期刊：
Journal of Breast Cancer,2019年22(1):15-28 ISSN：1738-6756
通讯作者：
Zu, Xu-Yu
作者机构：
[Shen, Ying-Ying; Peng, Xiu-Da; Zhong, Jing; Zhong, Xiao-Lin; Xiao, Xiao; Ding, Wen-Jun; Chen, Ling; Zu, Xu-Yu; Liu, Jiang-Hua] Univ South China, Affiliated Hosp 1, Inst Clin Med, 69 Chuanshan Rd, Hengyang 421001, Peoples R China.;[Liao, Duan-Fang] Hunan Univ Chinese Med, Key Lab Qual Evaluat Bulk Herbs Hunan Prov, Div Stem Cell Regulat & Applicat, Changsha, Hunan, Peoples R China.;[Xiong, Wei] Cent S Univ, Canc Res Inst, Key Lab Carcinogenesis, Minist Hlth, Changsha, Hunan, Peoples R China.;[Xiong, Wei] Cent S Univ, Canc Res Inst, Key Lab Carcinogenesis & Canc Invas, Minist Educ, Changsha, Hunan, Peoples R China.
通讯机构：
[Zu, Xu-Yu] U;Univ South China, Affiliated Hosp 1, Inst Clin Med, 69 Chuanshan Rd, Hengyang 421001, Peoples R China.
关键词：
Breast neoplasms;Cell proliferation;Pokemon protein, human;SP1;Smad4 protein
摘要：
Purpose: Pokemon, also known as ZBTB7A, belongs to the POZ and Kruppel (POK) family of transcription repressors and is implicated in tumor progression as a key proto-oncogene. This present study aimed at determining the mechanism by which Pokemon inhibits transforming growth factor beta (TGFbeta)-Smad4 pathway-dependent proliferation arrest of breast cancer cells via specificity protein 1 (SP1). Methods: Over-expressing plasmid or small interfering RNA (siRNA) transfection was used to regulate Pokemon levels. The EdU incorporation assay, MTS assay, and clone formation were used to identify the inhibitory effect of Pokemon siRNA on cell proliferation. Quantitative real-time polymerase chain reaction assay confirmed that Pokemon deletion inhibited the expression of proliferation-associated genes. The dual-luciferase reporter assay, electrophoretic mobility shift assay, and co-immunoprecipitation assay were used to analyze binding between Pokemon, Smad4, and SP1. Results: Pokemon deletion induced proliferation arrest of breast cancer cells and inhibited the expression of proliferation-associated genes, especially Smad4. Pokemon bound with SP1 to interdict Smad4 promoter activity. Information on clinical samples was obtained from The Cancer Genome Atlas data, in which the Pokemon mRNA levels showed a negative correlation with Smad4 levels in different subtypes of breast cancer in two independent datasets. Conclusion: We demonstrated that Pokemon binds to SP1 to down-regulate Smad4 expression, thereby promoting proliferation of breast cancer cells. This suggests that Pokemon is a potential TGFbeta-signaling participant in breast cancer progression.
摘要：
Ophiocordyceps xuefengensis has recently been identified as a sister taxon of O. sinensis and has long been recognized as a folk tonic, food, or invigorant in South China. This study had 3 aims: First, we analyzed the antioxidant activities of 3 kinds of caterpillar fungi; the antioxidative ability of the various caterpillar fungi were in the order of O. xuefengensis > Cordyceps militaris > O. sinensis. Second, we further identified the antioxidant compounds in O. xuefengensis by using various methods: multiwavelength DPPH high-performance liquid chromatography with diode array detection and quadrupole time-of-flight mass spectrometry. In this way, we found and identified 30 compounds that possessed potential antioxidant activity. Among these compounds, 23 compounds were determined to be composed of nucleosides, nucleotides, and amino acids. To our knowledge, several compounds in O. xuefengensis are reported here for the first time. Third, we compared the antioxidant markers in 3 caterpillar fungi. Results showed that the caterpillar fungi share some common antioxidants, while each also had its own characteristic compounds that correspond to its antioxidant capacity. This is to our knowledge the first report of the screening and identification of antioxidants in O. xuefengensis, and these results might provide beneficial contributions to understandings of O. xuefengensis on the basis of its chemical compounds and antioxidant activity.
摘要：
Traditional Chinese medicine has great potential to improve wound healing. ANBP, the mixture of 4 Chinese herbs—Agrimoniapilosa, Nelumbonucifera, Boswelliacarteri, and Pollen typhae—is effective in trauma treatment while its mechanism is still elusive. In this study, quantitative proteomics and bioinformatics analyses were performed to decipher the possible roles of ANBP in accelerated wound healing of mouse skin. Among all 3171 identified proteins, 90, 71, 80, and 140 proteins were found to be differently expressed in 6 hours, 3 days, 7 days, and 14 days ANBP-treated tissues compared with corresponding control tissues, respectively. The result showed that different biological processes and pathways were activated at different healing stages. At the early healing stage, ANBP treatment mainly affected several biological processes, including immune and defense response, vascular system restoration, hemostasis and coagulation regulation, lipid metabolism and signal transduction, while muscle tissue, hair, epidermis, extracellular matrix and tissue remodeling related activities were the major events in ANBP promoted later wound healing. This is the first quantitative proteome study of ANBP-treated wound tissues, which provide a new perspective for the mechanism of ANBP accelerated wound healing and is of guiding significance for clinical application of ANBP in trauma disorders cure.
摘要：
Vine tea with strong antioxidant activity is commonly consumed as healthy tea/beverage. However, detailed information about its antioxidants is incomplete. Here, off-line hyphenation of heart-cutting high-speed countercurrent chromatography (HSCCC) with high performance liquid chromatography–diode array detector–quadrupole time-of-flight tandem mass spectrometry (HPLC–DAD–QTOF-MS/MS) were described for systematic profiling antioxidants in vine tea. At first, antioxidants were rapidly screened by 1,1-diphenyl-2-picryl-hydrazyl radical–high performance liquid chromatography (DPPH–HPLC). Subsequently, stepwise HSCCC using petroleum ether–ethyl acetate–methanol–water (4:9:4:9, v/v/v/v) and (4:9:5:8, v/v/v/v) as solvent systems was optimized to fractionate and enrich antioxidants from ethyl acetate fraction of vine tea. Finally, heart-cutting mode was used to collect five interesting HSCCC fractions for HPLC–DAD–QTOF-MS/MS analysis. Desirable orthogonality between HSCCC and HPLC led to identification of fifteen antioxidant flavonoids, while four minor flavonoids were first reported in vine tea. Results showed that the developed system is efficient to comprehensively explore antioxidants from complex natural herbs.
期刊：
Journal of Ethnopharmacology,2017年201:73-81 ISSN：0378-8741
通讯作者：
Liu, Xinmin
作者机构：
[Pan, Ruile; Lu, Cong; Sun, Xiuping; Shi, Zhe; Liu, Xinmin] Chinese Acad Med Sci, Inst Med Plant Dev IMPLAD, Res Ctr Pharmacol & Toxicol, Beijing, Peoples R China.;[Pan, Ruile; Lu, Cong; Sun, Xiuping; Shi, Zhe; Liu, Xinmin] Peking Union Med Coll, Beijing, Peoples R China.;[Chen, Shanguang; Li, Yinghui; Qu, Lina] China Astronaut Res & Training Ctr, Beijing, Peoples R China.;[Sun, Lihua; Dang, Haixia] Chinese Acad Chinese Med Sci, Beijing, Peoples R China.;[Chen, Lingling; Bu, Lanlan; Liu, Xinmin] Hunan Univ Tradit Chinese Med, Changsha, Hunan, Peoples R China.
通讯机构：
[Liu, Xinmin] C;[Liu, Xinmin] P;[Liu, Xinmin] H;Chinese Acad Med Sci, Inst Med Plant Dev IMPLAD, Res Ctr Pharmacol & Toxicol, Beijing, Peoples R China.;Peking Union Med Coll, Beijing, Peoples R China.
期刊：
Analytical and Bioanalytical Chemistry,2016年408(13):3659-3672 ISSN：1618-2642
通讯作者：
Shi, Shu-Yun;Huang, Lu-Qi
作者机构：
[Chen, Wei; Chen, Lin; Cai, Ping; Zhang, Shui-Han] Hunan Acad Chinese Med, Res Inst Chinese Med, Changsha 410013, Hunan, Peoples R China.;[Shi, Shu-Yun; Hu, Xin] Cent South Univ, Coll Chem & Chem Engn, Changsha 410083, Hunan, Peoples R China.;[Huang, Lu-Qi] China Acad Chinese Med Sci, Natl Resource Ctr Chinese Mat Med, Beijing 100700, Peoples R China.
通讯机构：
[Shi, Shu-Yun; Huang, Lu-Qi] C;Cent South Univ, Coll Chem & Chem Engn, Changsha 410083, Hunan, Peoples R China.;China Acad Chinese Med Sci, Natl Resource Ctr Chinese Mat Med, Beijing 100700, Peoples R China.
关键词：
Chlorogenic acid;Diagnostic mass fragmentation analysis;HPLC-DAD-QTOF-MS/MS;Isomer;Lonicera macranthoides;UV spectra screening
摘要：
Lonicerae macranthoides with strong antioxidant activity is commonly used in traditional Chinese medicine and folk tea/beverage. However, detailed information about its antioxidant activity and bioactive compounds is limited. Then at first, we comparatively evaluated total phenolic content (TPC), total flavonoid content (TFC) and antioxidant activities of water extract, petroleum ether, ethyl acetate and n-butanol fractions of L. macranthoides. Ethyl acetate fraction exhibited the highest level of TPC (207.38 mg GAE/g DW), TFC (53.06 mg RE/g DW) and the best DPPH scavenge activity and reducing power. n-Butanol fraction showed the best ABTS+ and O2− scavenging activities. Interestingly, water extract, ethyl acetate and n-butanol fractions showed stronger antioxidant activities than positive control, butylated hydroxytoluene (BHT). After that, thirty-one antioxidant phenolic compounds, including twenty-two phenolic acids and nine flavonoids, were screened by DPPH–HPLC experiment and then identified using HPLC–DAD–QTOF-MS/MS. It is noted that twenty-one compounds (1, 3–4, 6–17, 19, 23, 26, 28–29, and 31), as far as was known, were discovered from L. macranthoide for the first time, and eleven of them (3–4, 10–17, and 23) were reported in Lonicera species for the first time. Results indicated that L. macranthoides could serve as promising source of rich antioxidants in foods, beverages and medicines for health promotion.
摘要：
The present study was to determine the targeting effect of M13 phage peptide ZL4 (MppZL4) on Schistosoma japonicum (S.j). Mice infected with S.j were injected with MppZL4. Real-time PCR was used to detect the distribution and metabolism of MppZL4 in the livers and lungs of mice. In vivo refusion test was performed to detect the targeting of MppZL4. Western blotting was employed to determine the expression of MppZL4. Live imaging was used to detect the distribution of oligopeptide MppZL4. Immunohistochemistry was employed to determine MppZL4 location on adult S.j body surface. Gomori method was employed to detect the influence of oligopeptide MppZL4 on alkaline phosphatase activity. The distribution and metabolism of MppZL4 and M13KE are not significantly different from each other at each time point. The abundance of MppZL4 is changed as S.j migrates in mice. The targeted binding effect of MppZL4 varies at different stages. ZL4 oligopeptide targets S.j in mice. The specific binding sites of MppZL4 on S.j body are mainly located in syncytial cells. The binding sites of MppZL4 on S.j body surface might be ALP or ALP-related proteins. MppZL4 had targeted binding effect on S.j with its binding site being associated with proteins related to S.j alkaline phosphatase. S.j tegument had a specifically binding site with exogenous peptides, offering new means to explore the interactions between hosts and parasites. Additionally, MppZL4 can possibly be used as targeting molecules in worm-resistant drugs or as tracing molecules in imaging diagnosis technologies.
期刊：
International Journal of Lower Extremity wounds,2015年14(4):335-342 ISSN：1534-7346
通讯作者：
Fu, Xiao-Bing
作者机构：
[Dong, Liang; Liu, Jie-Jie; Han, Wei-Dong; Hao, Hao-Jie; Tong, Chuan; Li, Mei-Rong; Fu, Xiao-Bing; He, Wen-Jun; Chen, Li; Hou, Qian] Chinese Peoples Liberat Army Gen Hosp, Beijing 100048, Peoples R China.;[Hou, Qian] Chinese PLA 148th Hosp, Zibo, Peoples R China.;[Fu, Xiao-Bing; He, Wen-Jun] Chinese Peoples Liberat Army Gen Hosp, Affiliated Hosp 1, Beijing 100048, Peoples R China.;[Chen, Li] Hunan Univ Chinese Med, Changsha, Hunan, Peoples R China.;[Zhou, Zhong-Zhi] Hunan Univ Chinese Med, Affiliated Hosp 1, Changsha, Hunan, Peoples R China.
通讯机构：
[Fu, Xiao-Bing] C;Chinese Peoples Liberat Army Gen Hosp, Affiliated Hosp 1, Wound Healing & Cell Biol Lab, 51 Fucheng Rd, Beijing 100048, Peoples R China.
关键词：
wound healing;herbal medicine;collagen;diabetic mouse model
摘要：
Wound healing is a troublesome problem in diabetic patients. Besides, there is also an increased risk of postsurgical wound complications for diabetic patient. It has been revealed that traditional Chinese medicine may promote healing and inhibit scar formation, while the changes of morphology and physiology of wounds on such medicine treatment still remain elusive. In this study, we first used the ultralow temperature preparation method to produce mixed superfine powder from Agrimonia pilosa (A), Nelumbo nucifera (N), Boswellia carteri (B), and Pollen typhae (P), named as ANBP. Applying ANBP on 40 streptozotocin (STZ)-induced diabetic C57BL/6 mice (4-6 weeks, 20 ± 2 g), we observed that the wound healing process was accelerated and the wound healing time was shortened (14 days, P < .05). Pathological observation using hematoxylin–eosin staining indicated that inflammatory cells were reduced (P < .05) while the thickness of granulation tissue and length of epithelial tongue were increased (P < .05). The vascular density was increased on 7 and 14 days after ANBP treatment. Masson and Sirius red staining showed that, at the early stage of trauma, the expressions of Col I and Col III, especially Col III, were increased in the ANBP group (P < .05). Studies in vitro demonstrated that tubular formation was significantly increased after ANBP treatment on human vascular endothelial cells in a dose-dependent way. Taken together, our studies revealed that ANBP treatment could accelerate wound healing, promote vascularization, and inhibit inflammation, suggesting the potential clinic application of ANBP for diabetes mellitus and refractory wounds.
作者机构：
[Zhai, Jingming; Wang, Wei; Zhang, Xiaoru; Fan, Yonggang] Departments of General Surgery, the First Affiliated Hospital, Henan University of Science and Technology, Luoyang, Henan 471000, China;[Wang, Wei; Chen, Lili] These authors contributed equally to this work;[Chen, Lili] Department of Pathology, the First Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong 510080, China;[Tang, Qun] Department of Pathology, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China
通讯机构：
Departments of General Surgery, First Affiliated Hospital, Henan University of Science and Technology, 24 Jinghua Road, Luoyang, Henan, China
摘要：
AIM OF THE STUDY: The Ras-related tumour suppressor gene aplasia Ras homolog member I (ARHI) is downregulated in many types of cancer, including ovarian cancer and hepatocellular carcinoma. In the present study, we explore the expression level and role of ARHI in colon cancer. Moreover, the mechanisms that down-regulate expression of ARHI in colon cancer will be further investigated. MATERIAL AND METHODS: ARHI expression levels were evaluated with immunohistochemistry, reverse transcriptase-PCR, and western blot. Loss of heterozygosity (LOH), single strand conformation polymorphism (SSCP), and methylation-specific PCR (MSP) were used to study the mechanisms of ARHI down-regulation. RESULTS: Low expression of ARHI was observed in 61.7% (37/60) of colon cancer specimens. Compared with the paired noncancerous tissues, ARHI expression was significantly decreased in colon cancer tissues. Furthermore, low ARHI expression was significantly associated with worse differentiation degree and Dukes' stage (P < 0.05). Methylation-specific PCR assay revealed that the methylation rates of ARHI were 53.3% (16/30) and 46.7% (14/30) in ARHI CpG I and CpG II, respectively. Therefore, methylation of promoter may be involving in down regulation of ARHI expression. CONCLUSIONS: These data highlight an important role for ARHI in colon cancer, which could be a therapeutic strategy against this malignancy.
