作者： Jin, Jian;Liu, Hao;Zhong, Can;Xie, Jing;Qin, You;...

期刊： Mitochondrial DNA Part B-Resources,2020年5(1):1111-1112 ISSN：2380-2359

通讯作者： Cai, Ping;Zhang, SH

作者机构： [Liu, Hao; Zhang, Shuihan; Xie, Jing; Jin, Jian; Liang, Xuejuan; Chen, Lin; Cai, Ping; Zhang, SH; Cai, P; Qin, You; Zhong, Can] Hunan Acad Chinese Med, Inst Chinese Mat Med, Changsha, Peoples R China.;[Jin, Jian] Hunan Univ Chinese Med, Grad Sch, Changsha, Peoples R China.

通讯机构： [Zhang, SH ; Cai, P] H;Hunan Acad Chinese Med, Inst Chinese Mat Med, Changsha, Peoples R China.

关键词： Lilium pumilum;High-throughput sequencing;Chloroplast;Genome sequence

摘要： Lilium pumilum DC. is a useful plant species not only for its showy flowers but also for its edible and medicinal values. Here we report on the complete chloroplast genome sequence of L. pumilum. The chloroplast genome is 152,573 bp in size and includes two inverted repeat regions of 52,984 bp, which is separated by a large single-copy region of 82,009 bp and a small single copy region of 17,580 bp. A total of 130 genes were predicted, including 38 tRNA, 8 rRNA, and 84 protein-coding genes. Phylogenetic analysis placed L. pumilum under the family Liliaceae. © 2020, © 2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

语种： 英文

作者： Gao, Q.;Lu, F.;Chen, L.;Ling, Y.;Wei, K.;...

作者机构： [Lu, F.; Wei, K.; Ling, Y.; Chen, L.; Gao, Q.; Hu, J.] Hunan Univ Chinese Med, Changsha, Hunan, Peoples R China.

会议名称： 17th International Congress of Immunology (IUIS)

会议时间： OCT 19-23, 2019

会议地点： Beijing, PEOPLES R CHINA

关键词： Prescription of Chan-Shan-Hu-Ji;Proliferation;Inhibition;Cell apoptosis

语种： 英文

作者： Chen, Ling;Zhong, Jing;Liu, Jiang-Hua;Liao, Duan-Fang;Shen, Ying-Ying;...

期刊： JOURNAL OF BREAST CANCER,2019年22(1):15-28 ISSN：1738-6756

通讯作者： Zu, XY

作者机构： [Shen, Ying-Ying; Peng, Xiu-Da; Zhong, Jing; Zhong, Xiao-Lin; Zu, Xu-Yu; Xiao, Xiao; Ding, Wen-Jun; Chen, Ling; Liu, Jiang-Hua] Univ South China, Affiliated Hosp 1, Inst Clin Med, 69 Chuanshan Rd, Hengyang 421001, Peoples R China.;[Liao, Duan-Fang] Hunan Univ Chinese Med, Key Lab Qual Evaluat Bulk Herbs Hunan Prov, Div Stem Cell Regulat & Applicat, Changsha, Hunan, Peoples R China.;[Xiong, Wei] Cent S Univ, Canc Res Inst, Key Lab Carcinogenesis, Minist Hlth, Changsha, Hunan, Peoples R China.;[Xiong, Wei] Cent S Univ, Canc Res Inst, Key Lab Carcinogenesis & Canc Invas, Minist Educ, Changsha, Hunan, Peoples R China.

通讯机构： [Zu, XY ] U;Univ South China, Affiliated Hosp 1, Inst Clin Med, 69 Chuanshan Rd, Hengyang 421001, Peoples R China.

关键词： kruppel like factor;pokemon;Smad4 protein;small interfering RNA;transcription factor Sp1;transforming growth factor beta;unclassified drug;Article;breast cancer;cancer cell line;cell cycle arrest;cell proliferation;cell viability;cloning;colony formation;down regulation;fluorescence microscopy;gel mobility shift assay;gene deletion;gene expression regulation;gene overexpression;human;human cell;immunoprecipitation;luciferase assay;microarray analysis;MTS assay;polyacrylamide gel electrophoresis;promoter region;protein expression;real time polymerase chain reaction;signal transduction;Western blotting

摘要： Purpose: Pokemon, also known as ZBTB7A, belongs to the POZ and Krüppel (POK) family of transcription repressors and is implicated in tumor progression as a key proto-oncogene. This present study aimed at determining the mechanism by which Pokemon inhibits transforming growth factor β (TGFβ)-Smad4 pathway-dependent proliferation arrest of breast cancer cells via specificity protein 1 (SP1). Methods: Over-expressing plasmid or small interfering RNA (siRNA) transfection was used to regulate Pokemon levels. The EdU incorporation assay, MTS assay, and clone formation were used to identify the inhibitory effect of Pokemon siRNA on cell proliferation. Quantitative real-time polymerase chain reaction assay confirmed that Pokemon deletion inhibited the expression of proliferation-associated genes. The dual-luciferase reporter assay, electrophoretic mobility shift assay, and co-immunoprecipitation assay were used to analyze binding between Pokemon, Smad4, and SP1. Results: Pokemon deletion induced proliferation arrest of breast cancer cells and inhibited the expression of proliferation-associated genes, especially Smad4. Pokemon bound with SP1 to interdict Smad4 promoter activity. Information on clinical samples was obtained from The Cancer Genome Atlas data, in which the Pokemon mRNA levels showed a negative correlation with Smad4 levels in different subtypes of breast cancer in two independent datasets. Conclusion: We demonstrated that Pokemon binds to SP1 to down-regulate Smad4 expression, thereby promoting proliferation of breast cancer cells. This suggests that Pokemon is a potential TGFβ-signaling participant in breast cancer progression. © 2019 Korean Breast Cancer Society.

语种： 英文

作者： Zhou, Zhengbing;Long, Da;Hsu, Chih-Chien;Liu, Huanhuan;Chen, Long;...

期刊： Acta Biomaterialia,2019年97:310-320 ISSN：1742-7061

通讯作者： Tuffaha, S;Mao, HQ

作者机构： [Tang, Juyu; Zhou, Zhengbing] Cent South Univ, Xiangya Hosp, Dept Hand & Microsurg, Changsha 410008, Hunan, Peoples R China.;[Li, Xiaowei; Liu, Huanhuan; Zhou, Zhengbing; Mao, Hai-Quan; Chen, Long] Johns Hopkins Univ, Sch Med, Translat Tissue Engn Ctr, Baltimore, MD 21287 USA.;[Li, Xiaowei; Zhou, Zhengbing; Mao, Hai-Quan; Chen, Long] Johns Hopkins Univ, Inst NanoBioTechnol, Baltimore, MD 21218 USA.;[Long, Da] Hunan Univ Chinese Tradit Med, Hosp 1, Dept Ophthalmol, Changsha 410007, Hunan, Peoples R China.;[Hsu, Chih-Chien; Long, Da; Lin, Hui; Yiu, Samuel] Johns Hopkins Univ, Sch Med, Wilmer Eye Inst, Baltimore, MD 21287 USA.

通讯机构： [Mao, HQ ] 3;[Tuffaha, S ] 7;720 Rutland Ave,Russ 749D, Baltimore, MD 21205 USA.;3400 N Charles St,Croft Hall 100, Baltimore, MD 21218 USA.

关键词： Amniotic membrane;Composite membrane;Electrospinning;Limbal stem cell deficiency;Nanofibers;Transplantation

摘要： Human amniotic membrane (AM) offers unique advantages as a matrix to support the transplantation of limbal stem cells (LSCs) due to its inherent pro-regenerative and anti-inflammatory properties. However, the widespread use of AM in clinical treatments of ocular surface disorders is limited by its weak mechanical strength and fast degradation, and high cost associated with preserving freshly isolated AM. Here we constructed a composite membrane consisting of an electrospun bioabsorbable poly(epsilon-caprolactone) (PCL) nanofiber mesh to significantly improve the ultimate tensile strength, toughness, and suture retention strength by 4-10-fold in comparison with decellularized AM sheet. The composite membrane showed extended stability and conferred longer-lasting coverage on wounded cornea surface compared with dAM. The composite membrane maintained the pro-regenerative and immunomodulatory properties of dAM, promoted LSC survival, retention, and organization, improved re-epithelialization of the defect area, and reduced inflammation and neovascularization. This study demonstrates the translational potential of our composite membrane for stem cell-based treatment of ocular surface damage. Statement of Significance Human decellularized amniotic membrane (dAM) has been widely shown as a biodegradable and bioactive matrix for regenerative tissue repair. However, the weak mechanical property has limited its widespread use in the clinic. Here we constructed a composite membrane using a layer of electrospun poly(e-caprolactone) (PCL) nanofiber mesh to reinforce the dAM sheet through covalent interfacial bonding, while retaining the unique bioactivity of dAM. In a rabbit model of limbal stem cell (LSC) deficiency induced by alkaline burn, we demonstrated the superior property of this PCL-dAM composite membrane for repairing damaged cornea through promoting LSC transplantation, improving re-epithelialization, and reducing inflammation and neovascularization. This new composite membrane offers great translational potential in supporting stem cell-based treatment of ocular surface damage. (C) 2019 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.

语种： 英文

作者： Jin, Jian;Kang, Wenli;Zhong, Can;Qin, You;Zhou, Rongrong;...

期刊： Journal of Ethnopharmacology,2018年219:195-201 ISSN：0378-8741

通讯作者： Qin, Yuhui;Zhang, SH

作者机构： [Liu, Hao; Zhang, Shuihan; Kang, Wenli; Qin, Yuhui; Xie, Jing; Jin, Jian; Chen, Lin; Zhang, SH; Qin, You; Zhong, Can] Hunan Acad Chinese Med, Inst Chinese Mat Med, Changsha 410013, Hunan, Peoples R China.;[Jin, Jian; Qin, You; Qin, Yuhui] Hunan Univ Chinese Med, Changsha 410208, Hunan, Peoples R China.;[Zhong, Can] Hunan Agr Univ, Hort & Landscape Coll, Changsha 410128, Hunan, Peoples R China.;[Zhou, Rongrong] Changchun Univ Chinese Med, Changchun 130117, Jilin, Peoples R China.;[Liu, Hao; Zhang, Shuihan; Xie, Jing; Chen, Lin] 2011 Collaborat & Innovat Ctr Digital Chinese Med, Changsha 410208, Hunan, Peoples R China.

通讯机构： [Qin, YH; Zhang, SH ] H;Hunan Acad Chinese Med, Inst Chinese Mat Med, Changsha 410013, Hunan, Peoples R China.

关键词： antineoplastic agent;cisplatin;cordycepin;Ophiocordyceps xuefengensis extract;plant extract;transcriptome;unclassified drug;antineoplastic agent;A-549 cell line;antineoplastic activity;Article;biosynthesis;controlled study;Cordyceps;DNA library;Eca-109 cell line;gene expression;gene ontology;HeLa cell line;Hep-G2 cell line;high performance liquid chromatography;HT-29 cell line;human;human cell;in vitro study;MCF-7 cell line;nonhuman;Ophiocordyceps;Ophiocordyceps xuefengensis;Raji cell line;RNA isolation;transcriptomics;animal;cell proliferation;Chinese medicine;drug effect;gene expression profiling;genetics;isolation and purification;physiology;procedures;trends;A549 Cells;Animals;Antineoplastic Agents;Cell Proliferation;Cordyceps;Gene Expression Profiling;HeLa Cells;Hep G2 Cells;HT29 Cells;Humans;MCF-7 Cells;Medicine, Chinese Traditional

摘要： Ethnopharmacological relevance: The Yao ethnic group in Xuefeng Mountains area have used Xuefeng cordyceps, the caterpillar–fungus complex of Ophiocordyceps xuefengensis, for treating a variety of diseases for long. Just like some other cordyceps, O. xuefengensis, which is identified as the sister taxon of O. sinensis in 2013, also seems to have broad pharmacological properties, not only enhancing human immunity, anti–bacteria, anti–virus, but also anti–tumor. However, investigation of the medicinal fugal species O. xuefengensis can be found only in few literature records since its pharmacological and therapeutic use is mainly in traditional Yao communities by local healers. Aim of the study: The aim of this study is to collect samples of Xuefeng cordyceps and isolate the strain of O. xuefengensis, to determine bioactive components and evaluate the anti-tumor activity, to obtain the gene expression profile of O. xuefengensis and reveal its pharmacological properties by de novo transcriptome analysis. Accordingly, we attempt to provide information and give a comprehensive understanding of this mysterious medicinal fugal species from traditional Yao communities of China. Material and methods: Bioactive components were determined with HPLC–DAD–Q–TOF–MS technology; in vitro anti–tumor activity against 6 cell lines was evaluated using standard MTT assay; transcriptome analysis was done by de novo sequencing; unique genes were functionally profiled basing on Gene Ontology Database and the targeted genes were examined by blast. Results: Trace cordycepin, an anti–tumor agent, was detected in O. xuefengensis water extract. To some extent, the raw water extract of O. xuefengensis showed in vitro anti–tumor activity, against A549, HepG2, MCF–7, PC–3 and Raji cell lines. A total of 94,858 transcripts and 49,001 unique genes were obtained, amongst, 43.4% unique genes were matched with those of O. sinensis. Not all supposed genes related to cordycepin biosynthetic pathways were found by transcriptome analysis. Conclusion: According to the gene expression profile, O. xuefengensis is very close to medicinal fungus O. sinensis. Raw water extract of O. xuefengensis, to a certain degree, could inhibit the growth of tumor cells, indicating that this fungus could be a new resource for the exploration of anti–tumor drug. © 2018 Elsevier B.V.

语种： 英文

作者： Qin, You;Chen, Lin;Jin, Jian;Huang, Jianhua;Li, Jiayu;...

期刊： INTERNATIONAL JOURNAL OF MEDICINAL MUSHROOMS,2018年20(9):887-899 ISSN：1521-9437

通讯作者： Zhang, SH

作者机构： [Li, Jiayu; Zhou, Qingyijun; Jin, Jian; Huang, Jianhua; Qin, You] Hunan Univ Chinese Med, Changsha, Hunan, Peoples R China.;[Li, Jiayu; Zhang, Shuihan; Zhou, Qingyijun; Jin, Jian; Chen, Lin; Zhang, SH; Qin, You] Hunan Acad Chinese Med, Inst Chinese Mat Med, 8 Yuehua Rd, Changsha 410013, Hunan, Peoples R China.;[Zhang, Shuihan; Chen, Lin] 2011 Collaborat & Innovat Ctr Digital Chinese Med, Changsha, Hunan, Peoples R China.;[Zhou, Rongrong] Changchun Univ Chinese Med, Changchun, Jilin, Peoples R China.

通讯机构： [Zhang, SH ] H;Hunan Acad Chinese Med, Inst Chinese Mat Med, 8 Yuehua Rd, Changsha 410013, Hunan, Peoples R China.

关键词： 2 3 cyclic AMP;2 deoxy 3 AMP;3 adenylic acid;3 guanylic acid;3,5 dimothoxy tyrosine;adenine;adenosine;adenosine phosphate;antioxidant;ascorbic acid;cordycepin;fungal extract;glycylleucine;guanosine;guanosine phosphate;hypoxanthine;leucine;methionine;n,n (1,9 dioxo 1,9 nonanediyl)bis glycine;n6 succinyl adenosine;phenylalanine;picolinic acid;pipemidic acid;pyroglutamic acid;tryptophan;tyrosine;unclassified drug;uridine;valine;1,1-diphenyl-2-picrylhydrazyl;antioxidant;biphenyl derivative;picric acid;antioxidant assay;Article;comparative study;Cordyceps;DPPH radical scavenging assay;drug identification;drug screening;electrospray;fungal cell culture;high performance liquid chromatography;nonhuman;Ophiocordyceps;Ophiocordyceps xuefengensis;quadrupole mass spectrometry;qualitative analysis;retention time;temperature;time of flight mass spectrometry;Ascomycetes;chemistry;fruiting body;procedures;tandem mass spectrometry;Antioxidants;Ascomycota;Biphenyl Compounds;Chromatography, High Pressure Liquid;Fruiting Bodies, Fungal;Picrates;Tandem Mass Spectrometry

摘要： Ophiocordyceps xuefengensis has recently been identified as a sister taxon of O. sinensis and has long been recognized as a folk tonic, food, or invigorant in South China. This study had 3 aims: First, we analyzed the antioxidant activities of 3 kinds of caterpillar fungi; the antioxidative ability of the various caterpillar fungi were in the order of O. xuefengensis > Cordyceps militaris > O. sinensis. Second, we further identified the antioxidant compounds in O. xuefengensis by using various methods: multiwavelength DPPH high-performance liquid chromatography with diode array detection and quadrupole time-of-flight mass spectrometry. In this way, we found and identified 30 compounds that possessed potential antioxidant activity. Among these compounds, 23 compounds were determined to be composed of nucleosides, nucleotides, and amino acids. To our knowledge, several compounds in O. xuefengensis are reported here for the first time. Third, we compared the antioxidant markers in 3 caterpillar fungi. Results showed that the caterpillar fungi share some common antioxidants, while each also had its own characteristic compounds that correspond to its antioxidant capacity. This is to our knowledge the first report of the screening and identification of antioxidants in O. xuefengensis, and these results might provide beneficial contributions to understandings of O. xuefengensis on the basis of its chemical compounds and antioxidant activity. © 2018 Begell House, Inc.

语种： 英文

作者： Liu, Ze;Xie, Hua-lin;Chen, Lin;Huang, Jian-hua

期刊： Molecules,2018年23(5):1058 ISSN：1420-3049

通讯作者： Huang, JH

作者机构： [Huang, Jian-hua; Liu, Ze] Hunan Acad Chinese Med, Changsha 410013, Hunan, Peoples R China.;[Chen, Lin; Huang, Jian-hua; Liu, Ze] Hunan Univ Chinese Med, Sch Pharm, Changsha 410208, Hunan, Peoples R China.;[Xie, Hua-lin] Yangtze Normal Univ, Sch Chem & Engn, Chongqing 408100, Peoples R China.

通讯机构： [Huang, JH ] H;Hunan Acad Chinese Med, Changsha 410013, Hunan, Peoples R China.;Hunan Univ Chinese Med, Sch Pharm, Changsha 410208, Hunan, Peoples R China.

关键词： antioxidant;polyphenol;polysaccharide;chemistry;infrared spectrophotometry;procedures;tea;Antioxidants;Polyphenols;Polysaccharides;Spectrophotometry, Infrared;Tea

摘要： Background: Pu-erh tea is a unique microbially fermented tea, which distinctive chemical constituents and activities are worthy of systematic study. Near infrared spectroscopy (NIR) coupled with suitable chemometrics approaches can rapidly and accurately quantitatively analyze multiple compounds in samples. Methods: In this study, an improved weighted partial least squares (PLS) algorithm combined with near infrared spectroscopy (NIR) was used to construct a fast calibration model for determining four main components, i.e., tea polyphenols, tea polysaccharide, total flavonoids, theanine content, and further determine the total antioxidant capacity of pu-erh tea. Results: The final correlation coefficients R square for tea polyphenols, tea polysaccharide, total flavonoids content, theanine content, and total antioxidant capacity were 0.8288, 0.8403, 0.8415, 0.8537 and 0.8682, respectively. Conclusions: The current study provided a comprehensive study of four main ingredients and activity of pu-erh tea, and demonstrated that NIR spectroscopy technology coupled with multivariate calibration analysis could be successfully applied to pu-erh tea quality assessment. © 2018 by the authors.

语种： 英文

作者： Jin, Jian;Zhong, Can;Qin, You;Cai, Yuan;Zhen, Lanping;...

期刊： FEMS Microbiology Letters,2017年364(20):- ISSN：0378-1097

通讯作者： Zhang, SH

作者机构： [Zhang, Shuihan; Jin, Jian; Cai, Yuan; Chen, Lin; Zhang, SH; Shen, Bingbing; Wan, Dan; Qin, You; Zhong, Can; Zhen, Lanping; Qin, Yuhui] Hunan Acad Chinese Med, Inst Chinese Mat Med, 8 Rue Yuehua, Changsha 410013, Hunan, Peoples R China.;[Jin, Jian; Qin, Yuhui] Hunan Univ Chinese Med, Changsha 410208, Hunan, Peoples R China.;[Zhong, Can] Hunan Agr Univ, Hort & Landscape Coll, Changsha 410128, Hunan, Peoples R China.;[Zhang, Shuihan; Cai, Yuan; Chen, Lin; Shen, Bingbing; Wan, Dan; Zhen, Lanping] 2011 Collaborat & Innovat Ctr Digital Chinese Med, Changsha 410208, Hunan, Peoples R China.

通讯机构： [Zhang, SH ] H;Hunan Acad Chinese Med, Inst Chinese Mat Med, 8 Rue Yuehua, Changsha 410013, Hunan, Peoples R China.

关键词： adenosine;artificial infection;caterpillar fungi;cordycepin;HPLC-DAD-Q-TOF-MS/MS;Ophiocordyceps xuefengensis

摘要： Caterpillar fungi have numerous pharmacological and therapeutic applications in traditional medicine, due to a variety of active chemical constituents, such as cordycepin and adenosine. It is imperative to discover new resource for artificial cultivation and biometabolite production since the traditional natural species are endangered. In this study, a new strain HACM 001 was isolated and identified as Ophiocordyceps xuefengensis by rDNA-ITS sequencing. This strain showed the potential of artificial infection to caterpillar larvae leading to mummification, as well as fermentation mycelia in liquid culture and cultivation stromata in solid medium. Eight nucleosides and nucleobases, especially cordycepin and adenosine, were determined and analyzed with HPLC-DAD-Q-TOF-MS/MS technology. Cordycepin was detected in all forms of present O. xuefengensis strain at different contents, among which the highest content (37.1 μg/g) appeared in the stromata cultivated on solid medium. The content of adenosine in mycelia and stromata, respectively, reached 1155 μg/g and 1470 μg/g. Therefore, O. xuefengensis might be an alternative source for obtaining artificial fungus-caterpillar-larvae complex and producing cordycepin and adenosine. © 2017 FEMS. All rights reserved.

语种： 英文

作者： Zeng, Kui;Chen, Lang*;Chen, Yi（陈懿）;Liu, Yongping（刘永平）;Zhou, Yongbo;...

期刊： ADVANCED SYNTHESIS & CATALYSIS,2017年359(5):841-847 ISSN：1615-4150

通讯作者： Chen, Lang;Yin, SF

作者机构： [Zhou, Yongbo; Chen, Lang; Yin, Shuang-Feng; Zeng, Kui; Yin, SF] Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Hunan, Peoples R China.;[Liu, Yongping; Chen, Yi] Hunan Univ Chinese Med, Coll Med, Changsha 410208, Hunan, Peoples R China.;[Au, Chak-Tong] Hunan Inst Engn, Coll Chem & Chem Engn, Xiangtan 411104, Hunan, Peoples R China.

通讯机构： [Chen, L; Yin, SF ] H;Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Hunan, Peoples R China.

关键词： alkenes;alkynes;β-chloroalkyl sulfones;β-haloalkenyl sulfones;sodium arenesulfinates

摘要： <jats:title>Abstract</jats:title><jats:p>The atom‐economic and one‐pot regio‐ and stereoselective addition of sodium arenesulfinates to either alkynes or alkenes can be achieved with an iron(III) chloride hexahydrate [FeCl<jats:sub>3</jats:sub>⋅6 H<jats:sub>2</jats:sub>O] catalytic system to afford β‐haloalkenyl and β‐chloroalkyl sulfones in moderate to good yields.</jats:p><jats:p><jats:boxed-text content-type="graphic" position="anchor"><jats:graphic xmlns:xlink="http://www.w3.org/1999/xlink" mimetype="image/png" position="anchor" specific-use="enlarged-web-image" xlink:href="graphic/adsc201601211-toc-0001-m.png"><jats:alt-text>magnified image</jats:alt-text></jats:graphic></jats:boxed-text> </jats:p>

语种： 英文

作者： Chen, Li;Hou, Qian;Zhou, Zhong-Zhi;Li, Mei-Rong;Zhong, Ling-Zhi;...

期刊： INTERNATIONAL JOURNAL OF LOWER EXTREMITY WOUNDS,2017年16(3):154-162 ISSN：1534-7346

通讯作者： He, Wen-Jun;Fu, XB

作者机构： [Zhong, Ling-Zhi; He, Wen-Jun; Fu, XB; Cheng, Zhong-Yi; Fu, Xiao-Bing; Chen, Li; Zhu, Zi-Ying; Li, Mei-Rong; Deng, Xiang-Dong; Hou, Qian] Chinese Peoples Liberat Army Gen Hosp, Inst Basic Med, 28 Fuxing Rd, Beijing 100853, Peoples R China.;[Chen, Li] Hunan Univ Chinese Med, Changsha, Hunan, Peoples R China.;[Zhou, Zhong-Zhi; Xiang, Cong-Lian] Hunan Univ Chinese Med, Affiliated Hosp 1, Changsha, Hunan, Peoples R China.;[Cheng, Zhong-Yi; Zhu, Jun] Jingjie PTM Biolab Hangzhou Co Ltd, Hangzhou, Zhejiang, Peoples R China.;[He, Wen-Jun; Fu, XB; Fu, Xiao-Bing] Chinese Peoples Liberat Army Gen Hosp, Affiliated Hosp 1, 51 Fucheng Rd, Beijing 100048, Peoples R China.

通讯机构： [He, WJ; Fu, XB ] C;Chinese Peoples Liberat Army Gen Hosp, Inst Basic Med, 28 Fuxing Rd, Beijing 100853, Peoples R China.;Chinese Peoples Liberat Army Gen Hosp, Affiliated Hosp 1, 51 Fucheng Rd, Beijing 100048, Peoples R China.

关键词： ANBP;Chinese drug;unclassified drug;wound healing promoting agent;herbaceous agent;Agrimonia pilosa;animal experiment;animal model;animal tissue;Article;Boswellia sacra;Chinese medicine;controlled study;down regulation;extracellular matrix;gene ontology;granulation tissue;iTRAQ labeling;liquid chromatography-mass spectrometry;male;medicinal plant;mouse;Nelumbo nucifera;nonhuman;Pollen typhae;priority journal;protein analysis;protein expression;proteomics;punch biopsy;quantitative analysis;skin injury;thickness;upregulation;wound closure;wound healing;animal;C57BL mouse;comparative study;disease model;drug effect;genetics;immunohistochemistry;injury;needle biopsy;pathology;randomization;reference value;sensitivity and specificity;skin;wound healing;Animals;Biopsy, Needle;Disease Models, Animal;Drugs, Chinese Herbal;Immunohistochemistry;Male;Mice;Mice, Inbred C57BL;Proteomics;Random Allocation;Reference Values;Sensitivity and Specificity;Skin;Wound Healing;Wounds and Injuries

摘要： Traditional Chinese medicine has great potential to improve wound healing. ANBP, the mixture of 4 Chinese herbs—Agrimoniapilosa, Nelumbonucifera, Boswelliacarteri, and Pollen typhae—is effective in trauma treatment while its mechanism is still elusive. In this study, quantitative proteomics and bioinformatics analyses were performed to decipher the possible roles of ANBP in accelerated wound healing of mouse skin. Among all 3171 identified proteins, 90, 71, 80, and 140 proteins were found to be differently expressed in 6 hours, 3 days, 7 days, and 14 days ANBP-treated tissues compared with corresponding control tissues, respectively. The result showed that different biological processes and pathways were activated at different healing stages. At the early healing stage, ANBP treatment mainly affected several biological processes, including immune and defense response, vascular system restoration, hemostasis and coagulation regulation, lipid metabolism and signal transduction, while muscle tissue, hair, epidermis, extracellular matrix and tissue remodeling related activities were the major events in ANBP promoted later wound healing. This is the first quantitative proteome study of ANBP-treated wound tissues, which provide a new perspective for the mechanism of ANBP accelerated wound healing and is of guiding significance for clinical application of ANBP in trauma disorders cure.

语种： 英文

作者： Gao, Qingping;Ma, Ruyi;Chen, Lin;Shi, Shuyun;Cai, Ping;...

期刊： Food Chemistry,2017年225:55-61 ISSN：0308-8146

通讯作者： Shi, SY;Xiang, HY

作者机构： [Gao, Qingping] Cent South Univ, Dept Orthodont, Xiangya Hosp, Changsha 410078, Hunan, Peoples R China.;[Shi, SY; Shi, Shuyun; Gao, Qingping; Ma, Ruyi] Cent South Univ, Coll Chem & Chem Engn, Changsha 410083, Hunan, Peoples R China.;[Zhang, Shuihan; Chen, Lin; Cai, Ping] Hunan Acad Chinese Med, Res Inst Chinese Med, Changsha 410013, Hunan, Peoples R China.;[Xiang, Haiyan; Xiang, HY] Southern Med Univ, Sch Pharmaceut Sci, Guangzhou 510515, Guangdong, Peoples R China.

通讯机构： [Shi, SY ] C;[Xiang, HY ] S;Cent South Univ, Coll Chem & Chem Engn, Changsha 410083, Hunan, Peoples R China.;Southern Med Univ, Sch Pharmaceut Sci, Guangzhou 510515, Guangdong, Peoples R China.

关键词： Antioxidant activity;Flavonoid;HPLC-DAD-QTOF-MS/MS;HSCCC;Vine tea

摘要： Vine tea with strong antioxidant activity is commonly consumed as healthy tea/beverage. However, detailed information about its antioxidants is incomplete. Here, off-line hyphenation of heart-cutting high-speed countercurrent chromatography (HSCCC) with high performance liquid chromatography–diode array detector–quadrupole time-of-flight tandem mass spectrometry (HPLC–DAD–QTOF-MS/MS) were described for systematic profiling antioxidants in vine tea. At first, antioxidants were rapidly screened by 1,1-diphenyl-2-picryl-hydrazyl radical–high performance liquid chromatography (DPPH–HPLC). Subsequently, stepwise HSCCC using petroleum ether–ethyl acetate–methanol–water (4:9:4:9, v/v/v/v) and (4:9:5:8, v/v/v/v) as solvent systems was optimized to fractionate and enrich antioxidants from ethyl acetate fraction of vine tea. Finally, heart-cutting mode was used to collect five interesting HSCCC fractions for HPLC–DAD–QTOF-MS/MS analysis. Desirable orthogonality between HSCCC and HPLC led to identification of fifteen antioxidant flavonoids, while four minor flavonoids were first reported in vine tea. Results showed that the developed system is efficient to comprehensively explore antioxidants from complex natural herbs. © 2016 Elsevier Ltd

语种： 英文

作者： Lu, Cong;Shi, Zhe;Sun, Xiuping;Pan, Ruile;Chen, Shanguang;...

期刊： Journal of Ethnopharmacology,2017年201:73-81 ISSN：0378-8741

通讯作者： Liu, XM

作者机构： [Pan, Ruile; Lu, Cong; Liu, Xinmin; Sun, Xiuping; Shi, Zhe] Chinese Acad Med Sci, Inst Med Plant Dev IMPLAD, Res Ctr Pharmacol & Toxicol, Beijing, Peoples R China.;[Pan, Ruile; Lu, Cong; Liu, Xinmin; Sun, Xiuping; Shi, Zhe] Peking Union Med Coll, Beijing, Peoples R China.;[Chen, Shanguang; Li, Yinghui; Qu, Lina] China Astronaut Res & Training Ctr, Beijing, Peoples R China.;[Dang, Haixia; Sun, Lihua] Chinese Acad Chinese Med Sci, Beijing, Peoples R China.;[Chen, Lingling; Bu, Lanlan; Liu, Xinmin] Hunan Univ Tradit Chinese Med, Changsha, Hunan, Peoples R China.

通讯机构： [Liu, XM ] C;Chinese Acad Med Sci, Inst Med Plant Dev IMPLAD, Res Ctr Pharmacol & Toxicol, Beijing, Peoples R China.;Peking Union Med Coll, Beijing, Peoples R China.;Hunan Univ Tradit Chinese Med, Changsha, Hunan, Peoples R China.

关键词： amyloid beta protein[1-40];herbaceous agent;Kai Xin San aqueous extract;unclassified drug;acetylcholine;amyloid beta protein;amyloid beta protein[1-40];glutamic acid;herbaceous agent;kai xin san;neuroprotective agent;peptide fragment;adaptive behavior;amnesia;animal experiment;animal model;Article;Chinese medicine;cognitive defect;comprehension;controlled study;herbal medicine;hippocampal CA1 region;hippocampus;information;learning;male;memory;mental performance;microinjection;nonhuman;outcome assessment;rat;reinforcement;reward;task performance;animal;animal behavior;cognition;Cognition Disorders;drug effects;hippocampus;learning;metabolism;phytotherapy;Wistar rat;Acetylcholine;Amyloid beta-Peptides;Animals;Behavior, Animal;Cognition;Cognition Disorders;Drugs, Chinese Herbal;Glutamic Acid;Hippocampus;Learning;Male;Neuroprotective Agents;Peptide Fragments;Phytotherapy;Rats, Wistar;Reward

摘要： Ethnopharmacological relevance Kai Xin San (KXS), a traditional formula of Chinese medicine, has been used to treat dementia. Aim of the study The present study aimed to investigate its ameliorating effects on Aβ1-40-induced cognitive impairment in rats using a series of novel reward-directed instrumental learning tasks, and to determine its possible mechanism of action. Materials and methods Rats were pretreated with KXS aqueous extract (0.72 and 1.44 g/kg, p.o.) for 10 days, and were trained to gain reward reinforcement by lever pressing at the meantime. Thereafter, rats received a bilateral microinjection of Aβ1-40 in CA1 regions of the hippocampus. Cognitive performance was evaluated with the goal directed (higher response ratio) and habit (visual signal discrimination and extinction) learning tasks, as well as on the levels of memory-related biochemical parameters and molecules. Results Our findings first demonstrated that KXS can improve Aβ1-40-induced amnesia in RDIL via enhancing the comprehension of action-outcome association and the utilization of cue information to guide behavior. Then, its ameliorating effects should be attributed to the modulation of memory-related molecules in the hippocampus. Conclusion In conclusion, KXS has the potential to prevent and/or delay the deterioration of cognitive impairment in AD. © 2017 Elsevier Ireland Ltd

语种： 英文

作者： Zhang, Shui-Han;Hu, Xin;Shi, Shu-Yun;Huang, Lu-Qi;Chen, Wei;...

期刊： Analytical and Bioanalytical Chemistry,2016年408(13):3659-3672 ISSN：1618-2642

通讯作者： Shi, SY;Huang, LQ

作者机构： [Chen, Wei; Chen, Lin; Cai, Ping; Zhang, Shui-Han] Hunan Acad Chinese Med, Res Inst Chinese Med, Changsha 410013, Hunan, Peoples R China.;[Shi, Shu-Yun; Shi, SY; Hu, Xin] Cent South Univ, Coll Chem & Chem Engn, Changsha 410083, Hunan, Peoples R China.;[Huang, LQ; Huang, Lu-Qi] China Acad Chinese Med Sci, Natl Resource Ctr Chinese Mat Med, Beijing 100700, Peoples R China.

通讯机构： [Shi, SY ; Huang, LQ ] C;Cent South Univ, Coll Chem & Chem Engn, Changsha 410083, Hunan, Peoples R China.;China Acad Chinese Med Sci, Natl Resource Ctr Chinese Mat Med, Beijing 100700, Peoples R China.

关键词： Chlorogenic acid;Diagnostic mass fragmentation analysis;HPLC-DAD-QTOF-MS/MS;Isomer;Lonicera macranthoides;UV spectra screening

摘要： A major challenge of profiling chlorogenic acids (CGA) in natural products is to effectively detect unknown or minor isomeric compounds. Here, we developed an effective strategy, typical ultraviolet (UV) spectra in combination with diagnostic mass fragmentation analysis based on HPLC–DAD–QTOF-MS/MS, to comprehensively profile CGA in the buds of Lonicera macranthoides. First, three CGA UV patterns were obtained by UV spectra screening. Second, 13 types of CGA classified by molecular weights were found by thorough analysis of CGA peaks using high-resolution MS. Third, selected ion monitoring (SIM) was carried out for each type of CGA to avoid overlooking of minor ones. Fourth, MS/MS spectra of each CGA were investigated. Then 70 CGA were identified by matching their UV spectra, accurate mass signals and fragmentation patterns with standards or previously reported compounds, including six caffeoylquinic acids (CQA), six diCQA, one triCQA, three caffeoylshikimic acids (CSA), six diCSA, one triCSA, three p-coumaroylquinic acids (pCoQA), four p-coumaroylcaffeoylquinic acids (pCoCQA), four feruloylquinic acids (FQA), five methyl caffeoylquinates (MCQ), three ethyl caffeoylquinates (ECQ), three dimethoxycinnamoylquinic acids (DQA), six caffeoylferuloylquinic acids (CFQA), six methyl dicaffeoylquinates (MdiCQ), four FQA glycosides (FQAG), six MCQ glycosides (MCQG), and three ethyl dicaffeoylquinates (EdiCQ). Forty-five of them were discovered from Lonicera species for the first time, and it is noted that CGA profiles were investigated for the first time in L. macranthoides. Results indicated that the developed method was a useful approach to explore unknown and minor isomeric compounds from complex natural products. [Figure not available: see fulltext.] © 2016, Springer-Verlag Berlin Heidelberg.

语种： 英文

作者： Yi Ning（宁毅）;Qiang Gao（高强）;Xiaoqing Zhang;Ke Wei（魏科）;Lingli Chen（陈伶利）

期刊： SLAS Discovery,2016年21(8):851-857 ISSN：2472-5552

通讯作者： Chen, LL

作者机构： [Ke Wei; Yi Ning; Qiang Gao; Lingli Chen; Chen, Lingli] Hunan Univ Chinese Med, Dept Microbiol, Sch Med, Xueshi Rd 300, Changsha 410208, Hunan, Peoples R China.;[Xiaoqing Zhang] Hunan Univ Chinese Med, Dept Inorgan Phys Chem, Sch Pharm, Changsha, Hunan, Peoples R China.

通讯机构： [Chen, LL ] H;Hunan Univ Chinese Med, Dept Microbiol, Sch Med, Xueshi Rd 300, Changsha 410208, Hunan, Peoples R China.

会议名称： 中华中医药学会免疫学分会第二次学术交流会暨中华中医药学会免疫学分会青年委员会成立大会

会议时间： 2016-08-27

会议地点： 深圳

会议论文集名称： 中华中医药学会免疫学分会第二次学术交流会暨中华中医药学会免疫学分会青年委员会成立大会论文集

关键词： graphene oxide;methicillin-resistant Staphylococcus aureus;mecA gene;signal amplification;biosensor

摘要： To develop new technology for detecting methicillin-resistant Staphylococcus aureus (MRSA), a novel fluorescent biosensor based on Klenow fragment (KF)-assisted target recycling amplification and synchronous fluorescence analysis was created. Carboxy-fluorescein (FAM)-labeled single-stranded DNA (ssDNA) containing a capture probe and a signal probe was adsorbed onto the surface of graphene oxide (GO) via π-stacking interactions, resulting in the fluorescence quenching of the dye. When target and primer were introduced, the fluorescence was restored due to P0 being completely released from the surface of the GO. Meanwhile, by using the KF and exploiting the synergistic effect of FAM and the double-stranded DNA (dsDNA)-SYBR Green I duplex structure, the fluorescence in this detection system was considerably amplified and the sensitivity was improved. The proposed strategy for mecA gene analysis showed a good linear range from 1 to 40 nmol/L, with a lower limit of detection of 0.5 nmol/L. In addition, a bacterial sample harboring the mecA gene was also detected, and its lower detection limit was up to 300 colony-forming units (CFU)/mL. Accordingly, this biosensor exhibits high sensitivity and selectivity and has great potential for early clinical diagnosis and treatment. © 2016 Society for Laboratory Automation and Screening.

语种： 英文

作者： Peng, Mi-Jun;Shi, Shu-Yun;Chen, Lin;Zhang, Shui-Han;Cai, Ping;...

期刊： Analytical and Bioanalytical Chemistry,2016年408(24):6693-6701 ISSN：1618-2642

通讯作者： Shi, SY;Zhang, SH

作者机构： [Peng, Mi-Jun] Jishou Univ, Key Lab Hunan Forest Prod & Chem Ind Engn, Zhangjiajie 427000, Hunan, Peoples R China.;[Shi, Shu-Yun; Shi, SY; Chen, Xiao-Qing] Cent S Univ, Coll Chem & Chem Engn, Changsha 410083, Hunan, Peoples R China.;[Chen, Lin; Cai, Ping; Zhang, SH; Zhang, Shui-Han] Hunan Acad Chinese Med, Res Inst Chinese Med, Changsha 410013, Hunan, Peoples R China.

通讯机构： [Shi, SY ] C;[Zhang, SH ] H;Cent S Univ, Coll Chem & Chem Engn, Changsha 410083, Hunan, Peoples R China.;Hunan Acad Chinese Med, Res Inst Chinese Med, Changsha 410013, Hunan, Peoples R China.

关键词： Immobilized enzyme microcolumn;High-performance liquid chromatography;Xanthine oxidase inhibitor;Caffeoylquinic acid;Natural products

摘要： Screening and analysis of bioactive compounds from natural products is challenging work due to their complexity. This study presents the first report on hyphenation of solid-phase ligand-fishing using immobilized xanthine oxidase microcolumn (IXOM) and high-performance liquid chromatography–diode array detector–tandem mass spectrometry (HPLC–DAD–MS/MS) for screening and identification of XO inhibitors from complex mixtures. Solid-phase ligand-fishing system was hyphenated withthe HPLC system via four-port switching valve and a six-port injection valve as an interface for transferring effluents from IXOM to HPLC, and collecting chromatograms from LFMC (ligand-fishing microextraction column) and C18 column in a run by only one DAD. Mixtures containing allopurinol (positive control) and tryptophane (negative control) were analyzed in order to verify the specificity and reproducibilityof the approach. Subsequently, the newly developed system was applied to screening and identification of XO inhibitors from L. macranthoides and its human microsomal metabolites. Six prototype compounds (3-caffeoylquinic acid, 5-caffeoylquinic acid, 4-caffeoylquinic acid, 3,4-dicaffeoylquinic acid, 3,5-dicaffeoylquinic acid, 4,5-dicaffeoylquinic acid) and three metabolites (3-caffeoyl-epi-quinic acid, 5-caffeoyl-epi-quinic acid, 4-caffeoyl-epi-quinic acid) with XO binding affinities were identified. The XO inhibition activities of six prototype compounds were evaluated and confirmed using in vitro enzymatic assay. With the online system developed here, we present a feasible, selective, and effective strategy for rapid screening and identification of enzyme inhibitors from complex mixtures. © 2016, Springer-Verlag Berlin Heidelberg.

语种： 英文

作者： Hu, Xin;Chen, Lin;Shi, Shuyun;Cai, Ping;Liang, Xuejuan;...

期刊： Journal of Pharmaceutical and Biomedical Analysis,2016年124(1):254-260 ISSN：0731-7085

通讯作者： Shi, SY;Zhang, SH

作者机构： [Shi, SY; Shi, Shuyun; Hu, Xin] Cent S Univ, Coll Chem & Chem Engn, Changsha 410083, Hunan, Peoples R China.;[Zhang, Shuihan; Liang, Xuejuan; Chen, Lin; Cai, Ping; Zhang, SH] Hunan Acad Chinese Med, Res Inst Chinese Med, Changsha 410013, Hunan, Peoples R China.

通讯机构： [Shi, SY ] C;[Zhang, SH ] H;Cent S Univ, Coll Chem & Chem Engn, Changsha 410083, Hunan, Peoples R China.;Hunan Acad Chinese Med, Res Inst Chinese Med, Changsha 410013, Hunan, Peoples R China.

关键词： 1,1 diphenyl 2 picrylhydrazyl;1,4 di o caffeoylquinic acid;2,2' azinobis(3 ethylbenzothiazoline 6 sulfonic acid);3,4 di o caffeoylquinic acid;3,4,5 tri o caffeoylquinic acid;3,5 di o caffeoylquinic acid;4 hydroxybenzoic acid;4 o caffeoylquinic acid;4,5 di o caffeoylquinic acid;acetic acid ethyl ester;antioxidant;butanol;butylcresol;caffeic acid;chlorogenic acid;chrysoeriol 7 o glucoside;flavonoid;gallic acid;isoquercitrin;Lonicera extract;Lonicerae macranthoides extract;luteolin;luteolin 7 glucoside;neochlorogenic acid;oxygen;petroleum ether;phenol derivative;protocatechuic acid;quercetin;unclassified drug;water;antioxidant;phenol derivative;ABTS radical scavenging assay;antioxidant activity;Article;comparative study;controlled study;diode;diode array detector;DPPH radical scavenging assay;high performance liquid chromatography;IC50;in vitro study;Lonicera;Lonicerae macranthoides;molecular weight;nonhuman;oxidation reduction reaction;priority journal;tandem mass spectrometry;chemistry;high performance liquid chromatography;Lonicera;procedures;tandem mass spectrometry;Antioxidants;Chromatography, High Pressure Liquid;Lonicera;Phenols;Tandem Mass Spectrometry

摘要： Lonicerae macranthoides with strong antioxidant activity is commonly used in traditional Chinese medicine and folk tea/beverage. However, detailed information about its antioxidant activity and bioactive compounds is limited. Then at first, we comparatively evaluated total phenolic content (TPC), total flavonoid content (TFC) and antioxidant activities of water extract, petroleum ether, ethyl acetate and n-butanol fractions of L. macranthoides. Ethyl acetate fraction exhibited the highest level of TPC (207.38 mg GAE/g DW), TFC (53.06 mg RE/g DW) and the best DPPH scavenge activity and reducing power. n-Butanol fraction showed the best ABTS+ and O2- scavenging activities. Interestingly, water extract, ethyl acetate and n-butanol fractions showed stronger antioxidant activities than positive control, butylated hydroxytoluene (BHT). After that, thirty-one antioxidant phenolic compounds, including twenty-two phenolic acids and nine flavonoids, were screened by DPPH-HPLC experiment and then identified using HPLC-DAD-QTOF-MS/MS. It is noted that twenty-one compounds (1, 3-4, 6-17, 19, 23, 26, 28-29, and 31), as far as was known, were discovered from L. macranthoide for the first time, and eleven of them (3-4, 10-17, and 23) were reported in Lonicera species for the first time. Results indicated that L. macranthoides could serve as promising source of rich antioxidants in foods, beverages and medicines for health promotion. © 2016.

语种： 英文

作者： Liu, Yan;Yang, Shenghui（杨胜辉）;Xiao, Jianhua;Yu, Liang;Chen, Li（陈丽）;...

期刊： International Journal of Clinical and Experimental Pathology,2015年8(2):1247-1258 ISSN：1936-2625

通讯作者： Zeng, QR

作者机构： [Liu, Yan; Xiao, Jianhua; Yu, Liang; Wang, Kegeng; Zou, Ju] Univ South China, Affiliated Hosp 1, Dept Parasitol, Hengyang, Peoples R China.;[Tan, Sijie] Univ South China, Affiliated Hosp 1, Dept Histol & Embryol, Sch Med, Hengyang, Peoples R China.;[Yu, Zhengyang] Univ South China, Affiliated Hosp 1, Dept Surg Oncol, Hengyang, Peoples R China.;[Yang, Shenghui] Hunan Univ Chinese Med, Sch Med, Dept Prevent Med & Pathogen Biol, Changsha, Hunan, Peoples R China.;[Chen, Li] Univ Penn, Dept Med, Perelman Sch Med, Philadelphia, PA 19104 USA.

通讯机构： [Zeng, QR ] C;Cent S Univ, Xiangya Sch Med, Ctr Cell & Mol Biol Expt, 172 Tongzipo Rd, Changsha 410013, Hunan, Peoples R China.

关键词： alkaline phosphatase;animal;Enterobacteria phage M13;liver;lung;metabolism;microbiology;mouse;Schistosoma japonicum;Alkaline Phosphatase;Animals;Bacteriophage M13;Liver;Lung;Mice;Schistosoma japonicum

摘要： The present study was to determine the targeting effect of M13 phage peptide ZL4 (MppZL4) on Schistosoma japonicum (S.j). Mice infected with S.j were injected with MppZL4. Real-time PCR was used to detect the distribution and metabolism of MppZL4 in the livers and lungs of mice. In vivo refusion test was performed to detect the targeting of MppZL4. Western blotting was employed to determine the expression of MppZL4. Live imaging was used to detect the distribution of oligopeptide MppZL4. Immunohistochemistry was employed to determine MppZL4 location on adult S.j body surface. Gomori method was employed to detect the influence of oligopeptide MppZL4 on alkaline phosphatase activity. The distribution and metabolism of MppZL4 and M13KE are not significantly different from each other at each time point. The abundance of MppZL4 is changed as S.j migrates in mice. The targeted binding effect of MppZL4 varies at different stages. ZL4 oligopeptide targets S.j in mice. The specific binding sites of MppZL4 on S.j body are mainly located in syncytial cells. The binding sites of MppZL4 on S.j body surface might be ALP or ALP-related proteins. MppZL4 had targeted binding effect on S.j with its binding site being associated with proteins related to S.j alkaline phosphatase. S.j tegument had a specifically binding site with exogenous peptides, offering new means to explore the interactions between hosts and parasites. Additionally, MppZL4 can possibly be used as targeting molecules in worm-resistant drugs or as tracing molecules in imaging diagnosis technologies.

语种： 英文

作者： Ning, Yi（宁毅）;Cheng, Lijuan（程莉娟）;Ling, Min;Feng, Xinru;Chen, Lingli（陈伶利）;...

期刊： Pathogens and Disease,2015年73(6):ftv034 ISSN：0928-8244

通讯作者： Deng, L

作者机构： [Cheng, Lijuan; Ning, Yi; Ling, Min; Wu, Minxi; Feng, Xinru; Deng, Le] Hunan Normal Univ, Coll Life Sci, Dept Microbiol, Changsha 410081, Hunan, Peoples R China.;[Cheng, Lijuan; Ning, Yi; Chen, Lingli] Hunan Univ Chinese Med, Sch Med, Changsha 410208, Hunan, Peoples R China.;[Deng, Le] Hunan Normal Univ, Coll Life Sci, Dept Microbiol, Lushan Rd 36, Changsha 410081, Hunan, Peoples R China.

通讯机构： [Deng, L ] H;Hunan Normal Univ, Coll Life Sci, Dept Microbiol, Lushan Rd 36, Changsha 410081, Hunan, Peoples R China.

关键词： aptamer;flagellin;ligand;single stranded DNA;antiinfective agent;aptamer;protein binding;Article;atomic force microscopy;bacterial cell;binding affinity;biofilm;controlled study;dissociation constant;Escherichia coli;in vitro study;mass spectrometry;maximum binding capacity;nonhuman;priority journal;Salmonella enterica;Salmonella enterica serovar Paratyphi A;Staphylococcus aureus;systematic evolution of ligands by exponential enrichment aptamer technique;biofilm;drug effects;isolation and purification;metabolism;microscopy;physiology;Salmonella;Anti-Bacterial Agents;Aptamers, Nucleotide;Biofilms;Flagellin;Microscopy;Protein Binding;Salmonella;SELEX Aptamer Technique

摘要： Biofilms are microbial communities that are attached to a solid surface using extracellular polymeric substances. Motility and initial attachment mediated by flagella are required for biofilm formation. Therefore, blocking the motility of flagella is a potential strategy to inhibit biofilm formation. In this study, single-stranded DNA aptamers specific to the Salmonella choleraesuis were selected after 14 cycles of the systematic evolution of ligands by exponential enrichment. Among the selected aptamers, the aptamer 3 showed the highest affinity for S. choleraesuis with a dissociation constant (Kd) of 41 ± 2nM. Aptamer 3, conjugated with magnetic beads, was then used to capture its binding target on the bacteria. After mass spectrometry and specific binding analysis, the flagellin was identified as the target captured by aptamer 3. Furthermore, inhibition experiments, inverted microscopy and atomic force microscopy demonstrated that aptamer 3 was able to control the biofilm formation and promote the inhibitory effect of an antibiotic on bacterial biofilms. Single-stranded DNA aptamers therefore have great potential as inhibitors of biofilm formation. © FEMS 2015. All rights reserved.

语种： 英文

作者： Hou, Qian;He, Wen-Jun;Chen, Li;Hao, Hao-Jie;Liu, Jie-Jie;...

期刊： INTERNATIONAL JOURNAL OF LOWER EXTREMITY WOUNDS,2015年14(4):335-342 ISSN：1534-7346

通讯作者： Fu, XB

作者机构： [Dong, Liang; Liu, Jie-Jie; Han, Wei-Dong; Hao, Hao-Jie; Tong, Chuan; Li, Mei-Rong; Fu, Xiao-Bing; He, Wen-Jun; Chen, Li; Hou, Qian] Chinese Peoples Liberat Army Gen Hosp, Beijing 100048, Peoples R China.;[Hou, Qian] Chinese PLA 148th Hosp, Zibo, Peoples R China.;[Fu, Xiao-Bing; He, Wen-Jun] Chinese Peoples Liberat Army Gen Hosp, Affiliated Hosp 1, Beijing 100048, Peoples R China.;[Chen, Li] Hunan Univ Chinese Med, Changsha, Hunan, Peoples R China.;[Zhou, Zhong-Zhi] Hunan Univ Chinese Med, Affiliated Hosp 1, Changsha, Hunan, Peoples R China.

通讯机构： [Fu, XB ] C;Chinese Peoples Liberat Army Gen Hosp, Affiliated Hosp 1, Wound Healing & Cell Biol Lab, 51 Fucheng Rd, Beijing 100048, Peoples R China.

关键词： beta actin;collagen type 1;collagen type 3;herbaceous agent;interleukin 6;tumor necrosis factor alpha;von Willebrand factor;wound healing promoting agent;herbaceous agent;Agrimonia pilosa;angiogenesis;animal experiment;animal model;animal tissue;Article;Boswellia sacra;controlled study;granulation tissue;immunofluorescence;in vitro study;inflammatory cell;male;medicinal plant;mouse;Nelumbo nucifera;nonhuman;Pollen typhae;priority journal;punch biopsy;real time polymerase chain reaction;streptozotocin-induced diabetes mellitus;tongue;wound healing;wound healing impairment;animal;C57BL mouse;drug effects;experimental diabetes mellitus;phytotherapy;wound healing;Animals;Diabetes Mellitus, Experimental;Drugs, Chinese Herbal;Male;Mice;Mice, Inbred C57BL;Phytotherapy;Wound Healing

摘要： Wound healing is a troublesome problem in diabetic patients. Besides, there is also an increased risk of postsurgical wound complications for diabetic patient. It has been revealed that traditional Chinese medicine may promote healing and inhibit scar formation, while the changes of morphology and physiology of wounds on such medicine treatment still remain elusive. In this study, we first used the ultralow temperature preparation method to produce mixed superfine powder from Agrimonia pilosa (A), Nelumbo nucifera (N), Boswellia carteri (B), and Pollen typhae (P), named as ANBP. Applying ANBP on 40 streptozotocin (STZ)-induced diabetic C57BL/6 mice (4-6 weeks, 20 ± 2 g), we observed that the wound healing process was accelerated and the wound healing time was shortened (14 days, P <.05). Pathological observation using hematoxylin-eosin staining indicated that inflammatory cells were reduced (P <.05) while the thickness of granulation tissue and length of epithelial tongue were increased (P <.05). The vascular density was increased on 7 and 14 days after ANBP treatment. Masson and Sirius red staining showed that, at the early stage of trauma, the expressions of Col I and Col III, especially Col III, were increased in the ANBP group (P <.05). Studies in vitro demonstrated that tubular formation was significantly increased after ANBP treatment on human vascular endothelial cells in a dose-dependent way. Taken together, our studies revealed that ANBP treatment could accelerate wound healing, promote vascularization, and inhibit inflammation, suggesting the potential clinic application of ANBP for diabetes mellitus and refractory wounds. © The Author(s) 2015.

语种： 英文

作者： Wang, Wei;Chen, Lili;Tang, Qun;Fan, Yonggang;Zhang, Xiaoru;...

期刊： Wspolczesna Onkologia,2014年18(5):329-333 ISSN：1428-2526

通讯作者： Zhai, J.

作者机构： [Zhai, Jingming; Wang, Wei; Zhang, Xiaoru; Fan, Yonggang] Departments of General Surgery, the First Affiliated Hospital, Henan University of Science and Technology, Luoyang, Henan 471000, China;[Wang, Wei; Chen, Lili] These authors contributed equally to this work;[Chen, Lili] Department of Pathology, the First Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong 510080, China;[Tang, Qun] Department of Pathology, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China

通讯机构： Departments of General Surgery, First Affiliated Hospital, Henan University of Science and Technology, 24 Jinghua Road, Luoyang, Henan, China

关键词： ARHI;colon cancer;methylation;tumour suppressor gene

摘要： AIM OF THE STUDY: The Ras-related tumour suppressor gene aplasia Ras homolog member I (ARHI) is downregulated in many types of cancer, including ovarian cancer and hepatocellular carcinoma. In the present study, we explore the expression level and role of ARHI in colon cancer. Moreover, the mechanisms that down-regulate expression of ARHI in colon cancer will be further investigated. MATERIAL AND METHODS: ARHI expression levels were evaluated with immunohistochemistry, reverse transcriptase-PCR, and western blot. Loss of heterozygosity (LOH), single strand conformation polymorphism (SSCP), and methylation-specific PCR (MSP) were used to study the mechanisms of ARHI down-regulation. RESULTS: Low expression of ARHI was observed in 61.7% (37/60) of colon cancer specimens. Compared with the paired noncancerous tissues, ARHI expression was significantly decreased in colon cancer tissues. Furthermore, low ARHI expression was significantly associated with worse differentiation degree and Dukes' stage (P < 0.05). Methylation-specific PCR assay revealed that the methylation rates of ARHI were 53.3% (16/30) and 46.7% (14/30) in ARHI CpG I and CpG II, respectively. Therefore, methylation of promoter may be involving in down regulation of ARHI expression. CONCLUSIONS: These data highlight an important role for ARHI in colon cancer, which could be a therapeutic strategy against this malignancy.

语种： 英文