MUC1, and FUT8. Molecular docking showed the combination of tripterine and FUT8 was most stable, having the greatest potential role. CONCLUSIONS Tripterine may be involved in megakaryocyte development and platelet production through potential genes ARHGAP11A, MUC1, HBB, RUNX1T1, and FUT8 and may have an anti-ovarian cancer effect in immune factors signaling, transporting and exchanging oxygen pathways, and autophagy pathways, through these 5 key genes.
期刊：
Frontiers in Physiology,2022年13:2403 ISSN：1664-042X
作者机构：
[Mei, Si] Hunan Univ Chinese Med, Fac Med, Dept Physiol, Changsha, Hunan, Peoples R China.;[Tian, Xuefei; Meng, Yuelin; Chen, Yating; Guo, Yinmei; Deng, Zhe; Mei, Si; Ning, Dimin] Hunan Univ Chinese Med, Hunan Key Lab Translat Res Formulas & Zheng Tradit, Changsha, Hunan, Peoples R China.;[Tian, Xuefei; Wang, Ruoyu; Meng, Yuelin; Chen, Yating; Deng, Zhe; Ning, Dimin] Hunan Univ Chinese Med, Coll Integrated Chinese & Western Med, Dept Internal Med, Changsha, Hunan, Peoples R China.;[Fan, Xingxing] Macau Univ Sci & Technol, Macau Inst Appl Res Med & Hlth, State Key Lab Qual Res Chinese Med, Macau, Peoples R China.;[Wang, Ruoyu] Hunan Univ Chinese Med, Dept Liver Dis, Hosp 1, Changsha, Hunan, Peoples R China.
关键词：
Gut Microbiota;Dysbiosis;colorectal cancer;Hepatocellular Carcinoma;Carcinogenesis
摘要：
Gastrointestinal cancer may be associated with dysbiosis, which is characterized by an alteration of the gut microbiota. Understanding the role of gut microbiota in the development of gastrointestinal cancer is useful for cancer prevention and gut microbiota-based therapy. However, the potential role of dysbiosis in the onset of tumorigenesis is not fully understood. While accumulating evidence has demonstrated the presence of dysbiosis in the intestinal microbiota of both healthy individuals and patients with various digestive system diseases, severe dysbiosis is often present in patients with digestive system cancer. Importantly, specific bacteria have been isolated from the fecal samples of these patients. Thus, the association between dysbiosis and the development of digestive system cancer cannot be ignored. A new model describing this relationship must be established. In this review, we postulate that dysbiosis serves as the first hit for the development of digestive system cancer. Dysbiosis-induced alterations, including inflammation, aberrant immune response, bacteria-produced genotoxins, and cellular stress response associated with genetic, epigenetic, and/or neoplastic changes, are second hits that speed carcinogenesis. This review explains the mechanisms for these four pathways and discusses gut microbiota-based therapies. The content included in this review will shed light on gut microbiota-based strategies for cancer prevention and therapy.
期刊：
Revista Brasileira de Farmacognosia,2022年32(3):455-459 ISSN：0102-695X
通讯作者：
Ning, Yi;Lu, FG
作者机构：
[Ning, Yi; Wang, Xiaoqi; Lu, Fangguo; Liu, Shiwu; Lu, FG] Hunan Univ Chinese Med, Dept Microbiol, Med Sch, Changsha 410208, Hunan, Peoples R China.;[Zhao, Cheng; Zhang, Xianggang] Hunan Univ Chinese Med, Dept Integrated Tradit Chinese & Western Med, Med Sch, Changsha 410208, Hunan, Peoples R China.
通讯机构：
[Ning, Y; Lu, FG ] H;Hunan Univ Chinese Med, Dept Microbiol, Med Sch, Changsha 410208, Hunan, Peoples R China.
关键词：
Infectious disease;Drug-resistant bacteria;Monomer of traditional Chinese medicine;Anti-biofilm agents
摘要：
This study investigated the effect and possible mechanism of berberine on biofilm formation by methicillin-resistant Staphylococcus aureus. Compared with chloramphenicol, berberine showed a significantly stronger inhibitory effect on the growth of methicillin-resistant Staphylococcus aureus, and the minimum inhibition concentration and minimum bactericidal concentration for berberine were 0.05 mg/ml and 0.1 mg/ml, respectively (p < 0.05). In addition, berberine significantly impaired biofilm formation and reduced biofilm thickness in a dose-dependent manner (p < 0.05). Furthermore, the results of quantitative real-time polymerase chain reaction revealed that, at concentrations ranging from 20 to 120 mu g/ml, berberine inhibited the expression of agrA, agrD, agrB, and agrC (p < 0.05). The molecular regulatory mechanism of berberine inhibition may be obtained by its downregulation of the expression of agrA, agrD, agrB, and agrC, thereby inhibiting biofilm formation by methicillin-resistant Staphylococcus aureus. These findings demonstrate that berberine has a great potential as a quorum sensing inhibitor against biofilm-associated infections.
摘要：
Background: Astragalus and Panax notoginseng are significant traditional Chinese medicines for treating ischemic stroke, with astragaloside IV (AST IV) and Panax notoginseng saponins (PNS) being the major effective compounds, respectively. These compounds can also be used in combination. We have previously shown that AST IV and PNS have an antagonistic effect on cerebral ischemia/reperfusion (I/R) injury, and the combination of these two drugs can elevate this effect; unfortunately, AST IV and PNS cannot easily enter the brain tissues through the blood brain barrier (BBB). Previous studies have confirmed that the combination of borneol with other agents could promote the penetration of the drug components through the BBB. However, it remains unclear whether borneol can promote entry of the active components of AST IV and PNS into the brain tissues and enhance their effect against cerebral ischemia. Objective: This study aimed to investigate the effects of a combination of borneol with AST IV and PNS against I/R injury and explore the mechanisms of borneol-promoting penetration of drug components into the BBB based on the drug transport of brain tissues. Methods: A rat model of focal cerebral I/R injury was established, and drugs, including borneol, AST IV, and PNS, as well as their combinations were intragastrically administered. Subsequently, drug efficacy was assessed, and the condition of AST IV and PNS active components (Rg1, Rb1, R1) delivered into the brain was analyzed. Moreover, BBB permeability was determined, and the expression of related drug transporters and their genes were evaluated. Results: After treatment with borneol, AST IV, PNS, AST IV+PNS, and borneol+AST IV+PNS after cerebral I/R, the neurological function deficit scores, cerebral infarct rate, and brain water content markedly decreased. The effects of the three-drug-combination were better than those of the drugs used alone and those of AST IV+PNS. Moreover, after I/R in rats, AST IV and the components of PNS (Rg1, Rb1, R1) were mainly found in the cerebral cortex and in the cerebellum, respectively, when used alone. Borneol combined with AST IV and PNS increased the contents of AST IV, Rb1, Rg1, and R1 in the cerebral cortex and in the cerebellum, thus, promoting the enrichment of active components to the cerebral cortex, especially to the affected side. In addition, following I/R, diffuse distribution of lanthanum particles in the basement membrane, intercellular and intracellular locations of rat brain tissues indicated BBB destruction and increase in permeability, which were alleviated in each drug group. The effects of borneol combined with AST IV and PNS were stronger than those of the drug single-used and those of the AST IV+PNS group. Finally, the expression of effluent transporters (ET) and their genes, including P-glycoprotein (P-gp), multidrug resistance protein (MRP)-1, MRP-2, MRP-4, and MRP-5 in brain tissues, strikingly increased after I/R. Borneol remarkedly down-regulated the protein expression of P-gp, MRP-2, and MRP-4 in the brain, whereas PNS down-regulated MRP-4 and MRP-5 protein expression. AST IV, AST IV+PNS, and bornoel+AST IV+PNS effectively decreased the expression of P-gp, MRP-2, MRP-4, and MRP-5 proteins. The effects of the three-drug combination were significantly greater than those of the drug single-used and AST IV+PNS groups. The expression of each ET gene manifested corresponding results. Meanwhile, PNS, AST IV+PNS, and bornoel+AST IV+PNS significantly inhibited the down-regulation of the uptake transporter organic anion transporting polypeptide (OATP)-2 expression, and the effect of bornoel+AST IV+PNS was stronger than that of other groups. Conclusion: After I/R, the brain tissues were injured, BBB permeability increased, expression of critical ET and their genes were markedly up-regulated, and the main uptake transporters were down-regulated. We propose that the combination of borneol, AST IV and PNS could enhance the effect against cerebral I/R injury and protect BBB integrity. The potential mechanism might be the delivery of AST IV and active components of PNS to the brain tissues after treatment in combination with borneol, which could be effectively promoted by downregulating the expression of ETs and up-regulating the expression of uptake transporters in the brain tissues. This study was the first to demonstrate that borneol combined with AST IV+PNS enhanced the effect against cerebral I/R injury through promoting the entry of AST and PNS active components to the brain tissues. Thus, this study proposes an instructive role in developing effective active ingredients combination of Chinese medicine with clear ingredients and synergistic effects in terms of the characteristic of borneol.
期刊：
Frontiers in Cellular and Infection Microbiology,2022年12:1207 ISSN：2235-2988
作者机构：
[Xie, Guozhen; Zheng, Tao] Hunan Univ Chinese Med, Sch Pharm, Changsha, Peoples R China.;[Deng, Na] Hunan Univ Chinese Med, Coll Tradit Chinese Med, Changsha, Peoples R China.;[Peng, Xinxin] Hunan Univ Chinese Med, Hosp 1, Changsha, Peoples R China.;[Zhang, Shuihan] Hunan Acad Chinese Med, Inst Chinese Mat Med, Changsha, Peoples R China.;[Tan, Zhoujin] Hunan Univ Chinese Med, Coll Med, Changsha, Peoples R China.
关键词：
Total glycosides of Qiwei Baizhu Powder;Gut Microbiota;Bile acid metabolism;Diarrhea;Bioactive component
摘要：
Qiwei Baizhu Powder (QWBZP) is a traditional Chinese medicine formula for treating diarrhea induced by various causes. It elicits an anti-diarrheal effect by regulating the gut microbiota (diversity, structure, and abundance). However, the contribution of different components in the QWBZP decoction to this effect remains unclear. In this study, we used the QWBZP decoction as a reference standard to investigate the effects of total glycosides (TGs) extracted from QWBZP decoction on the gut microbiota and bile acid metabolism in mice with antibiotic-associated diarrhea (AAD). The results of 16S rRNA gene sequencing and liquid chromatography-mass spectrometry (LC-MS) analysis showed that the effect of total glycosides of Qiwei Baizhu Powder (QWBZP-TG) on specific intestinal bacteria and bile acids was similar to that of the QWBZP decoction, but the intensity of this effect was more significant in the case of QWBZP-TG. The QWBZP decoction and QWBZP-TG promoted the proliferation of Lactobacillus and inhibited the growth of Proteus, Clostridium, Eubacterium, Facklamia, and Escherichia in AAD mice. They also increased the levels of deoxycholic acid and beta-muricholic acid and decreased those of taurocholate acid, tauro-alpha-muricholic acid, and tauro-beta-muricholic acid in AAD mice. Lactobacillus was the key bacterial genus responding to QWBZP-TG. Thus, this study provides novel insights into the bioactive components of QWBZP and their contribution to its effects.
作者机构：
[Li, Li; Chen, Dilu; Zhou, Xiaojiang; Qin, Li] Hunan Univ Chinese Med, Coll Pharm, Changsha 410208, Peoples R China.;[Yang, Shenghui; Wu, Zhihuang; Zhang, Meijun] Hunan Univ Chinese Med, Coll Med, Changsha 410208, Peoples R China.;[Yang, Fang] Changde Inst Food Inspection, Changde 415000, Peoples R China.;[Qin, Li] Hunan Univ Chinese Med, Lab Stem Cell Regulat Chinese Med & Its Applicat, Changsha 410208, Peoples R China.
通讯机构：
[Li Qin; Xiaojiang Zhou] A;Authors to whom correspondence should be addressed.<&wdkj&>Laboratory of Stem Cell Regulation with Chinese Medicine and Its Application, Hunan University of Chinese Medicine, Changsha 410208, China<&wdkj&>College of Pharmacy, Hunan University of Chinese Medicine, Changsha 410208, China<&wdkj&>Authors to whom correspondence should be addressed.<&wdkj&>College of Pharmacy, Hunan University of Chinese Medicine, Changsha 410208, China
关键词：
the sesquiterpene-rich extracts of Carpesium abrotanoides;sesquiterpene;anti-influenza A virus H1N1;TLR4/MyD88/NF-κB signal pathway
摘要：
Due to a high content of sesquiterpenes, Carpesium abrotanoides has been investigated to fully explore its health-promoting properties. Therefore, this work aimed to assess, for the first time, the anti-influenza A virus H1N1 potential of sesquiterpene-targeted fractions of the herb derived from C. abrotanoides. Five compounds, including four sesquiterpenes and one aldehyde, were isolated and identified from the sesquiterpene-rich extracts of C. abrotanoides (SECA), and the contents of three main sesquiterpenes in the SECA were determined. Furthermore, SECA showed a significant protective effect in the MDCK cells infected with influenza A virus (H1N1) in three different conditions: premixed administration, prophylactic administration, and therapeutic administration. SECA can significantly decrease the mRNA expressions of TLR4, MyD88, NF-kappa B, TNF-alpha, and IL-6, as well as the protein expressions of TLR4, MyD88, and NF-kappa B. This result suggests that SECA can resist the influenza A virus H1N1 through the TLR4/MyD88/NF-kappa B signal pathway.
摘要：
There have been many literatures reporting the binding performance of transition metal complexes towards DNA, aiming at anti-cancer and anti-tumor drugs. However, most of these references focused on divalent transition metal cations, such as Co(II), Ni(II), Cu(II) and Zn(II). Only a few efforts have been devoted to high-valent transition metal cations. In this paper, we synthesized two Co(II) and Co(III) complexes, tris{2-(pyridin-2-yl) benzo[d]imidazole-1-ide}-cobalt(III) [denoted as Co(L)3] and bis{2-(pyridin-2-yl)benzo[d]imidazole-1-ide}-{2(pyridin-2-yl)-1H-benzo[d]imidazole}-cobalt(II) [denoted as Co(HL)(L)2], using a diamine ligand 2-(pyridin-2yl)-1H-benzo[d]imidazole (denoted as HL). Their molecular structure was identified with their single crystals, MS and NMR spectra. Co(L)3 and Co(HL)(L)2 share the same crystal system (monoclinic) and space group (P21/ c). Their crystal cell parameters were found rather similar to each other owing to their rather similar ligands, showing a slightly distorted octahedron coordination geometry. No intramolecular interaction, superamolecular interactions and 7C-7C staking between neighboring Co(L)3 molecules or neighboring Co(HL)(L)2 molecules were found, forming a clean coordination environment. Density functional theory calculation on their single crystals suggested that they had similar onset electronic transitions. Their thermal stability, absorption and emission were compared. Owing to their rather similar molecular structures, most of their features were similar. Their binding performance for calf thymus DNA (CT-DNA) was then analyzed and compared. The binding mode between two Co complexes and CT-DNA was analyzed and confirmed by means of viscosity monitoring, circular dichroism analysis, SEM and XRD comparison. Their binding constants were determined as 974.89 M-1 for Co(L)3 and 829.25 M-1 for Co(HL)(L)2, respectively. We found a hydrogen bond between Co(L)3 and CH3OH. No hydrogen bonds were found in Co(HL)(L)2. This result suggested that the Co(L)3 interact with Ct-DNA via the combination of hydrogen bond and weak intermolecular interaction with DNA, while Co(HL)(L)2 interacts with Ct-DNA via only weak intermolecular interaction with DNA. These values were two fold higher than those of literature values (<450 M-1), indicating their potential for DNA treatment.
